Repositório RCAAP

This paper describes an application aimed at improving the current state of enforcement in the areas of food safety and economic activities in Portugal. More specifically, the application focuses on a flexible and interactive approach to generate inspection routes, to be followed by surveillance brigades with the aim of verifying Economic Operators’ compliance to national and European legislation on economic and food safety. The problem is modeled as a Multi-Depot Periodic Vehicle Routing Problem with Time Windows, and the algorithmic approaches employed seek to maximize either the number of inspected Economic Operators or a utility function that takes into account the utility gained from inspecting each Economic Operator. The generated solutions are shown in an intuitive platform, where human operators can visualize the solutions details (including georeferenced locations in a map) and fully customize them on time by manually removing or adding Economic Operators to be targeted.

In a deeply interconnected world of people and goods, infectious diseases constitute a serious threat. An active vigilance is required. The collection of adequate data is vital and coordinated by surveillance systems. It is widely-acknowledged that every case-reporting system has some degree of under-reporting and reporting delay in particular in HIVAIDS Portuguese Surveillance System. To better understand the processes generating the reporting delays, which is an administrative process, it was used a flexible continuous time fully parametric survival analysis approach. It was taken into consideration the hierarchical administrative and organizational structure of the system as well as the relevant changes in the procedures throughout the time. The best multilevel structure to represent reporting delays in continuous time is the model where the individuals are nested into Reporting Entities (20.24% of the variance) which are nested into Type of services (8% of the variance) with the log-normal distribution.

Artificial intelligence techniques have been applied to diverse business and governmental areas, in order to take advantage of the huge amount of information that is generated within specific organizations or institutions. Business intelligence can be seen as the process of converting such information into actionable knowledge, which is the basis for data-driven decision making. With this in mind, this work is framed in a project that seeks to improve the activity of the Portuguese Food and Economic Safety Authority, regarding prevention in the areas of food safety and economic enforcement. More specifically, this paper focuses on the generation and optimization of flexible inspection routes. An optimal inspection route seeks to maximize the number of targeted Economic Operators, or the utility gained from the set of Economic Operators that are actually inspected. For that, each Economic Operator is assigned an inspection utility value. The problem was then modelled as a Multi-Depot Periodic Vehicle Routing Problem with Time Windows, and solved using both exact and meta-heuristic methods. The comparison of the meta-heuristic algorithms showed a versatile Hill Climbing implementation in different test cases that explored the effect of the Economic Operators dispersion and density.

Data-driven decision support systems rely on increasing amounts of information that needs to be converted into actionable knowledge in business intelligence processes. The latter have been applied to diverse business areas, including governmental organizations, where they can be used effectively. The Portuguese Food and Economic Safety Authority (ASAE) is one example of such organizations. Over its years of operation, a rich dataset has been collected which can be used to improve their activity regarding prevention in the areas of food safety and economic enforcement. ASAE needs to inspect Economic Operators all over the country, and the efficient and effective generation of optimized and flexible inspection routes is a major concern. The focus of this paper is, thus, the generation of optimized inspection routes, which can then be flexibly adapted towards their operational accomplishment. Each Economic Operator is assigned an inspection utility – an indication of the risk it poses to public health and food safety, to business practices and intellectual property as well as to security and environment. Optimal inspection routes are then generated typically by seeking to maximize the utility gained from inspecting the chosen Economic Operators. The need of incorporating constraints such as Economic Operators’ opening hours and multiple departure/arrival spots has led to model the problem as a Multi-Depot Periodic Vehicle Routing Problem with Time Windows. Exact and meta-heuristic methods were implemented to solve the problem and the Genetic Algorithm showed a high performance with realistic solutions to be used by ASAE inspectors. The hybrid approach that combined the Genetic Algorithm with the Hill Climbing also showed to be a good manner of enhancing the solution quality.

Tradução de “Das dicke Kind de Marie Luise Kaschnitz”

Methicillin-resistant Staphylococcus aureus (MRSA) is a highly pathogenic multiple-drug resistant (MDR) microorganism that is most prevalent in the community. It has been found that MRSA strains can also contain genes that encode the panton valentine leukocidin toxin (PVL) and several genes that confer resistance to a various number of antimicrobial agents. The aim of this study was to determine the presence of MRSA (mecA gene) and PVL toxin genes (lukS-PV and lukF-PV) in strains isolated from two hospitals. The susceptibility to a various number of antimicrobial agents was tested and its clonality also studied. The results indicated a frequency of approximately 50% of MRSA and a frequency of 9% of isolates carrying the lukFS-PVL gene. Regarding the susceptibility patterns of isolates under study it was concluded that for most antimicrobial agents used MRSA strains reveal to be resistant to more antimicrobials than the isolates that lack mecA gene. Regarding fingerprinting method it was possible to observe a quite fixed number of strains among pathogen causing hospitalacquiring infection. The explanation for these findings is complex and multi-factorial. The high diversity of clones suggests diverse genetic backgrounds rather than the global spread of a single clone.

5-Methoxy-N,N-dimethyltryptamine(5-MeO-DMT), lysergic acid diethylamide (LSD), and mescaline are classic hallucinogens known for their recreational use, which increased in the last dec-ades. Despite some available data on the metabolism of these drugs [1-3], a scientific gap exists regarding their possible interactions with CYP450 enzymes. Nevertheless, this information is of crucial relevance to predict drug-drug interactions and understand toxicological phenomena, in particular interindividual variability. This study aimed to evaluate in vitroand in silicothe interaction of 5-MeO-DMT, LSD, and mescaline with the enzymes CYP2A6/2B6/2D6/2E1/3A4. The in vitroassessment of CYP450 inhibition was performed using the Vivid®CYP450 screening kits. IC50 was calculated using GraphPad Prism 9.3.0. For in silicoassessment, molecular dynamics were performed using the PMEMD.cuda module in AMBER16. Calculations were made on the last 100 ns of the trajectory (stable zone) to assess the interaction mode/strength between enzyme and ligand, namely MMGBSA, per-residue decomposition energy, and hydrogen bonds. Based on the IC50(μM), LSD (0.35) and 5-MeO-DMT (3.47) present the capacity to be inhibitors of CYP2D6. Based on the MMGBSA (kcal/mol), LSD showed the highest binding affinities for all enzymes, while mescaline showed the lowest. The strong interaction of LSD with CYP2A6 is mediated by a hydrogen bond established with the protein residue Asn297. For interaction with CYP2B6, the residues Thr302 and Lys479 were important in mediating the interaction with 5-MeO-DMT and LSD. Key residues mediating the interaction of 5-MeO-DMT and LSD with CYP2D6 included Phe120, Leu213, and Phe483. For interaction with CYP2E1, residues Phe207, Phe298, and Thr303 are important; and for CYP3A4, an important hydrogen bond between LSD and Ala370 was identified.Conclusions: Both LSD and 5-MeO-DMT are predicted to have strong potential to be CYP2D6 inhibitors. A strong interaction was also identified in silicobetween LSD and CYP2A6.

Psilocybin is a hallucinogen produced by “magic mushrooms”, being rapidly metabolized in the organism into psilocin [1, 2]. A scientific gap exists regarding the possible interactions between psilocybin/psilocin and CYP450 enzymes. Given their biological importance, and since the binding of drugs to CYP450 enzymes can interfere with the metabolism of other substrates leading to drug-drug interactions, this research topic is of utmost importance. This study aimed to evaluate in silicoand in vitrothe interaction of psilocybin and psilocin with the enzymes CYP2A6/2B6/2D6/2E1/3A4. The in vitroassessment of inhibition was performed using the Vivid®CYP450 screening kits. IC50 was calculated using GraphPad Prism 9.3.0. For in silico assessment, molecular dynamics were per-formed using the PMEMD.cuda module in AMBER16. Calculations were made on the last 100 ns of the trajectory (stable zone) to assess the interaction between enzyme and ligand, namely MMGBSA, per-residue decomposition energy, and hydrogen bonds. Psilocin showed the capacity to be an in-hibitor of CYP2A6/2B6/2D6/2E1/3A4, based on the respective IC50 values (μM) of 2.06, 6.17, 11.89, 6.37, and 2.36. Considering the MMGBSA, higher values were obtained for psilocin, corroborating the stronger binding affinity of this compound. The interaction of psilocybin/psilocin with CYP2A6 is medi-ated by a hydrogen bond established with the protein residue Asn297. Other important residues include Phe107 and Ile366. For CYP2B6, the strong binding of psilocin is mediated by interactions with Ile114, Thr302 (hydrogen bond), and Leu363. For interaction with CYP2D6, the most important residue seems to be Ser304, with which it forms a hydrogen bond; for CYP2E1, key residues include Phe207, Thr303, and Phe478. A strong hydrogen bond is formed between psilocin and CYP3A4 residue Phe304, contrib-uting to the high binding affinity. The results suggest a potential for psilocin to inhibit all enzymes, especially CYP2A6 and CYP3A4.

For the last few years, Ayahuasca ceremonies have been gaining popularity in recreational settings in Europe and North America [1]. Similar to Psychotria viridis, Diplopterys cabreranais also suggested to contain the psychoactive compound N,N-dimethyltryptamine, and is therefore used in Aya-huasca rituals for its ability to induce hallucinations, euphoria and entheogenic effects [1-3]. However, while information on the toxic profile of D. cabreranaremains very limited, its acquisition is easily ac-complished by consumers. We aimed to characterize the aqueous extracts of D. cabreranaleaves, mimicking those typically consumed, to identify bioactives that underlie the psychoactive or toxic effects, and evaluate their impact on neuronal function, neurotransmission and radical stress. Chemical characterization was attained by HPLC-DAD. Impact upon neuronal viability was assessed by the MTT assay (up to 1000 μg/mL) in SH-SY5Y neuroblastoma cells. Impact on neuromodulation and neuroinflammation was evaluated through acetylcholinesterase and 5-lipoxygenase inhibition, while an-tiradical properties were assessed by evaluating nitric oxide (•NO) and xanthine oxidase (XO) activity. Inhibition of the α-glucosidase enzyme was also evaluated. Statistical comparisons among groups per-formed by one-way ANOVA followed by Dunnett post hoc test. Preliminary characterization results revealed the presence of several catechin derivates, alongside two apigenin derivates and one tryp-tamine derivate. Cytotoxicity was not verified up to the highest concentration tested. Acetylcholinesterase inhibition was recorded starting at 250 μg/mL, and a concentration-dependent inhibition of 5-lipoxygen-ase was found (IC50=79.77 μg/mL). Concentration-dependent scavenging effects upon •NO and XO inhi-bition were verified at concentrations higher than 1.953 μg/mL and 31.25 μg/mL, respectively. At last, inhibition of α-glucosidase occurred with concentration-dependency and an IC50of 4.78 μg/mL. Conclu-sions:Although antiradical, anti-inflammatory and antidiabetic properties were verified, with no in vitrocytotoxicity being detected, further research is needed to elucidate the underlying mechanisms that might be involved in our preliminary results.

1,3-Dimethylamylamine (1,3-DMAA),also known as methylhexanamine, is a central nervous system stimulant with structural similarities with amphetamines and therefore presenting over-lapping biological and detrimental effects [1]. Despite being banned, the presence of 1,3-DMAA in dop-ing controls and dietary supplements continues to be of significant concern. This molecule has two stere-ogenic centres and thus four stereoisomers [2]. It is widely recognized that enantiomers may exhibit dif-ferent biological activity, including pharmacokinetics, pharmacodynamics, and toxicity. Consequently, the development of analytical methods for enantioselective separation of 1,3-DMAA is crucial for an accurate determination of the risks associated with each of these stereoisomers. To develop an indirect method by gas chromatography coupled to mass spectrometry (GC-MS) for the separation and identification of the stereoisomers of the 1,3-DMAA. 1,3-DMAA was regenerated with sodium hydroxide, extracted with 0.1% triethylamine in hexane and then derivatized using the enantiomeric pure reagent (R)-(-)-α-methoxy-α-(trifluoromethyl)phenylacetyl chloride ((R)-MTPA-Cl). Subsequently, the sample was evaporated, reconstituted in anhydrous ethyl acetate, and analyzed by GC-MS. The chroma-tographic conditions were established using a capillary column containing 5% diphenyl-95% dime-thylpolysiloxane (30 m × 0.25 mm × 0.25 μm), an injector temperature set to 280 ºC, with a temperature ramp starting at 140 ºC and increasing up to 215 ºC at a flow rate of 1 mL/min to a total run of 12.32 min. Results: As preliminary data indicate, the derivatization procedure allowed the formation of 4 diastere-omers of 1,3-DMAA. The chromatographic conditions were optimised, allowing for the separation of the four diastereomers within 12 min. Derivatization and chromatographic conditions were established for enantioselective separation of 1,3-DMAA by GC-MS. Further validation of the method will be crucial for understanding the diastereomers' differential pharmacokinetics and pharmacodynamics, and consequently, the perils associated with their presence in food supplement samples.

Among the array of new psychoactive substances, synthetic cannabinoids (SC) stand out as highly popular among consumers. These substances closely resemble, in terms of their pharmacology, Δ9-tetrahydrocannabinol (THC), cannabis’ main active principle, albeit exhibiting full agonism at the cannabinoid receptors 1 and 2. In light of recent scientific findings suggesting that cannabis use can exacerbate ageing-related parameters, the present work was designed to explore whether SC share similar potential effects. For this purpose, we employed two distinct in vitro models. The first model involved primary hippocampal cultures (PHC), isolated from Wistar rat embryos at embryonic day 18–19; after seeding, cells were kept in culture and exposed to the popular SC AMB-FUBINACA (AMB-FUB) at 1 pM, 1 nM and 1 µM, starting at day-in-vitro (DIV) 3 or DIV7, and perpetuated until DIV21. DMSO at 0.02% was used as the solvent control. At the end of the exposure, ß-galactosidase activity (a common first-line cell senescence biomarker) was assessed using a commercially-available kit. Our findings under these experimental conditions, revealed that PHC exposed to all AMB-FUB concentrations had less ß-galactosidase activity than the control condition (p<0.01, 1 pM; p<0.001, 1 nM and 1 µM). The other in vitro model used herein was the human neuroblastoma cell line SH-SY5Y. Beginning at passage 24, cells were seeded and exposed to 1 nM and 1 µM AMB-FUB. At passages 24 (48h after drug exposure) and 28, samples were collected for the analysis of several senescence-related endpoints, namely ß-galactosidase activity, cell cycle analysis (via flow cytometry, following DNA staining with propidium iodide) and relative telomere length measurement (using qPCR). Surprisingly, no discernible effect of AMB-FUBINACA was observed for any of the endpoints examined. The apparent observed “anti-ageing” effect of AMB-FUB on PHC warrants further investigation. Moreover, the differential reponse observed between the two in vitro models also requires scrutiny, particularly in light of recent published findings suggesting that THC has the potential to increase ß-galactosidase activity. Further experiments will ensue to hopefully shed some light on these interesting results.

To date, no neuroprotective/disease-modifying strategy has been approved as a Parkinson’s Disease (PD) therapy, because of the‘one-disease-one-target’ view that has been followed. New drug-based therapeutic routes, namely Safinamide, have been introduced as a promising multimodal drug combining dopaminergic and non-dopaminergic (neuroprotective) actions, representing a new potential alternative therapy to prevent or delay PD progression. Thus, the present work addressed Safinamide's impact on PD, relying on the possibility of potentiating dopaminergic neurons (DAn) survival by tackling cellular/molecular impairments responsible for its failure. Safinamide (10mg/kg) was given by oral gavage to a 6-OHDA pre-clinical rat model. DAn survival, neuroinflammation, and redox system homeostasis were assessed by histological and molecular analysis. Additionally, to overpass the selective blood-brain barrier (BBB) permeability, which reduces drug bioavailability reaching PD brain regions, we conducted magnetic resonance imaging (MRI)-guided focused ultrasound (FUS) to transiently open the BBB to precisely deliver Safinamide in PD-affected areas. Results revealed that Safinamide monotherapy was able to potentiate the densities of DAn and fibers, revealing a protective effect when compared to the untreated group. To understand possible pathways associated with this improvement, we found that Safinamide appears to be a modulator of the antioxidant and autophagy systems since an increase in the expression levels of DJ-1, SOD-1, and LC3B was observed when compared to the non-treated group. Furthermore, Safinamide presents a potential modulatory activity on neuroinflammation and astrogliosis, as a decrease in microglia (CD11b+) and astrocytic (GFAP+) cells number was observed when compared to 6-OHDA group. Additionally, the anatomical and functional MRI analysis exhibited connectivity and metabolite alterations. Collectively, these data demonstrate the promising therapeutic potential of Safinamide as a neuroprotection strategy for PD, which may open new therapeutic opportunities for individuals in prodromal stages, potentially delaying clinical manifestation in high-risk patients.

Electronic cigarettes (E-cigs) and heated tobacco products (HTPs) have gained popularity as alternatives to traditional tobacco products (TTPs), claiming to reduce harm. The carcinogenic proper-ties of chemicals in the smoke of TTPs are widely recognized. However, there is still an incomplete understanding of the different chemicals in E-cigs and HTPs and their toxicity to human cells [1]. Thus, this study aimed at characterizing and comparing the chemical composition of three different brands of E-cigs, HTPs and TTPs. We selected the three top-selling brands of E-cigs, HTPs, and TTPs in Portugal, and each brand (n=9) was analyzed in triplicate. Volatile compounds present in all brands were extracted by headspace solid-phase microextraction (HS-SPME) and solvent extraction (di-chloromethane). The volatile compounds in the headspace and solvent extracts were analysed by gas chromatography-mass spectrometry (GC-MS). Compound annotation was performed by comparing the mass spectrum of each chromatographic peak in the sample with a mass spectral library and standards, where available. A total of 53 compounds were detected in E-cigs, 44 in HTPs and 41 in TTPs by HS-SPME. Solvent extraction revealed 43 compounds in E-cigs, 35 in HTPs and 22 in TTPs. Only 7 compounds were common to E-cigs, HTPs, and TTPs. Overall, the chemical classes included alcohols, aldehydes, ketones, esters, pyridines and others.The composition of HTPs and TTPs was similar (20 compounds in common), particularly in the classes of ketones, alcohols, terpenoids, and pyridines. In contrast, E-cigs contain a larger number of compounds than HTPs and TTPs, including several alcohols, esters, pyranones, and lactones. The volatile composition of HTPs and TPPs showed less variation be-tween different brands, whereas E-cig brands showed greater variability in their composition. HTPs have a volatile chemical composition similar tothat of TTPs in their original form, so their health effects will depend on the impact of the different types of combustion. E-cigs show a distinct chemical profile across all brands, with chemical classes that are potentially relevant for toxicological studies.

ADB-FUBINACA (ADB-FUB) is a synthetic cannabinoid (SC) that has gained popularity among users as a new psychoactive substance. This stems from SC's pharmacological similarity to the active principle of cannabis, D9-tetrahydrocannabinol (THC). However, unlike THC, SCs demonstrate full agonism of cannabinoid receptors 1 and 2 [1]. Recent scientific developments have shown that can-nabis use may aggravate ageing-related parameters [2,3]. Moreover, a study using human fibroblasts re-vealed that 1 μM THC (2h-longexposure, for 15 days) can increase ß-galactosidase activity [3], which serves as a first-line marker for cellular senescence. This study was designed to investigate whether these biologically-relevant concentrations could accelerate neuronal ageing. PHC were isolated from Wistar rat day 18-19 embryos and cultured for up to 21 days-in-vitro (DIV). Exposure to 1 pM, 1 nM and 1 μM ADB-FUB (concentrations previously shown to be non-cytotoxic to PHC) started either on DIV3 or DIV7 and was maintained up to 21 DIV. At that final timepoint, ß-galactosidase activity was evaluated. DMSO at 0.02% was employed as solvent control. Under these experi-mental conditions, PHC exposed to 1 nM and 1 μM ADB-FUB in the DIV3-21 protocol had lower ß-galactosidase activity when compared to control conditions (p<0.05, 1 nM; p<0.001, 1 μM). No statisti-cally significant results were registered for PHC under the DIV7-21 exposure protocol. These findings are, to the best of our knowledge, the first evidence of a potential “anti-ageing” effect of ADB-FUB. Evaluation of other senescence-related endpoints will follow. Moreover, experiments using another in vitroneuronal model (human neuroblastoma cell line SH-SY5Y) are underway to compare the effects of the same drug in different models and further substantiate conclusions on ADB-FUB’s effect.

There has been a surge in global attention to New Psychoactive Substances (NPS) [1]. Synthetic cathinones stand out as a widely consumed NPS class. Notably, 3-chloromethcathinone (3-CMC) accounted for over 34% of NPS seizures in 2021 [2], which underscores concerns regarding its consumption and health effects. Of note, 3-CMC is chiral and mostly sold as a racemate. As human me-tabolism and pharmacological effects can be enantioselective [3], determination of the impact of enanti-oselectivity in toxicokinetics/toxicodynamics is essential for the assessment of 3-CMC effects. This work aimed to evaluate in vivothe enantioselective biodistribution and toxicity of racemic 3-CMC, after an acute exposure to 3-CMC. Ten-week-old male Wistar rats were administered intraperitoneally with saline or 3-CMC (10 or 20 mg/kg; n=6). Twenty-four hours after, animals were deeply anesthetized and nine organs (brain, liver, kidneys, lungs, heart, spleen, gut, muscle, adipose tis-sue), blood and urine were collected. For evaluation of the enantiomeric biodistribution, a previous in houseestablished indirect method by gas chromatography [3], was adapted and validated. Some biochem-ical analysis was performed using an analyser, whereas TBARS, ATP, glutathione and total protein were determined by spectrophotometry. Organs were also processed for histological analysis. After 24 h, 3-CMC was not found in most organs. Both enantiomers were detected in urine with one dominant enantiomer, suggesting enantioselectivity in metabolism. The histopathological results showed possible central chromatolysis in the brain (20 mg/kg), liver inflammation, renal lesions, lungs’ haemoptysis, and alveolar haemorrhage, in most 3-CMC-exposed animals. No differences were observed inthe heart. Our findings show rapid 3-CMC renal elimination, with enantio selectivity in metabolism. Alt-hough biochemical evaluations are ongoing, the results are expected to give further insights on the 3-CMC toxicity and histological abnormalities found in the brain, kidneys, liver and lungs.

Desde a II Guerra Mundial que a língua inglesa é a língua mais traduzida em todo o mundo, mas a língua para a qual menos se traduz. Uma situação que identifica a tradução como um potencial local de variação. ‘Since World War II , English is the most translated language worldwide and one of the least translated into (Venuti 1995 a: 12-14 ), a situation that identifies translation as a potential site of variation.’ (The Scandals of Translation – Towards an ethics of difference, Lawrence Venuti, New York, 1998) Com este trabalho de projeto quis mostrar que a tradução de literatura portuguesa para a língua inglesa pode tornar-se tão interessante e motivadora como o contrário. Com este objetivo em mente, o meu trabalho baseou-se na tradução para a língua inglesa dos cinco capítulos finais de uma obra literária da minha autoria: ‘Nunca te vi, mas posso sentirte…’ As metodologias encontradas para a elaboração do trabalho foram a pesquisa de autores portugueses que proferissem conceitos relacionados com o tipo de literatura da minha obra, assim como pesquisas on line do género de leitor que aprecia este tipo de literatura. Ao nível da estrutura da tradução, apliquei técnicas e conceitos aprendidos ao longo do Curso de Mestrado e pesquisei autores estrangeiros de forma a puder fundamentar as minhas escolhas de tradução. Dividi este trabalho pela Introdução, dois capítulos, a Conclusão e a Bibliografia. Na Introdução faz-se uma apresentação muito breve do trabalho. No primeiro capítulo temos a biografia da autora, o papel que a escrita tem na sua vida e é feita uma descrição pormenorizada da obra literária que foi traduzida. São caracterizadas também todas as personagens envolvidas na obra e todo o enredo que antecede a tradução. É focado também o mito da ascensão social pela qual passa a personagem principal da obra, bem como as diferenças existentes entre Norte e Sul, quer a nível do nosso país, quer a nível mundial que achei relevantes serem argumentados visto terem ligação com o enredo da obra. No segundo capítulo temos a tradução em língua inglesa, inicia no capítulo XIII e termina no capítulo XVII, o último capítulo da obra. Em todos os capítulos existem as notas de rodapé onde são explicadas as várias opções de tradução, quer de cariz linguístico, quer de cariz literário. As opções foram devidamente fundamentadas por autores/ críticos estudados durante a execução do trabalho e houve também o recurso a citações encontradas na Internet, recurso a dicionários on line e de papel. Na Conclusão faz-se o balanço final do que foi feito, bem como todo o enriquecimento adquirido pela elaboração deste trabalho, assim como o seu contributo para o futuro. A última parte deste trabalho é composta pela bibliografia. Em primeiro lugar foi colocada toda a bibliografia encontrada nos livros que foram alvo de estudo e dicionários de papel. De seguida a bibliografia encontrada na Internet, desde citações a dicionários on line. Como anexos temos o livro com a história no original.

Tradução de contos russos retirados do livro "Читаем по-русски" de Natália Vladimirovna

O Grupo de trabalho de Educação e Curricula para a Sustentabilidade definiu no seu plano de atividades para 2024 a realização de um ciclo de webinars denominado “Horizontes Futuros: repensar o ensino e curricula para a sustentabilidade no ensino superior”. Estes eventos são de periodicidade trimestral, na forma de mesa-redonda e abordam a implementação da sustentabilidade no ensino e curricula nas Instituições de Ensino Superior (IES), em particular as que subscreveram a Carta de Intenções com a Rede Campus Sustentável (RCS). Tendo por base a comunicação para a sustentabilidade, estes webinars têm como objetivo proporcionar momentos de partilha de experiências, práticas de sucesso e de desafios que as IES encontraram nesta implementação. Para tal são convidados membros da governança das IES, de norte a sul de Portugal, para reportar o que têm feito no âmbito da missão definida neste contexto. O objetivo deste trabalho é fazer um balanço destes webinars e realçar através da análise de conteúdo das suas apresentações e dos debates ocorridos, quais têm sido as práticas mais comuns, os atuais desafios e as dificuldades nas diversas IES.

O Instituto Politécnico do Porto (P.PORTO) enquanto Instituição de Ensino Superior (IES) de referência está fortemente alinhado com o desenvolvimento sustentável assumindo um compromisso com a inovação e a sustentabilidade organizacional. De acordo com os Objetivos do Desenvolvimento Sustentável (ODS) da Agenda 2030 das Nações Unidas, o P.PORTO tem adotado uma abordagem abrangente para a promoção da sustentabilidade nas suas diversas dimensões e atividades, assim como na área da Segurança e Saúde no Trabalho (SST), promovendo ambientes de trabalho saudáveis e seguros. Centrado no ODS 8 ‘Trabalho Decente e Crescimento Económico’, visando a promoção do crescimento económico sustentado, inclusivo e sustentável e o emprego pleno e produtivo, o P.PORTO através da sua Política de Gestão, tem desenvolvido várias iniciativas, destacando-se, o plano de valorização e progressão do pessoal, o projeto ‘missão equidade, diversidade e inclusão’ e o programa de vigilância da saúde do trabalhador e do estudante. Neste contexto, e no ano 2024, foram desenvolvidas várias atividades de avaliação de risco e atividades de promoção da saúde do trabalhador e do ambiente, tendo constituído o objetivo do presente estudo. A figura 1 de forma global identifica o contributo das diferentes atividades para os ODS.

Storytelling is an effective evidence-based practice as an accepted intervention by therapists for the therapy of children with autism spectrum disorder (ASD). Digital storytelling, particularly using smart tangibles, offers a structured, interactive and engaging environment for children with ASD allowing for repetition, offering feedback with visual supports, and giving the child more authority over the learning experience. This study presents a co-designed approach to digital storytelling activities with smart tangibles for autism therapy, aimed at enhancing multiple social and behavioral skills. Through co-design sessions with therapists, activity flows and scenarios were developed to target specific skill improvements. These include free play exploration, positive stimulus introduction, fostering cooperation to address disturbances, and incorporating magical objects to facilitate peer turn-taking. Additionally, real-life connections were emphasized to promote emotional regulation and multicultural understanding while further activities are designed to overcome routine issues, build tolerance to change, and enhance cognitive structuring. Feasibility was demonstrated through integration into therapy sessions of five children, where therapists independently utilized the system, fostering immersive and interactive storytelling experiences. Overall, the co-designed activities offer insights into enhancing therapy interventions for children with ASD beyond specific contexts, contributing to the broader design of autism therapy activities.