Repositório RCAAP

The Camaquã Copper Mines (CCM) were the main sulphide deposit in Southern Brazil and have been in operation from last century to 1996. To evaluate water contamination and environmental risk increase by heavy metals from mining operations, two points on the João Dias Creek were sampled (Station 1, background area and Station 2, contaminated area). Mining activity increased the natural weakly heavy metal fluxes by approximately 5424 kg. ( ~ 60%) of the total metal flux, 1542 kg. ( ~ 49%) of dissolved and 3881 kg ( ~ 66%) of particulate metal flux. Total metal flux of anthropic origin was mostly due to Fe followed by Cu > Zn > Mn whereas Cd, As and Pb fluxes were negligible. The potential human health hazards and risk assessment related to daily intake of water from João Dias Creek are mostly due to Mn and should be of concern for the contaminated area. The ingestion of water from station 2 represents incremental risks of 130% and 59% respectively, considering the non-carcinogenic and the carcinogenic effects. The real increase of human health hazards may be greater than those related to the total concentrations since Mn and As dissolved concentrations were 5.5 and 2.0 higher than acceptable, respectively.

The waterfalls of the Presidente Figueiredo municipality represent a fascinating natural scenery of northeast state of Amazonas, northern Brazil. The falls, generally less than 10m high, are developed on siliciclastic rocks of the Nhamundá (Lower Silurian), and Manacapuru (Upper Silurian - Lower Devonian) formations. Morphological and structural analyses of these features indicate that most of them originated through Quaternary neotectonics and are installed in NE-trending normal fault escarpments. Waterfalls also developed within pseudokarstic features, but are less frequent. The origin of the Presidente Figueiredo waterfalls probably goes back to the Neogene, when the region was submitted to laterization processes associated with a humid climate and a dense rainforest. These conditions favored the development of caves in quartzarenites of the Nhamundá Formation. During the Quaternary, the region was subjected to NE-trending normal faulting which displaced laterite layers, rivers and streams giving rise to waterfalls. These climatic and tectonic phenomena promoted intense relief dissection, as indicated by fault escarpment retreat and cave dismantlement, responsible for the present-day morphologic configuration.

We study the perceptual problem related to image quantization from an optimization point of view, using different metrics on the color space. A consequence of the results presented is that quantization using histogram equalization provides optimal perceptual results. This fact is well known and widely used but, to our knowledge, a proof has never appeared on the literature of image processing.

This work establishes a connection between gravitational quantum cohomology and enumerative geometry of rational curves (in a projective homogeneous variety) subject to conditions of infinitesimal nature like, for example, tangency. The key concept is that of modified psi classes, which are well suited for enumerative purposes and substitute the tautological psi classes of 2D gravity. The main results are two systems of differential equations for the generating function of certain top products of such classes. One is topological recursion while the other is Witten-Dijkgraaf-Verlinde-Verlinde. In both cases, however, the background metric is not the usual Poincaré metric but a certain deformation of it, which surprisingly encodes all the combinatorics of the peculiar way modified psi classes restrict to the boundary. This machinery is applied to various enumerative problems, among which characteristic numbers in any projective homogeneous variety, characteristic numbers for curves with cusp, prescribed triple contact, or double points.

Let M be an n-dimensional closed minimally immersed hypersurface in the unit sphere Sn + 1. Assume in addition that M has constant scalar curvature or constant Gauss-Kronecker curvature. In this note we announce that if M has (n - 1) principal curvatures with the same sign everywhere, then M is isometric to a Clifford Torus <img src="http:/img/fbpe/aabc/v73n3/03ab.gif" alt="03ab.gif (725 bytes)" align="middle">.

An erratum to Lemma 2.1 in Do Carmo and Zhou (2000) is presented.

Cell volume changes are associated with alterations of intrinsic optical signals (IOS). In submerged brain slices in vitro, afferent stimulation induces an increase in light transmission. As assessed by measurement of the largely membrane impermeant ion tetramethylammonium (TMA) in the extracellular space, these IOS correlate with the extent and time course of the change of the extracellular space size. They have a high signal to noise ratio and allow measurements of IOS changes in the order of a few percent. Under conditions of reduced net KCl uptake (low Cl solution) a directed spatial buffer mechanism (K syphoning) can be demonstrated in the neocortex with widening of the extracellular space in superficial layers associated with a reduced light transmission and an increase of extracellular K concentration. The nature of the IOS under pathophysiological conditions is less clear. Spreading depressions first cause an increase of light transmission, then a decrease. Such a decrease has also been observed following application of NMDA where it was associated with structural damage. Pharmacological analyses suggest that under physiological conditions changes of extracellular space size are mainly caused by astrocytic volume changes while with strong stimuli and under pathophysiological conditions also neuronal swelling occurs. With reflected light usually signals opposite to those observed with transmitted light are seen. Recording of IOS from interface slices gives very complex signals since under these conditions an increase of light transmission has been reported to be superimposed by a decrease of the signal due to mechanical lensing effects of the slice surface. Depending on the method of measurement and the exact conditions, several mechanisms may contribute to IOS. Under well defined conditions IOS are a useful supplementary tool to monitor changes of extracellular volume both in space and time.

The brain is an excitable media in which excitation waves propagate at several scales of time and space. ''One-dimensional'' action potentials (millisecond scale) along the axon membrane, and spreading depression waves (seconds to minutes) at the three dimensions of the gray matter neuropil (complex of interacting membranes) are examples of excitation waves. In the retina, excitation waves have a prominent intrinsic optical signal (IOS). This optical signal is created by light scatter and has different components at the red and blue end of the spectrum. We could observe the wave onset in the retina, and measure the optical changes at the critical transition from quiescence to propagating wave. The results demonstrated the presence of fluctuations preceding propagation and suggested a phase transition. We have interpreted these results based on an extrapolation from Tasaki's experiments with action potentials and volume phase transitions of polymers. Thus, the scatter of red light appeared to be a volume phase transition in the extracellular matrix that was caused by the interactions between the cellular membrane cell coat and the extracellular sugar and protein complexes. If this hypothesis were correct, then forcing extracellular current flow should create a similar signal in another tissue, provided that this tissue was also transparent to light and with a similarly narrow extracellular space. This control tissue exists and it is the crystalline lens. We performed the experiments and confirmed the optical changes. Phase transitions in the extracellular polymers could be an important part of the long-range correlations found during wave propagation in central nervous tissue.

The ability to introduce genetic modifications in the germ line of complex organisms has been a long-standing goal of those who study developmental biology. In this regard, the mouse, a favorite model for the study of the mammals, is unique: indeed not only is it possible since the late seventies, to add genes to the mouse genome like in several other complex organisms but also to perform gene replacement and modification. This has been made possible via two technological breakthroughs: 1) the isolation and culture of embryonic stem cells (ES), which have the unique ability to colonize all the tissues of an host embryo including its germ line; 2) the development of methods allowing homologous recombination between an incoming DNA and its cognate chromosomal sequence (gene ''targeting''). As a result, it has become possible to create mice bearing null mutations in any cloned gene (knock-out mice). Such a possibility has revolutionized the genetic approach of almost all aspects of the biology of the mouse. In recent years, the scope of gene targeting has been widened even more, due to the refinement of the knock-out technology: other types of genetic modifications may now be created, including subtle mutations (point mutations, micro deletions or insertions, etc.) and chromosomal rearrangements such as large deletions, duplications and translocations. Finally, methods have been devised which permit the creation of conditional mutations, allowing the study of gene function throughout the life of an animal, when gene inactivation entails embryonic lethality. In this paper, we present an overview of the methods and scenarios used for the programmed modification of mouse genome, and we underline their enormous interest for the study of mammalian biology.

Developmental biology and evolutionary biology are both mature integrative disciplines which started in the 19th century and then followed parallel and independent scientific pathways. Recently, a genetical component has stepped into both disciplines (developmental genetics and evolutionary genetics) pointing out the need for future convergent maturation. Indeed, the Evo-Devo approach is becoming popular among developmental biologists, based on the facts that distant groups share a common ancestry, that precise phylogenies can be worked out and that homologous genes often play similar roles during the development of very different organisms. In this essay, I try to show that the real future of Evo-Devo thinking is still broader. The evolutionary theory is a set of diverse concepts which can and should be used in any biological field. Evolutionary thinking trains to ask « why » questions and to provide logical and plausible answers. It can shed some light on a diversity of general problems such as how to distinguish homologies from analogies, the costs and benefits of multicellularity, the origin of novel structures (e.g. the head), or the evolution of sexual reproduction. In the next decade, we may expect a progressive convergence between developmental genetics and quantitative genetics.

Systematic geological studies performed in the study area allowed the characterization of six lithodemic units: three gneissic, one amphibolitic, one supracrustal and one fissure mafic. The mineral assemblage and the structural record of these lithodemic units indicate that the study area was affected by five tectonothermal events. The structural pattern of the first and oldest event occurred under granulite facies conditions and reveals essentially a sinistral kinematic pattern. The second event, showing dominant extensional characteristics, is related to the generation of an ensialic basin filled by the volcano-sedimentary sequence of the supracrustal lithodemic unit. The third event, which is the most expressive in the study region, is characterized by a vigorous regional migmatization process and by the generation of the Claudio Shear Zone, presenting dextral kinematic movement. The fourth event is represented by a fissure mafic magmatism (probably two different mafic dike swarms) and finally, the fifth event is a regional metamorphic re-equilibration that reached the greenschist facies, closing the main processes of the tectonic evolution of the Campo Belo Metamorphic Complex.

The Barreiras Group is a Miocene to Lower Pleistocene continental terrigenous sedimentary deposit exhibiting a large occurrence along the Brazilian coast. In the Conde region, located in the northeastern part of the State of Bahia, the sedimentological characteristics of these sediments are indicative of a deposition as gravelly and sandy bed load in braided fluvial systems, related to alluvial fans, under an arid to semi-arid climate. The basal portion of the group is dominated by a gravelly-sandy lithofacies deposited by debris flows and pseudoplastic debris flows, with lesser occurrences of subaqueous deposits, characterizing a proximal fluvial system deposition. The upper portion is made up of gravelly-sandy sediments that include subaqueous, debris flows and pseudoplastic debris flows deposits. They suggest deposition in a more distal zone as indicated by the larger occurrence of subaqueous deposits and the presence, though rare, of downstream-accretion macroforms. Besides the climate control, deposition of the Barreiras Group was strongly influenced by the intraplate tectonism, which has been affecting the South America Platform since the Middle Miocene, when neotectonism began in Brazil.

Relatively few investigations have employed electrical methods in the submarine environment, which may be promising for mineral deposits or threatened by environmental problems. We have measured the electric field using both disk and bar electrodes in the sea water at three different levels: sea surface, seven meters deep, and sea bottom at a depth of ten meters, employing a 2 m spacing dipole-dipole array with 7 array spacings of investigation, and 13 values of frequencies at steps of (2N hertz, N = -2, -1, 0, 1, 2,.....10). The measurement allowed the analysis of the electric field as a function of frequency and spacing, and of the spectral induced polarization. Modelling and interpretation of the apparent resistivity yielded a good fit with previous drilling data. Analysis of the spectrum of the complex apparent resistivity and the comparison with equivalent circuits, provided information about the grain size, the mineral composition and the major induced polarization phenomenon occurring below the sea. Therefore the result of the present research show the feasibility of measuring the variation of seawater resistivity in situ, as well as the resistivity of sea bottom sediments.

U-Pb SHRIMP and Sm-Nd isotopic ages were determined for felsic metavolcanic rocks from the Silvânia Sequence and Jurubatuba Granite in the central part of the Brasília Belt. Zircon grains from a metavolcanic sample yielded 2115 ± 23 Ma and from the granite yielded 2089 ± 14 Ma, interpreted as crystallization ages of these rocks. Six metavolcanic samples of the Silvânia Sequence yielded a six-point whole-rock Sm-Nd isochron indicating a crystallization age of 2262 ± 110 Ma and positive epsilonNd(T) = +3.0 interpreted as a juvenile magmatic event. Nd isotopic analyses on samples from the Jurubatuba Granite have Paleoproterozoic T DM model ages between 2.30 and 2.42 Ga and epsilonNd(T) values vary between -0.22 and -0.58. The oldest T DM value refers to a sedimentary xenolith in the granite. These results suggest crystallization ages of Silvânia volcanics and Jurubatuba Granite are the first evidence of a ca. 2.14-2.08 juvenile magmatic event in the basement of the central part of the Brasília Belt that implies the presence of arc/suture hidden in reworked basement of the Brasília Belt.

We consider the question of relating extrinsic geometric characters of a smooth irreducible complex projective variety, which is invariant by a one-dimensional holomorphic foliation on a complex projective space, to geometric objects associated to the foliation.

We study the behavior of massless Dirac particles, i.e., solutions of the Dirac equation with m = 0 in the presence of an electromagnetic field. Our main result (Theorem 1) is that for purely real or imaginary fields any Huygens type (in Hadamard's sense) Dirac operators is equivalent to the free Dirac operator, equivalence given by changes of variables and multiplication (right and left) by nonzero functions.

Electrostatic phenomena in insulators have been known for the past four centuries, but many related questions are still unanswered, for instance: which are the charge-bearing species in an electrified organic polymer, how are the charges spatially distributed and which is the contribution of the electrically charged domains to the overall polymer properties? New scanning probe microscopies were recently introduced, and these are suitable for the mapping of electric potentials across a solid sample thus providing some answers for the previous questions. In this work, we report results obtained with two of these techniques: scanning electric potential (SEPM) and electric force microscopy (EFM). These results were associated to images acquired by using analytical electron microscopy (energy-loss spectroscopy imaging in the transmission electron microscope, ESI-TEM) for colloid polymer samples. Together, they show domains with excess electric charges (and potentials) extending up to hundreds of nanometers and formed by large clusters of cations or anions, reaching supramolecular dimensions. Domains with excess electric charge were also observed in thermoplastics as well as in silica, polyphosphate and titanium oxide particles. In the case of thermoplastics, the origin of the charges is tentatively assigned to their tribochemistry, oxidation followed by segregation or the Mawell-Wagner-Sillars and Costa Ribeiro effects.

The generator coordinate Hartree-Fock method is used to generate adapted Gaussian basis sets for the atoms from Li (Z=3) through Xe (Z=54). In this method the Griffin-Hill-Wheeler-Hartree-Fock equations are integrated through the integral discretization technique. The wave functions generated in this work are compared with the widely used Roothaan-Hartree-Fock wave functions of Clementi and Roetti (1974), and with other basis sets reported in the literature. For all atoms studied, the errors in our total energy values relatively to the numerical Hartree-Fock limits are always less than 7.426 mhartree.

The generation of poly-hydroxilated transient species during the photochemical treatment of phenol usually impedes the spectrophotmetric monitoring of its degradation process. Frequently, the appearance of compounds such as pyrocatechol, hydroquinone and benzoquinone produces serious spectral interference, which hinder the use of the classical univariate calibration process. In this work, the use of multivariate calibration is proposed to permit the spectrophotometric determination of phenol in the presence of these intermediates. Using 20 synthetic mixtures containing phenol and the interferents, a calibration model was developed by using a partial least square regression process (PLSR) and processing the absorbance signal between 180 and 300 nm. The model was validated by using 3 synthetic mixtures. In this operation, typical errors lower than 3% were observed. Close correlation between the results obtained by liquid chromatography and the proposed method was also observed.

Bioceramic composites were obtained from chitosan and hydroxyapatite pastes synthesized at physiological temperature according to two different syntheses approaches. Usual analytical techniques (X-ray diffraction analysis, Fourier transformed infrared spectroscopy, Thermo gravimetric analysis, Scanning electron microscopy, X-ray dispersive energy analysis and Porosimetry) were employed to characterize the resulting material. The aim of this investigation was to study the bioceramic properties of the pastes with non-decaying behavior from chitosan-hydroxyapatite composites. Chitosan, which also forms a water-insoluble gel in the presence of calcium ions, and has been reported to have pharmacologically beneficial effects on osteoconductivity, was added to the solid phase of the hydroxyapatite powder. The properties exhibited by the chitosan-hydroxyapatite composites were characteristic of bioceramics applied as bone substitutes. Hydroxyapatite contents ranging from 85 to 98% (w/w) resulted in suitable bioceramic composites for bone regeneration, since they showed a non-decaying behavior, good mechanical properties and suitable pore sizes.