RCAAP Repository

Amostras fecais de bovinos comprovadamente parasitados e outras de animais possuidores de pâncreas macroscopicamente normais (controle) foram submetidas à prova do filme. Os resultados mostraram que tanto os animais parasitados quanto aqueles do grupo controle comportaram-se de modo semelhante à prova do filme. Com isto, concluiu-se que o referido teste deve ser desconsiderado para os fins propostos de diagnóstico presuntivo de infecções por Eurytrema sp em bovinos.

The counting of age rings in scales method was employed to determine growth curve for Astyanax bimaculatus in Ibitinga Reservoir (21°46'S and 48°59’W), according to von Bertalanffy, equation. 50 specimes, caught from July 1989 to June 1990 were analysed. It was also determined that the best body region for scales lecture, according to WERDER and SOARES 18 (1984), was surrounding the pectoral fin (E region). Observed results showed: - for 8 age classes, mean total lengths were: 1 - 61.03 mm; 2 - 75.30 mm; 3 - 86.67 mm; 4 - 97.92 mm; 5 - 109.80 mm; 6 - 121.75 mm; 7 - 134.13 mm and 8 - 144.40 mm. - The general growth curve equation estimated from the obtained data is: ^Lt = 223,14 [1 – e -0,1027 (t + 1,8549) ]

The influence of BCG on the resistance of hamsters experimentally infected with Leptospira interrogans serovar pomona was investigated. Forty-eight young male hamsters were used as the follow: 23 of them have administered two intraperitoneal dosis of BCG with seven days of interval: the remaining 25 were treated with placebo (Souton solution) in the same way as in the BCG treatment. On the third day after the second BCG or placebo administration, each animal was inoculated with 0.5 ml of a suspension of L. pomona through the intraperitoneal route. The animals were observed for signs and symptoms of leptospirosis during a period of 21 days and those in the agonic state of disease were killed and their kidney and serum samples were taken for the demonstration of antibodies and leptospiroses, by means of serum agglutination test and by the direct culture of kidney fragments. All of the survivors were killed at the 21st day post infection and evaluated for antibodies an leptospires, as described above. For the BCG treated group, no death was recorded (0/23 or 0,00%) whereas in the control group 80% of deaths (20/25) were observed.

Determinou-se a prevalência do nematóide Trichosomoides crassicauda na colônia convencional de ratos Wistar do Biotério Central (DTAEP) da Faculdade de Medicina da USP. Foram examinadas as bexigas urinárias de 365 ratos sendo 130 machos e 133 fêmeas, de 8 a 19 semanas de idade; e 51 machos e 51 fêmeas, com idade acima de 20 semanas, retirados da reprodução. Foi também determinada a prevalência de cálculos urinários, os quais têm sido associados à presença do parasita. Os resultados obtidos indicaram que a prevalência do T. crassicauda na colônia foi de 48% (43% em machos e 53% em fêmeas), sendo que o máximo valor observado foi 80%, em machos de 16 semanas de idade. O número médio de parasitas por rato não ultrapassou o limite de 2,5 em fêmeas de 16 semanas de idade. Não houve diferença na prevalência de cálculos urinários entre animais parasitados e não parasitados (10% e 16%, respectivamente), mas esta diferença foi significante entre machos e fêmeas (23% e 4%). Além disso, os cálculos encontrados nos machos (mucóides) foram diferentes daqueles encontrados nas fêmeas (cristais).

Fourteen thousand, one Hundred sixthy two slaughtered horses from nine Brazilian states at the FAVA S/A slaughter-house, Araguari, MG; between May, 14th/1987 to May, 10th/1988 and 31.49% had been Gasterophilus positive. During this period were observed positive animals from 7 different states: Mato Grosso do Sul (85.71%); São Paulo (75.95%); Paraná (65.61%); Goiás (44.02%); Mato Grosso (42.03%); Minas Gerais (14.03%); Pará (8.33%) and negative animals from 2 other states: Espírito Santo and Rio de Janeiro. Animals from 226 cities had been studied and 77.43% were gasterophilosis positive. The identified species was Gasterophilus nasalis.

Napier grass (Pennisetum purpureum Schum) in-3 growing stages: a) 1,30 m tall and 17.54% dry matter; b) 2.35 m tall and 25.84% dry matter: and c) 3,25 m tall and 26.31% dry matter, was fed "ad libitum" to eight rumen fistulated steers all crossbred (5/8 european-zebu) weighting 220 kg, being fed 1,5 kg of a concentrate meal with 24.16% of crude protein. Treatments were compared in a completely randonmized design using the 4th week of each experimental period for rumen liquor sampling for differencial counts of the following protozoa: Entodinium, Diplodinium, Eudipiodinium and Polyplastron. Results showed Entodinium as 96.73% of total protozoa and its concentration per mililiter of rumen fluid decreased linearly as the plant matured (a = 18.2 x 104/ml; b = 12.1 x 104/ml; and c = 9.8 x 104/ml).

A case report of gastroesophageal intussusception in a three-month-old German Shepherd bitch, a rare condition in this species, is presented. The animal had clinical signs of regurgitation and severe dyspnea and the diagnosis was made through thoracic radiography After fluidotherapy a surgical treatment was indicated. The laparotomy was performed, the stomach reduced to its normal anatomic position, and a fundoplication was done. Although the animal showed improvement of clinical signs, it died by the 7th day after surgery. The authors discuss about the propable etiologies of this condition, the possibility of a surgical treatment and the relationship between gastroesophageal intussusception and other esophageal pathologies

O quemodectoma é uma neoplasia derivada dos corpos aórticos e carotídeos, tendo origem neuroectodérmica. Tem sido relatado com maior freqüência em humanos e cães. Destes, os animais braquicefálicos são os mais susceptíveis, notadamente em faixa etária superior a 6 anos. O presente relato se refere a um animal de espécie canina, de raça Boxer, macho, de 7 anos de idade, apresentando histórico de quadro convulsivo e tosse seca. O animal veio a óbito e foi encaminhado ao setor de Anatomia Patológica do Departamento de Patologia da FMVZ-USP. O exame macroscópico evidenciou a presença de hemopericárdio, congestão e edema pulmonares, congestão hepática, renal e edema de vesícula biliar. Porém, o achado macroscópico mais significativo foi a presença de 4 formações tumorais ovaladas na cavidade torácica. O exame histopatológico das formações evidenciou um tecido sólido, altamente celular, com a delimitação de lóbulos através de trabéculas conjuntivas espessas, que emitiam septos delicados subdividindo-os. As células tumorais apresentaram-se uniformes, tendo um formato poliédrico, com núcleo grande esférico, geralmente excêntrico; nucléolo evidente, e cromatina finamente granular; citoplasma de dimensões variáveis e granulação acidofílica delicada. Foi observado pequeno número de figuras mitóticas sem sinais de atipia. Os quadros macro e microscópicos são compatíveis com o diagnóstico de quemodectoma.

Em 52 éguas de diferentes raças e idades, praticou-se cirurgia corretiva de dilaceraçâo perineal de 3º grau, com modificações na técnica de GÖTZE, na tentativa de restabelecer a anatomia do sistema reprodutor, bem como a vida reprodutiva destas fêmeas. Das éguas que sofreram a cirurgia, a totalidade (100%) retornou à reprodução, alcançando-se o índice máximo de fertilidade após inseminação artificial ou cobertura. Os resultados obtidos pelo emprego da técnica proposta permite indicá-la como uma conduta correta e eficiente na recuperação anátomo-funcional do genital de éguas com dilaceraçâo de períneo de 3º grau.

Foram utilizados 10 cães adultos, de ambos os sexos, sem raça definida, com peso corpóreo entre 10 e 18 kg. Os animais foram pré-medicados com 1,0 mg/kg de levomepromazina e 15 minutos após receberam 2,0 mg/kg de midazolam, ambos por via intravenosa. A freqüência respiratória mostrou um aumento moderado e a freqüência cardíaca redução aos 15 minutos e elevação aos 30 minutos. Houve redução da pressão arterial média e da pressão sistólica com significado estatístico e não clínico. Houve tendência de redução da pressão arterial diastólica. A hipnose durou aproximadamente 20 minutos e aos 30 minutos os cães estavam acordados e em tentativa de deambulação.

Com o objetivo de avaliar a possibilidade de associação e aplicação do cloridrato de quetamina e cloridrato de clorpromazina, por via intramuscular em cães, foi idealizado o presente experimento. Foram utilizados 10 animais adultos, de ambos os sexos, sem raça definida, com peso médio de 17 kg. A cada cão foi administrado 1 mg/kg de peso corpóreo de clorpromazina associado, numa mesma seringa, a 15 mg/kg de quetamina e aplicado por via intramuscular profunda. Foram avaliadas as freqüências cárdio-respiratórias, temperatura retal, valores/hemogasométricos, glicemia e traçados eletrocardiográficos, sendo os valores numéricos tratados pelo teste de Tuckey a 5% de probabilidade. Constatou-se aumento significativo da freqüência cardíaca, além de constantes supra e infradesnivelamento do segmento ST quando da análise eletrocardiográfica, sendo os demais valores de pouca relevância estatística. Concluiu-se que a associação pode ser empregada em pacientes hígidos, mas pressupõem-se cuidados quando do uso em cães portadores de cardiopatias.

O tumor de Ehrlich cresce na forma sólida quando inoculado no coxim plantar de camundongos, disseminando-se para o linfonodo poplíteo. Este estudo foi realizado com o intuito de caracterizar as células tumorais que cresceram no coxim plantar e as que migraram para o linfonodo poplíteo. O volume nuclear dessas células foi medido com o auxílio de ocular cariométrica. A quantificação do DNA foi feita através de citofotometria em células coradas pelo método de Feulgen. As células tumorais encontradas nos linfonodos poplíteos uma hora após a inoculação no coxim plantar não apresentaram diferenças estatísticas do tumor ascítico original e das células tumorais do coxim plantar em relação à quantidade de DNA, no mesmo tempo experimental. As células tumorais dos linfonodos poplíteos aos 30 dias após a inoculação mostraram menor volume nuclear e a mesma quantidade de DNA do que as que cresceram no coxim plantar. As células tumorais presentes no coxim plantar 30 dias após a inoculação mostraram uma quantidade maior de DNA do que as colhidas uma hora após a inoculação. Estes resultados sugerem um possível efeito seletivo nas células do tumor de Ehrlich que crescem no coxim plantar, mas não quando de sua metastatização para o linfonodo regional.

For the diagnosis of superficial mycoses, the clinical aspects and cultivation of fungi for further identification are usually considered. Identification, however, is time consuming and at least 4 weeks are needed for a positive laboratory report. In Brazil, the use of Wood’s light is not a routine screen test, especially in Veterinary Medicine. Thus, the present paper has the aim to evaluate the availability, the sensitivity and the specificity of the Wood's light in detecting fungal diseases because not all dermatomycoses are readily detected by this method. The fluorescence results were compared to those of fungi cultures of hair specimens from animals suspected of dermatophytes or yeast infections. The culture media used were the Sabouraud and the Mycose I Agar. From February 1989 to April 1990 the Dermatology Service of the University Veterinary Teaching Hospital attended 282 animals presenting lesions very suggestive of fungal diseases. From these, 162 (57.4%) were dogs and the remaining 120 (42.5%) were cats, of both sexes and of various ages and breeds. After performing dermatologie examination, the lesions were submitted to the Wood's light (250 nm) and fluorescence was observed in 70 (24.5%) cases. After the inoculation and in vitro cultivation of clinical specimens from either fluorescent and non-fluorescent lesions, positive fungal growth was found in 109 cases, 103 of these identified as dermatophytes or pathogenic yeast. The clear fluorescence was found only in Microsporum canis infection. The results of fluorescence and fungal growth were in accordance in 64.5% of the cases. The efficiency of the Wood's light in dogs and cats, compared to the mycological examination was respectively: sensitivity - 39.1% and 73.8%; specificity - 89.2% and 80.7%; possibility of diagnosis - 82.0% and 78.0%; predictable value of positive test - 37.5% and 67.3%; and predictable value of negative test - 89.8% and 85.1%. Epidemiologic aspects of dermalophytoses and yeast infections and fungi species isolated in cultures are furthermore discussed.

The anatomical and radiographical aspects of the urinary bladder of dogs were studied by cystography performed by excretory urography or urethrocystography. The dogs. clinicaly healthy, were divided in males and females groups. In both groups the piriform shape was the most frequent and in less frequence the oval shape was observed in both sex. No significant diference was observed in the position of the male e female urinary bladder. The mean distance between the urinary bladder with the contrast and the pubis margin was 0.65 cm in the males and 0.48 cm in the females. In all animals the superficial mucosa was smooth in all extension of the bladder wall, and the filling of the bladder lumen with a homogenous aspect of its cavity by the contrast.

Following previous studies in cistography technics, as well the anatomical and radiographycs aspects of the urinary bladder, we used this technic in 226 dogs with urinary bladder diseases referred to the Radiology Section over a 4 year period. We observed in a higher frequency irregularity of the mucosal surface due to chronic inflamation of the bladder, with no sex prevalence. In a lower frequency, the caudal dislocations of the urinary bladder are noted exclusively in the males. The cranial dislocations were seen more in males. The radiopaque calculus are more frequent in females. The bladder ruptures were seen in the males only. The ventral dislocation were more frequent in females and, finally in only two cases the failure in the filling of the urinary were observed in females.

Foram efetuadas contagens de ovos de Eurytrema sp em fezes de bovinos naturalmente infectados e os resultados indicaram que o método de exame coproparasitológico empregado, independentemente da carga parasitária do hospedeiro, tem 94,2% de probabilidade de diagnosticar casos de infecção, quando se efetua um único exame. Ademais, ficou comprovado que o número de ovos de Eurytrema sp por grama de fezes obedece a um modelo de distribuição binomial negativa e se caracteriza pela sua pequena magnitude.

This paper describes the fecundity and spawning behaviour of Astyanax bimaculatus, in Ibitinga Reservoir (21°46'S - 48°59'W), São Paulo State, collected from June 1989 to May 1990. 39 mature females were examined and the average fecundity resulted 12276 ± 8483 ovocytes. For determination of spawning behaviour 368 females were used. It was observed that the spawning is discontinuous, with annual periodicity and higher frequency  in December-January. The fecundity is a linear positive function of the ovary weight.

The arterial blood supply of 30 pairs of swines testicles were studied. The animals 6 to 10 months old were obtained from the “Campus Administrativo de Pirassununga, Faculdade de Medicina Veterinária e Zootecnia - USP” and from “Seara Industrial de Xanxerê S/A - Santa Catarina”. The testicular artery was injected with vinyl in order to obtain arterial models. In 50% of the preparations the testicular artery showed a variable number of lateral and medial branches; in 31.7% there was predominance of lateral branches. On the remaining 18.3% it was observed predominance of the medial branches. In 43.3% the testicular artery originated two branches, a lateral and a medial. In 30.0% of the preparations there was predominance of the medial branch, while the lateral one predominated on 13.3%. On the remaining 6.7% of the organs the testicular artery gave a dorsal branch and a ventral branch, the later prevailing on the vascularization of the organ. Concerning to the number and distribution of the arterial penetrant vessels, as analysed by the median method, it was possible to observe little differences among quadrants on the various models studied. When the testicular artery originated a variable number of lateral and medial branches (50%), it was possible to observe the presence of penetrant vessels that starting from the branching point reach oposite quadrants to the principal destination of the original artery. In 20.0% of the preparations when the division resulted in a lateral branch and a medial one, there were additional branches originating from the testicular artery prior to its division point. In 59 models (95.0%) anastomosis between the testicular artery and its main branches of theirs collaterals were identified. Those anastomosis were observed under thin albuginea and in the caudal face of the organ generally. Other anastomosis were noted between branches of the testicular and epididymary arteries. There were no significant differences among median values (P>0,05) of total number of penetrant vessels of the right or the left testicles.

The systematization of the biliary ducts of the liver of the great anteater (Myrmecophaga tridactyla) is analyzed. The ductus choledocus has no affluents and results from the union of the ductus cysticus and the ductus hepaticus always arises from theramus principalis dexter and the ramus principalis sinister, and is seen without tributaries in three cases, receiving them meanwhile in the other one. The ramus principalis dexter is made up of the ramus lateralis lobi dextri and the ramus medialis lobi dextri. The ramus principalis sinister is formed by the ramus lateralis lobi sinistri, the ramus lobi sinistri and the ramus lobi quadrati.

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