RCAAP Repository

The necropsy of 24 dogs with positive blood serum test for Dipetalonema sp microfilarias demonstrated that 93.9% of the parasites were located in the subcutaneous tissue and 6.1% in general visceral organs. The helminths were localized in different anatomical regions, the chest was found with the highest frequency.

Estudou-se a constituição da veia porta em bovinos da raça Nelore. Utilizaram nesse trabalho 30 fetos de bovinos, os quais tiveram seus sistemas da veia porta (extra-hepático) injetados com látex Neoprene 650 corado e dissecados. Os resultados do trabalho mostram variações no arranjo das raízes da veia porta e dos afluentes que compõem estes troncos. A disposição mais freqüente é a veia porta formada pelas veias esplênica, gastroduodenal e mesentérica cranial.

The distribution of the ramus principalis sinister of lhe biliary ducts of 40 adult females of Jaffarabadi breed buffaloes was studied after latex Neoprenie 650 and Celobar injections through theductus choledochus. Following fixation in 10% formaldehyde solution, radiograms were taken from the specimens and then dissected. The ramus principalis sinister is formed usually by several roots, ramus medialis lobi sinistri, truncus intermediomedialis, truncus intermediolateralis, ramus lateralis lobi sinistri. In addition, innominate tributaries originating from the lobus caudatus (pars supraportalis), lobus sinister (medial, intermediate and lateral territories), lobus quadratus and lobus dexter. In a few cases the ramus principalis sinister had components inter-related or related to the vesica fellea.

Testing 13 serotypes of Leptospira biflexa as antigens, for the diagnosis of leptospirosis in sheeps and goats by means of the microscopic agglutination test, it was observed that the antigens jequitaia and buenos aires presented the best results. If for sheep sera there was no significant difference between the antigens jequitaia and buenos aires (0,70 < p < 0,50), for goat sera the jequitaia antigen was better than buenos aires (0.01 < p < 0.002). The occurence of some false positive results showed limitations in the specificity of the SAT with Leptospira biflexa strains as antigens.

O exame da eficiência de seis vacinas antiaftosa, realizado com os testes “Indice Proteção Camundongos” e "Índice C” utilizando 15 ou mais cobaias por diluição de vírus na titulação, demonstrou a presença de boa correlação e alta significância entre os resultados das duas provas. O valor do índice Proteção Camundongos foi aproximadamente o dobro do valor do índice C. Assim, o índice Proteção Camundongos pode ser recomendado como teste de eficiência de vacinas antiaftosa. principalmente quando se necessita um grande número de provas, devido a seu baixo custo e facilidade de execução, por usar exclusivamente camundongos.

In veterinary dermatology, domestic carnivores sporadically are found with ulcerated lesions characterized by a prolonged course of evolution and by relative unresponsiveness to the usual drug therapy; this condition can be due to infections caused by microorganisms of the Genus Mycobacterium. Due to the scarcity of its description in Brazilian literature, and due to the inusitate lesions and response to preconized therapy, two cases of canine mycobacteriosis attended at the University Veterinary Teaching Hospital of the FMVZ/USP are then described. The adult bitches (German Shepherd and Doberman Pinscher) were found with ulcerated lesions at the posterior side of the pinna w ith granulomatous aspect and with exudation and covered by hemorrhagic crusts without showing any detectable satellite lymphadenomegalia or any symptoms in other systemic organs. Physical, hematologic and radiographic examinations were performed and lesions have been curetted and biopsied through punching technique, and specimens were examined histologically using Holst-Mitchinson and Radhakrishna and Ziehl-Neelsen staining, and throught culture methods using Lowenstein-Jensen and Stonebrink & Leslie media at different temperature of incubation. Intradermal tuberculin test using PPD tuberculin of mammalian and avian origin were performed. Presence of alcohol-acid resistant bacilli were detected throught histopathologic examination. The growth of bacilli, however, were not confirmed by the use of conventional media, even being incubated at different temperatures, although found with typical pyogranulomatous tissue reactions. After introducing the topical therapy, represented by the Domeboro’s solution, rifamycin. and parenteral use of rifampicin, lesions showed marked remission. Etiology, pathogeny and other diagnostic and therapeutic maneuvers are also discussed.

The main objective of this investigation was to evaluate the improvement in skin wound healing in cats with the use of soft laser (diodes) Ga-As-SHLD radiation (904 nm). Ovariohisterectomised cats were divided into a control group and a laser-treated group. Anatomopathological observations were taken on days 2, 4 and 8, post-surgery along with macroscopic evaluation. We concluded that laser diode Ga-As-SHLD application ameliorated the surgical wound healing process by increasing its tensile strength.

Seminal characteristics of six donkeys of the "Pega" breed, ranging from 3 to 9 years of age were studied. Between October 1988 and April 1989, seventy (70) ejaculates were collected by using artificial vagina, twice a week. The following average values, for the parameters studied were found: total volume (59,88 ± 15.54 ml), gel volume (8,19 ± 4.07 ml), total spermatozoal motility (81,93 ± 6,27%), progressive motility (72,86 ± 7,20%), sperm vigor (4,63 ± 0,49), sperm concentration (444,11 ± 182,72 x 10³/mm³), total sperm number per ejaculate (24,88 ± 9,59 x 10 9), total abnormal cells (15,60 ± 3,62%), major defects (8,55 ± 2,14%) and minor defects (7,04 ± 2,56%). These results were quite similar to that reported in the literature for donkeys. Compared with the values found by other authors lor stallions, both species are very similar, differing only in some aspects. The total volume of jackass ejaculate was inferior, however, since the gel occurrence was much lower than in stallions, the "sperm-rich" fraction volume was approximately the same. The sperm concentration and total sperm number per ejaculate were higher for asses. Furthermore, the parameters of spermatozoal motility and vigor demonstrated that jackass semen was, almost always, of superior quality. In artificial insemination programmes, these characteristics would indicate the possibility of higher number of individual-doses per ejaculate and better freezability levels.

This work studied DM, CP and CF ruminal degradability, through nylon-bag technique "in situ", of Napier grass (Pennisetum purpureum Schum) on several growth stages. Eight rumen fistulated steers were used to compare 4 treatments: A = 72 days and 1,30 m high; B = 105 days and 2,35 m high; C = 135 days and 3,25 m high and D = 161 days and 3,25 m high, considering age and stand of grass regrowth. Experimental design was completely randonmized, and the results showed that DM, CP and CF rumen degradabilities decreased linearly with grass growth (p < 0,01). Degradability values for 48 h incubation time were DM: 56,02%; 47,71%; 43,57% and 38,76%; CF = 47,37%, 41,7%, 32,28% and 22,92% for A, B, C and D treatments. Concerning CP, only A, B and C stages were analyzed: with the following results: 53,59%, 35,32% and 26,42% for 48 h of incubation time. Rumen volume increased linearly with grass age (p <0,01) and rumen turn-over was higher for A (p < 0,05).

Estudou-se em 30 cães SRD, fetos e natimortos, a origem, o número e a ordenação dos vasos tímicos utilizando-se da dissecção de peças injetadas com solução de Neoprene látex 450 e fixadas em solução de formol a 10%. Os resultados permitiram concluir que a irrigação do timo é feita por ramos diretos e indiretos oriundos das artérias torácicas internas, tronco braquiocefálico, artérias pericardicofrênicas, troncos costocervicais e artérias subclávias, que combinadas entre si, emitiam de cinco a catorze contribuições, que por sua vez evidenciaram modalidades de vascularização própria para cada peça.

The biliary tree of two adult, male, tapirs (Tapirus americanus) was injected with latex. After the fixation of the liver with 10% formaldehyde solution the specimens were dissected and photographed. The ductus eloledocus is formed by the confluence of the ramus principalis dexter and sinister, since this species does not possess a vesica fellea. The ramus principalis dexter is made up of the ramus ventralis lobi dextri, ramus medius lobi dextri, ramus dorsalis lobi dextri and ramus processi caudati, which join each other under several arrangements. The same occur with the ramus principalis sinister, formed by the ramus medius lobi sinistri lateralis, ramus dorsalis lobi sinistri lateralis, ramus lobi quadrati, ramus ventralis lobi sinistri lateralis and ramus lobi sinistri medialis.

The alpha2-macroglobulin (alpha2M) protease inhibitor was purified from bovine plasma. The alpha2M preparations at various purification steps were identified by immunodiffusion and crossed immunoelectrophoresis with anti-human alpha2 serum. Anti-bovine alpha2M serum was prepared for the quantitative determinations. The purest alpha2M preparation was obtained by affinity chromatography and used as primary standard in radial immunodiffusion. Alpha2M preparations were submitted to binding tests with p' - NPGB (p‘ - nitrophenyl-p-guanidinebenzoate HCL) titrated trypsin and plasmin. Alpha2M protected 35% of the esterolytic activity of trypsin and 50% of the amidolytic activity of plasmin.

It were studied 60 hearts of crossbreed pigs to observe the frequency, width and localization of the myocardial bridges on the coronary arteries. The hearts had their coronary arteries injected with colored solution of Neoprene latex - 450 - and were fixed in formalin solution to posterior dissection. The results permited the following conclusions: -myocardial bridges occured in 36.36% of the examined hearts in number between one and three in the same hearts and it had been found a total number of 32 bridges: -the myocardial bridges width varied from 0.2 cm to 1.9 cm with an average of 0.75 cm: -myocardial bridges are more frequent in the medium (36.66%), dorsal (31.25%) and ventral (31.25%) portions of the studied hearts; -myocardial bridges occur predominantly on the right coronary artery branches (65.62%) than on the left coronary artery branches (34.37%).

Os autores esquematizam as vias biliferas de 2 espécies de tatus (Dasypus novemcinctus e Euphractus sexcinctus) oriundos do Pantanal Matogrossense, Brasil. A dissecação foi efetuada após o preenchimento do sistema excretor do fígado com látex Neoprene 650 corado e da fixação das peças em solução aquosa de formol a 10%. O ductus choledocus, em ambas as espécies, provém da reunião do ductus cysticus com o ductus hepaticus. Este, por sua vez, resulta da convergência do ramus principalis dexter e do ramus principalis sinister. Na formação dos ramus principalis dexter e sinister participam vários ramos oriundos dos lobos hepáticos e seus processos.

The constitution of the ductus biliferus principalis dexter, the ductus choledocus, the ductus cysticus and the vesica  fellea were studied anatomically and radiologically in 40 Jaffarabadi, adult buffaloes, after latex Neoprene and Celobar injection of the biliary tract. The ductus choledocus did not have tributaries (97.5%), except in one case (2.5%), in which it received a tributary from the lobus quadratus. The choledochus was formed by the confluence of the ductus biliferus principalis dexter et ductus biliferus principalis sinister in 75% of the cases, (dorsomedial and ventrolateral roots). It was formed by the ductus biliferus principalis sinister (22.5%) and by the junction of the ductus hapaticus and the ductus cysticus in 2.5%. The ductus cysticus is a constant component of the ductus principalis dexter. It joins the ductus lateralis lobi dextri (67.5%). The vesica fellea does not show tributaries in 87.5% but in 12.5% receives hepatocystic ducts from the lobus quadratus (5%), from the lobus dexter and the lobus quadratus (5%) and from only the lateral territory of the lobus dexter (5%). The ductus biliferus principalis dexter is formed by the ductus lateralis lobi dextri and ductus cysticus (100%) and by the ductus medialis lobi dextri, ductus dorsalis and ductus processi caudati (92.5%).

Treze bezerros 3/4 holandês x 1/4 zebu, nascidos em diferentes estações do ano de 1989 e criados intensivamente, foram transferidos para pastagens de capim gordura (Melinis minutiflora) entre 7 meses e 1 ano de idade, no final do período chuvoso (março de 1990), a fim de ser acompanhado o curso natural da eliminação de ovos de Eurytrema sp por grama de fezes (OPG). De 15 em 15 dias, foram feitas as colheitas de fezes para obter as contagens de OPG durante 18 meses. Os primeiros animais que apresentaram exames positivos (a partir de agosto de 1990) foram os que tiveram as maiores contagens de OPG durante o período experimental. Os demais apresentaram os primeiros exames positivos em diferentes ocasiões e as contagens de OPG destes foram baixas e oscilantes. Três animais apresentaram exames negativos. Provavelmente, a maioria dos bovinos criados em regime de pastejo desta região deverão apresentar infecção parasitária por Eurytrema sp em alguma fase de suas vidas.

Descreve-se a tuberculose desencadeada pela inoculação intraperitoneal de 1.0 mg de Mycobacterium bovis (cepa AN 5) em hamsters através de exames bacteriológicos e histopatológicos, realizados 1, 15, 29 e 45 dias após a infecção. A bactéria se multiplicou no baço, rins, fígado e pulmões dos animais infectados, induzindo a formação de lesões granulomatosas. Um dia após a infecção o baço albergou um maior número de bactérias e 45 dias após a infecção houve diferença nas concentrações bacterianas apenas entre baço e rim, com um maior número de bactérias para o baço. Foram observadas duas fases de bacteremia e a micobactéria foi cultivada a partir do líquido peritoneal em todas as etapas do experimento. Os animais perderam peso em decorrência da doença e morreram em média 50.6 dias após a infecção.

Males and females of Crotalus durissus terrificus which were born in captivity were used to test artificial insemination techniques. The diluted sperm was introduced into the vagina and after 120 days the first 3 snakes were dissected while in other group of snakes, pregnancy was allowed to run its natural course. All the snakes of the latter group gave birth to live and normal young and no atresic eggs were observed.

Seminal samples of six jackass of Pega breed were analysed for pH (n=70), osmolality (n=69) and Na (n=49), K (n=51), Ca(n=51) and Mg (n=51) levels determinations. The pH values ranged from 7.0 to 7.8 with the average of 7.29± 1.18 for the total ejaculate number. Seminal plasma osmolality stayed between 273 and 373 mOsm, with the total average of 302.89± 15.03 mOsm, which represents a freezing point depression of 0.56°C. The meamconcentrations of Na, K, Ca and Mg in the seminal plasma were, respectively, 238.26±67.92,1 05.20±32.14,13.24± 1.62 and 1.54±0.10 mg%. All the result obtained at the present study were compared to scientific information about donkeys and horses. Both species are quite semilar apart from Mg levels that was lower for the donkeys. Due to the similarities between jackass and stallion seminal characteristics we believe that procedures for equine semen “in vitro” conservation might also be used for jackass semen. Complementary research is need to confirm the procedures viability.

The kinetic of vascular and cellular alterations in the carrageenin-induced peritonitis was investigated (500 mcg) in Gallus gallus and the effect of the pre-treatment with dexamethasone (0.5; 1.0 or 2.0 mg/kg), indomethacin (2.0; 4.0 or 8.0 mg/kg), pyroxiean (20.0; 40.0 or 80.0 mg/kg), given by oral route 30 minutes before the inflammatory stimulus. It was observed that the highest increase in both vascular permeability and leucocyte accumulation ocurred respectively 150 minutes and 4 hours after the carrageenin injection. Polymorphonuclear leukocytes were the predominant cell type in the exudate obtained 2 and 4 hours after the stimulus. An equilibriun between polymorphonuclear and mononuclear cells was observed with 24 hours of inflammation and the latter cell type was predominant after 48 hours. The pre-treatment with indomethacin and pyroxiean significantly (p<0.05) inhibited the vascular permeability increase but not the leukocytes counts. Dexamethasone was significantly reduced both vascular permeability increase and leukocyte accumulation. These results suggest that eicosanoids have a role in the vascular permeability increase but it is less relevant in the leuchocyte chemotaxis in carrageenin-induced acute inflammation in fowls. The vascular permeability increase and cellular migration are independent phenomena when the effect of antiinflammatory drugs are concerned.