RCAAP Repository

Evidence for ancient interspecific gene flow through hybridization has been reported in many animal and plant taxa based on genetic markers. The study of genomic patterns of closely related species with allopatric distributions allows the assessment of the relative importance of vicariant isolating events and past gene flow. Here, we investigated the role of gene flow in the evolutionary history of four closely related freshwater fish species with currently allopatric distributions in western Iberian rivers—Squalius carolitertii, S. pyrenaicus, S. torgalensis and S. aradensis—using a population genomics dataset of 23,562 SNPs from 48 individuals, obtained through genotyping by sequencing (GBS). We uncovered a species tree with two well-differentiated clades: (i) S. carolitertii and S. pyrenaicus; and (ii) S. torgalensis and S. aradensis. By using D-statistics and demographic modelling based on the site frequency spectrum, comparing alternative demographic scenarios of hybrid origin, secondary contact and isolation, we found that the S. pyrenaicus North lineage is likely the result of an ancient hybridization event between S. carolitertii (contributing ~84%) and S. pyrenaicus South lineage (contributing ~16%), consistent with a hybrid speciation scenario. Furthermore, in the hybrid lineage, we identify outlier loci potentially affected by selection favouring genes from each parental lineage at different genomic regions. Our results suggest that ancient hybridization can affect speciation and that freshwater fish species currently in allopatry are useful to study these processes.

Background: Recent studies have revealed the benefits of using colchicine, a drug with anti-inflammatory properties, in coronary artery disease (CAD). This study systematically reviewed the benefits and risks of low-dose colchicine in patients with CAD. Methods and Results: We searched for randomized controlled trials (RCTs) in MEDLINE, the Cochrane Central Register of Controlled Trials (CENTRAL), and Web of Science databases (March 2020). Efficacy and safety outcomes were evaluated. Estimates are expressed as risk ratios (RRs) and 95% confidence intervals (95% CIs). Heterogeneity was assessed with I2 test. Confidence in the pooled evidence was appraised using the GRADE framework. Colchicine reduced the rate of major adverse cardiovascular events (RR 0.65; 95% CI 0.49-0.86; 6 RCTs; I2=50%; 11,718 patients; GRADE, moderate confidence), acute coronary syndrome (RR 0.64; 95% CI 0.46-0.90; I2=47%; 7 RCTs; 11,955 patients; GRADE, very low confidence), stroke (RR 0.49; 95% CI 0.30-0.78; I2=0%; 6 RCTs; 11,896 patients; GRADE, moderate confidence), and cardiovascular interventions (RR 0.61; 95% CI 0.42-0.89; I2=40%; 4 RCTs; 11,284 patients; GRADE, high confidence). Colchicine did not increase the risk of adverse events, except for gastrointestinal events (RR 1.54; 95% CI 1.11-2.13; I2=72%; 9 RCTs; 12,374 patients; GRADE, very low confidence). Conclusions: Low-dose colchicine in patients with CAD is associated with beneficial effects on prognosis, although an increased risk of gastrointestinal events was confirmed.

TRALI é uma reacção transfusional grave associada à transfusão de componentes sanguíneos. É caracterizado por insuficiência respiratória aguda, edema pulmonar bilateral e hipoxia severa, sem comprometimento cardíaco, ocorrendo durante ou dentro das 6 horas, após a transfusão de um ou mais componentes sanguíneos. Foi demonstrado que uma parte dos casos de TRALI é devida a anticorpos contra antigénios de neutrófilos humanos (HNA) ou contra antigénios de leucócitos humanos (HLA) de classe I e de classe II. Os anticorpos anti-HLA no dador são formados pela exposição a células ou tecidos de outro ser humano (alo-exposição). Esta alo-exposição pode ser provocada por gravidez, transfusão de componentes sanguíneos ou transplante de stem cells, tecidos ou órgãos. Contudo nem sempre a alo exposição termina com a formação de anticorpos (alo-imunização). Partindo do pressuposto que todos os dadores que tenham sido transfundidos são eliminados da dádiva só nos resta a aloexposição por gravidez. Assim a pesquisa de anticorpos anti-HLA classe I e classe II foram realizadas a um grupo de dadores de sangue, do sexo feminino com história prévia de gravidez, do Centro Regional de sangue de Lisboa. Das 400 dadoras estudadas podemos concluir que cerca de 12% apresentam HLA classe I, 12% apresenta HLA classe II e 3% apresentam HLA classe I e classe II. O total de aloimunizações neste grupo de estudo ronda os 21%. A frequência de anticorpos anti-HLA classe II para o caso de dadoras com três ou mais gestações é bastante maior comparada com o caso de dadoras com uma ou duas gestações, embora possamos concluir que no caso das dadoras em que as gestações levaram a aborto, pelo reduzido tempo de exposição não levou à imunização induzida por anticorpos anti-HLA. Como conclusão podemos aplicar as medidas, implementadas por outros países, para redução dos casos de TRALI que passa por reduzir o uso de PFC das dadoras do sexo feminino, dar preferência aos dadores do sexo masculino no caso de colheita de componentes sanguíneos por aférese e a pesquisa de anticorpos anti-HLA a todas as dadoras de primeira vez e sempre que sejam aloexpostas.

The depletion and overexploitation of several fish stock demands for a valorisation of non-target and discarded species. Nonetheless, such species are often poorly studied, and information on their biological parameters must be gathered for effective population management. For 1 year, the reproductive strategy of the piper gurnard Trigla lyra and the red gurnard Chelidonichthys cuculus was studied by monthly samples obtained from commercial boats operating on western Portuguese coast. Both species showed a biased sex ratio towards females, especially for larger length classes. Length at first maturity could be estimated only for red gurnard (22.1 and 19.9 cm for females and males, respectively) because all piper gurnard individuals caught were mature. Piper gurnard showed determinate fecundity and a short spawning season, from November to February with a peak in January, whereas red gurnard showed indeterminate fecundity and a wide spawning season, from late December to May. The relative annual fecundity estimated for red gurnard (1893 ± 728 oocytes × g−1 eviscerated weight [EW]) was higher than the one estimated for piper gurnard (1018 ± 250 oocytes×g−1 EW). Although important information for understanding the species dynamics is presented in this study, additional information on other life-history parameters and of species landings is required.

This article examines the relations between Portuguese society and Salazar’s political police (PIDE) from the perspective of the everyday lives of ordinary citizens – in contrast to the small minority of oppositionists that has so far monopolized the attention of historians. It is based on a quantitative survey of 400 respondents in four separate locations across Portugal and addresses two main research questions: To what extent did the sample of ordinary citizens experience the PIDE as a disruptive influence on their daily lives? Was the PIDE ‘normalized’ by them as part of the framework of everyday life? The data analysis calls upon the inputs of the international bibliography of everyday life under dictatorship and critically engages with the existing historiography of the PIDE.

During the years of economic crisis and austerity, and the subsequent economic growth dependent on real estate and tourism, housing has returned into the spotlight on the political agenda in Southern European countries and cities, where activists and social movements scaled up their struggles and created bridges with institutional actors, fostering policy change. The latter, however, did not happen in Italy. In this article, based on exploratory case study research carried out in the city of Turin, we present three themes that help explain what we call the ‘absent politicization’ of housing in Italy during the last decade: a multi-actor, multilevel housing policy capable of defusing specific problems; the absence of bridges between politicized and institutional actors; and the role played by party-politics, with attention to ‘populist’ Movimento 5 Stelle in power in Turin. By focusing on differences with Southern Europe, we contribute to overcoming dichotomies that have long dominated comparative housing studies; and contribute to linking housing studies with contentious urban politics in the post-crisis years.

In this article—based on the fieldwork I conducted in Lisbon (Portugal) between 2018 and 2021, employing in-depth ethnography and self-ethnography—I describe the experience of the medicalization and moralization of beauty in Portuguese women aged 45–65 years. I examine the ways in which practitioners inscribe their expert knowledge on their patients’ bodies, stigmatizing the marks of time and proposing medical treatments and surgeries to “repair” and “correct” them. Beauty and youth are symbolically constructed in medical discourse as visual markers of health, an adequate lifestyle, a strong character and good personal choices (such as not smoking, and a healthy diet and exercise habits). What beauty means within the discourse of anti-aging and therapeutic rejuvenation is increasingly connected to an ideal gender performance of normative, white, middle-class, heterosexual femininity that dismisses structural determinants. The fantasy of eternal youth, linked to a neoliberal ideology of limitless enhancement and individual responsibility, is firmly entrenched in moralizing definitions of aesthetics and gender norms. Finally, my article highlights the ways in which the women I interviewed do not always passively accept the discourse of the devaluation of the ageing body, defining femininity and ageing in their own terms by creating personal variants of the hegemonic normative discourses on beauty and successful ageing.

Atrial fibrillation (AF), the most common arrhythmia in the adult population worldwide, represents a significant burden in terms of cardiovascular mortality and morbidity and has repercussions on health economics. Oral anticoagulation (OAC) is key to stroke prevention in AF and, in recent years, results from landmark clinical trials of non-vitamin K oral anticoagulants (NOAC) have triggered a paradigm shift in thrombocardiology. Despite these advances, there is still a significant residual vascular risk associated with silent AF, bleeding, premature sudden death and heart failure. The authors review AF epidemiologic data, the importance of new tools for early AF detection, the current role of catheter ablation for rhythm control in AF, the state-of-the-art in periprocedural OAC, the optimal management of major bleeding, the causes of residual premature death and future strategies for improvements in AF prognosis.

Mucormycosis is an uncommon, life-threatening infection caused by opportunistic fungi included in the Mucorales order, which mostly affects immunosuppressed patients. Mucormycosis can exhibit different clinical presentations depending on the involved organ, including pulmonary, gastrointestinal, rhinocerebral, cutaneous, and disseminated infections.

Com a transição democrática iniciada em Portugal em 1974, também a polícia procurou organizar-se em sindicatos. Mas esta aspiração só foi alcançada no início do século xxi, quando se abriu caminho à proliferação sindical, tendo-se atingido as 19 organizações em 2019. Este artigo mostra que a instrumentalização da forma legal pelos polícias levou ao desvirtuamento da natureza da ação sindical. Com base em entrevistas e na análise documental, conclui-se que o sistema de aferição de representatividade sindical deve implicar critérios objetivos e previsíveis para que haja condições mínimas (não suficientes) para o diálogo social.

We test for integration of financial markets in China during 1920-1933 using a new dataset of domestic exchange rates. Our data concerns tael-denominated telegraphic transfers between Shanghai and nine other cities. We find that Chinese financial markets, as measured by the efficiency of silver-point arbitrage, were highly integrated among major commercial hubs in north and central China, but there was a lower level of integration for more remote cities in the south. Our paper presents the first comprehensive assessment of the efficiency of the Chinese silver standard and contributes to a revaluation of market performance during pre-communist China.

Study question: What are the data on ART and IUI cycles, and fertility preservation (FP) interventions reported in 2017 as compared to previous years, as well as the main trends over the years? Summary answer: The 21st ESHRE report on ART and IUI shows the continual increase in reported treatment cycle numbers in Europe, with a decrease in the proportion of transfers with more than one embryo causing an additional slight reduction of multiple delivery rates (DR) as well as higher pregnancy rates (PR) and DR after frozen embryo replacement (FER) compared to fresh IVF and ICSI cycles, while the number of IUI cycles increased and their outcomes remained stable. What is known already: Since 1997, ART aggregated data generated by national registries, clinics or professional societies have been gathered and analyzed by the European IVF-monitoring Consortium (EIM) and communicated in a total of 20 manuscripts published in Human Reproduction and Human Reproduction Open. Study design size duration: Data on European medically assisted reproduction (MAR) are collected by EIM for ESHRE on a yearly basis. The data on treatments performed between 1 January and 31 December 2017 in 39 European countries were provided by either National Registries or registries based on personal initiatives of medical associations and scientific organizations. Participants/materials setting methods: Overall, 1382 clinics offering ART services in 39 countries reported a total of 940 503 treatment cycles, including 165 379 with IVF, 391 379 with ICSI, 271 476 with FER, 37 303 with preimplantation genetic testing (PGT), 69 378 with egg donation (ED), 378 with IVM of oocytes, and 5210 cycles with frozen oocyte replacement (FOR). A total of 1273 institutions reported data on 207 196 IUI cycles using either husband/partner's semen (IUI-H; n = 155 794) or donor semen (IUI-D; n = 51 402) in 30 countries and 25 countries, respectively. Thirteen countries reported 18 888 interventions for FP, including oocyte, ovarian tissue, semen and testicular tissue banking in pre- and postpubertal patients. Main results and the role of chance: In 21 countries (20 in 2016) in which all ART clinics reported to the registry, 473 733 treatment cycles were registered for a total population of approximately 330 million inhabitants, allowing a best-estimate of a mean of 1435 cycles performed per million inhabitants (range: 723-3286).Amongst the 39 reporting countries, the clinical PR per aspiration and per transfer in 2017 were similar to those observed in 2016 (26.8% and 34.6% vs 28.0% and 34.8%, respectively). After ICSI the corresponding rates were also similar to those achieved in 2016 (24% and 33.5% vs 25% and 33.2% in 2016). When freeze all cycles were removed, the clinical PRs per aspiration were 30.8% and 27.5% for IVF and ICSI, respectively.After FER with embryos originating from own eggs the PR per thawing was 30.2%, which is comparable to 30.9% in 2016, and with embryos originating from donated eggs it was 41.1% (41% in 2016). After ED the PR per fresh embryo transfer was 49.2% (49.4% in 2016) and per FOR 43.3% (43.6% in 2016).In IVF and ICSI together, the trend towards the transfer of fewer embryos continues with the transfer of 1, 2, 3 and ≥4 embryos in 46.0%, 49.2%, 4.5% and in 0.3% of all treatments, respectively (corresponding to 41.5%, 51.9%. 6.2% and 0.4% in 2016). This resulted in a reduced proportion of twin DRs of 14.2% (14.9% in 2016) and stable triplet DR of 0.3%. Treatments with FER in 2017 resulted in a twin and triplet DR of 11.2% and 0.2%, respectively (vs 11.9% and 0.2% in 2016).After IUI, the DRs remained similar at 8.7% after IUI-H (8.9% in 2016) and at 12.4% after IUI-D (12.4.0% in 2016). Twin and triplet DRs after IUI-H were 8.1% and 0.3%, respectively (in 2016: 8.8% and 0.3%) and 6.9% and 0.2% after IUI-D (in 2016: 7.7% and 0.4%). Amongst 18 888 FP interventions in 13 countries, cryopreservation of ejaculated sperm (n = 11 112 vs 7877 from 11 countries in 2016) and of oocytes (n = 6588 vs 4907 from eight countries in 2016) were the most frequently reported. Limitations reasons for caution: As the methods of data collection and levels of reporting vary amongst European countries, interpretation of results should remain cautious. Some countries were unable to deliver data about the number of initiated cycles and deliveries. Wider implications of the findings: The 21st ESHRE report on ART, IUI and FP interventions shows a continuous increase of reported treatment numbers and MAR-derived livebirths in Europe. Being already the largest data collection on MAR in Europe, efforts should continue to optimize data collection and reporting with the perspective of improved quality control, transparency and vigilance in the field of reproductive medicine. Study funding/competing interests: The study has received no external funding and all costs are covered by ESHRE. There are no competing interests.

Recentemente, surgiram novas propostas de explicação para o crescimento de raízes que sugerem que estas apresentam uma forma de inteligência colectiva, proveniente dos comportamentos simples dos ápices que se baseiam em informações locais. A esta propriedade, é dado o nome de Swarm Intelligence. Com o objectivo de verificar a viabilidade de explicar o crescimento das raízes utilizando modelos de inteligência colectiva, no que se refere à exploração que efectuam do solo, foi criado um modelo do crescimento de raízes no solo discreto, programado em Java e acessível através de uma página de Internet. Este incorpora processos de escolha distribuídos que são realizados pelos ápices, que decidem, baseados em informação local, que nutriente explorar, se irão ramificar-se e em que direcção do solo crescer. O solo faz também parte do modelo, sendo composto por vários elementos cúbicos, dispostos num paralelepípedo. Cada um pode conter uma quantidade variável de água, azoto e fósforo, apresentando entre si processos de difusão simples. A raiz interage com o solo através do processo de uptake, que consiste no processo de aquisição de nutrientes do solo pela raiz. O modelo é controlado através de uma interface gráfica que apresenta um variado leque de sliders e checkboxes que permitem controlar os vários parâmetros envolvidos no crescimento dos ápices e apresenta como resultado a arquitectura tridimensional da raiz, passível de ser visualizada de várias formas. Com a observação das simulações realizadas através do modelo, verificou-se que as características gerais da exploração realizada por raízes eram replicadas por este. Concluiu-se, portanto, que, apesar de algumas limitações do modelo, a explicação das estratégias de exploração do solo pela raiz à luz de uma forma primitiva de inteligência é, efectivamente, viável.

Transthyretin amyloid cardiomyopathy (ATTR-CM) is a life-threatening condition with a heterogeneous clinical presentation. The recent availability of treatment for ATTR-CM has stimulated increased awareness of the disease and patient identification. Stratification of patients with ATTR-CM is critical for optimal management and treatment; however, monitoring disease progression is challenging and currently lacks best-practice guidance. In this report, experts with experience in treating amyloidosis and ATTR-CM developed consensus recommendations for monitoring the course of patients with ATTR-CM and proposed meaningful thresholds and frequency for specific parameters. A set of 11 measurable features across three separate domains were evaluated: (i) clinical and functional endpoints, (ii) biomarkers and laboratory markers, and (iii) imaging and electrocardiographic parameters. Experts recommended that one marker from each of the three domains provides the minimum requirements for assessing disease progression. Assessment of cardiac disease status should be part of a multiparametric evaluation in which progression, stability or improvement of other involved systems in transthyretin amyloidosis should also be considered. Additional data from placebo arms of clinical trials and future studies assessing ATTR-CM will help to elucidate, refine and define these and other measurements.

The World Heart Federation (WHF) commenced a Roadmap initiative in 2015 to reduce the global burden of cardiovascular disease and resultant burgeoning of healthcare costs. Roadmaps provide a blueprint for implementation of priority solutions for the principal cardiovascular diseases leading to death and disability. Atrial fibrillation (AF) is one of these conditions and is an increasing problem due to ageing of the world's population and an increase in cardiovascular risk factors that predispose to AF. The goal of the AF roadmap was to provide guidance on priority interventions that are feasible in multiple countries, and to identify roadblocks and potential strategies to overcome them. Since publication of the AF Roadmap in 2017, there have been many technological advances including devices and artificial intelligence for identification and prediction of unknown AF, better methods to achieve rhythm control, and widespread uptake of smartphones and apps that could facilitate new approaches to healthcare delivery and increasing community AF awareness. In addition, the World Health Organisation added the non-vitamin K antagonist oral anticoagulants (NOACs) to the Essential Medicines List, making it possible to increase advocacy for their widespread adoption as therapy to prevent stroke. These advances motivated the WHF to commission a 2020 AF Roadmap update. Three years after the original Roadmap publication, the identified barriers and solutions were judged still relevant, and progress has been slow. This 2020 Roadmap update reviews the significant changes since 2017 and identifies priority areas for achieving the goals of reducing death and disability related to AF, particularly targeted at low-middle income countries. These include advocacy to increase appreciation of the scope of the problem; plugging gaps in guideline management and prevention through physician education, increasing patient health literacy, and novel ways to increase access to integrated healthcare including mHealth and digital transformations; and greater emphasis on achieving practical solutions to national and regional entrenched barriers. Despite the advances reviewed in this update, the task will not be easy, but the health rewards of implementing solutions that are both innovative and practical will be great.

The writing of history, in medieval Latin, in the territory todayknown as Portugal, has been poorly studied. For the periodbefore 1200, critical editions, comprehensive studies, even a listof known texts are lacking. Further, there has been no attempt tounderstand the Latin historiography of Portugal in its properIberian context. This paper seeks to address these issues.Focusing on the lands that would, during the twelfth century,evolve from the county of Portucale into the kingdom of Portugalup to the beginning of the thirteenth century, the paperestablishes the historiographic sub-genres employed in theperiod; identifies the texts that were held in north-westernIberian libraries as possible sources or models for new texts; andpresents an annotated catalogue of all known historiographicaltexts produced in the region.

It is a major challenge in clinical epilepsy to diagnose and treat a disease characterized by infrequent seizures based on patient or caregiver reports and limited duration clinical testing. The poor reliability of self-reported seizure diaries for many people with epilepsy is well-established, but these records remain necessary in clinical care and therapeutic studies. A number of wearable devices have emerged, which may be capable of detecting seizures, recording seizure data, and alerting caregivers. Developments in non-invasive wearable sensors to measure accelerometry, photoplethysmography (PPG), electrodermal activity (EDA), electromyography (EMG), and other signals outside of the traditional clinical environment may be able to identify seizure-related changes. Non-invasive scalp electroencephalography (EEG) and minimally invasive subscalp EEG may allow direct measurement of seizure activity. However, significant network and computational infrastructure is needed for continuous, secure transmission of data. The large volume of data acquired by these devices necessitates computer-assisted review and detection to reduce the burden on human reviewers. Furthermore, user acceptability of such devices must be a paramount consideration to ensure adherence with long-term device use. Such devices can identify tonic-clonic seizures, but identification of other seizure semiologies with non-EEG wearables is an ongoing challenge. Identification of electrographic seizures with subscalp EEG systems has recently been demonstrated over long (>6 month) durations, and this shows promise for accurate, objective seizure records. While the ability to detect and forecast seizures from ambulatory intracranial EEG is established, invasive devices may not be acceptable for many individuals with epilepsy. Recent studies show promising results for probabilistic forecasts of seizure risk from long-term wearable devices and electronic diaries of self-reported seizures. There may also be predictive value in individuals' symptoms, mood, and cognitive performance. However, seizure forecasting requires perpetual use of a device for monitoring, increasing the importance of the system's acceptability to users. Furthermore, long-term studies with concurrent EEG confirmation are lacking currently. This review describes the current evidence and challenges in the use of minimally and non-invasive devices for long-term epilepsy monitoring, the essential components in remote monitoring systems, and explores the feasibility to detect and forecast impending seizures via long-term use of these systems.

The current COVID-19 pandemic has challenged health systems and communities globally. As such, several countries have embarked on national COVID-19 vaccination programmes in order to curb spread of the disease. However, at present, there isn’t yet enough dosages to enable vaccination of the general population. Different vaccine prioritization strategies are thus being implemented in different communities in order to permit for a systematic vaccination of individuals. Here, on behalf of the World Heart Federation, we emphasize the need for individuals with Cardiovascular disease to be prioritized in national vaccine prioritization programmes as these are high risk individuals.

Apresenta-se uma síntese dos conhecimentos sobre as manifestações epigráficas associadas à antiga escrita que se desenvolveu no Sudoeste hispânico na I Idade do Ferro. Abordam-se alguns aspectos fundamentais, começando por uma breve história da investigação, a que se juntam os tópicos relativos ao sistema de signos, à natureza dos monumentos, aos seus textos e contextos. Apesar dos novos achados das últimas décadas e dos trabalhos arqueológicos em sítios associados às estelas inscritas, são ainda muitas as dificuldades inerentes a estes temas e, por isso, muitas das questões encontram-se em aberto.

A Hispânia pré-romana apresenta-se culturalmente muito diversa, em certos aspetos mesmo contrastante. Essa multiplicidade cultural reflete-se igualmente no domínio linguístico. Ao invés de uma antiga tradição que postulava uma língua única originária, o quadro na altura da conquista apresenta uma compartimentação básica entre línguas indo-europeias (em que sobressaem o celtibérico e o lusitano) e as que não pertencem a este grupo (onde se destaca o ibérico). A realidade é, no entanto, mais complexa e as perspetivas sobre ela são muito diversas. Pretende-se dar conta, de uma forma necessariamente muito sintética, da evolução da investigação e do estado atual dos nossos conhecimentos neste domínio.