RCAAP Repository

NOGUEIRA, Vanessa Lúcia Rodrigues. Papel de proteínas na tinta liberada pela lesma-do-mar Aplysia dactylomela Rang 1828 os mecanismos de defesa do animal. 2005. 90 f. Dissertação (Mestrado em Ciências Marinhas Tropicais) - Instituto de Ciências do Mar, Universidade Federal do Ceará, Fortaleza, 2005.

The Schistosomiasis is endemic in 76 countries and territories. In mid 2003, it was estimated that 779 million people were within the population at risk for schistosomiasis, 207 million people were infected. The laboratory diagnosis of schistosomiasis can be accomplished through methods for parasites, ranging from classics (Kato-Katz), a few that are still undergoing validation. This study was conducted to evaluate two coproscopic new methods for diagnosis of Schistosomiasis in residents of an area of low endemicity in Maranguape-Ceará, using the Kato-Katz as a reference and serology (ELISA) for screening of patients. We developed the following steps: knowing the area and dissemination of the project with residents in the locality; Home visit to signing the consent form and interview to collect epidemiologic data, blood collection for performing the serological method, distribution of the bottles for feces, only for participants who were reactive in serological testing, collection of stool samples and carrying out the methods coproscopic and finally, delivery of results of examinations and treatment of positive individuals. Comparing the results obtained by the Kato-Katz method and salt gradient was seen that the 13 positive (23.2%) found by both, 10 (76.9%) were diagnosed only by the saline gradient. Even when we compared the method of Kato-Katz and Helmintex of the 32 samples analyzed, 16 were positive (50%) for both, however, 12 (75%) of these individuals were diagnosed only by Helmintex. And when comparing the method the saline gradient with Helmintex in 32 individuals who completed these, 17 (53%) were positive by both techniques, 11 (64.7%) positive only in Helmintex. Thus, the methods of the Saline Gradiente and Helmintex were more effective in the diagnosis of schistosomiasis in locus study, when compared to the Kato-Katz, but on the way of implementing the techniques, they may not be suitable for large surveys epidemiological, but for specific studies in areas where the driver fails to achieve the goals.

Background: Beta thalassemia is a group of disorders, each resulting from a genetic defect in the rate of synthesis of one or more globin chains of hemoglobin (Hb). The imbalance in the production of globin chains can result in ineffective erythropoiesis, insufficient production of hemoglobin, hemolysis and anemia of varying degree. Beta thalassemia is more common in countries bordering the Mediterranean Sea, reflecting the participation of these peoples in the formation of the Brazilian population. The predominant mutations in Brazil are the IVS-I-1, IVS-I-6, IVS-I-110 and CD 39, which are associated with different clinical conditions and are mostly regionally specific. Objective: To characterize the clinical, hematological and molecular adults with beta thalassemia of the University Hospital Cantídeo Walter and followed at a referral center for Hematology of the state of Ceará (Hemoce). Methods: We analyzed 22 individuals with beta thalassemia, 7 intermediate and 15 minor, in both sexes, from February 2008 to September 2009. Clinical data and laboratory tests: blood count, levels of Hb A2 and Hb F, serum iron, total capacity and latent iron binding (CTLFe, CLLFe), ferritin and transferrin saturation index (IST) were obtained from medical records, diagnosis. About 5 mL of venous blood was collected in tubes containing EDTA anticoagulant for molecular study. The analysis of mutations was performed using the technique of chain reaction mediated by allele specific polymerase (PCR-AE), where we analyzed the following mutations: IVS-I-1, IVS-I-6, IVS-I-110 and CD 39. Statistical analysis was carried out in software R (version 2.7.0) and the level of significance was 5%. Results: Of 22 patients studied, 15 were patients with β thalassemia minor and seven intermediate. The age ranged 18-68 years with a mean of 44.7 years. 18.2% male and 81.8% female. The mutations were characterized in 68.2% of cases, which had the most frequent IVS-I-6, followed by the codon 39. The mutation IVS-I-1 was found in one patient and IVS-I-110 was not found. There was no significant clinical differences between the hematological and biochemical parameters. There was a discrepancy between phenotype and genotype in some patients, but no significant difference between mutations and clinical manifestations. Conclusions: The results of this study reinforce the dominance of the mutation IVS-I-6 in northeastern Brazil. Are recommended further studies to investigate the co-inheritance with α-thalassemia in these patients to justify the discrepancy between genotypes and phenotypes.

Hodgkin’s lymphoma is a entity where free disease and overal survive is prolonged by treatment and its molecular basis have been studied. In previus literature is described p53 as a protein that controls progress of celular cicle and TP 53 gene mutations prolong half-life of protein, modifing the mechanisms of apoptosis and regulation of celular proliferation. About of 50% of human cancers have TP53 mutations and the investigators believe that in the others the signaling mechanisms of p53 are altered by others ways. Hodgkin’s lymphoma cells stain for p53 frequently, but detection for TP53 gene mutation is rare. It’s not be well stabilished if p53 is a prognostic factor in Hodgkin’s lymphoma. There is no trial about p53 expression on Hodgkin’s lymphoma in Ceará state. Estrogen receptor were detected by imuno-histochemical analysis in normal bone marrow cells and others non-hematopoietic tissues. There is evidence that estrogen have a role on differentiation of B-cells and on regulation of apoptosis. If is Hodgkin’s lymphoma(HL) a B-cell neoplasm, with immune response seems to contribute in its pathogeny, its interesting to study the role of estrogen on Hodgkin’s lymphoma and its influence on this neoplasm behavior. We studied p53 and estrogen receptor expression in Hodgkin’s lymphoma on Ceará state. Were studied 39 cases of Hodgkin’s lymphoma confirmed by histo-pathological studies and by immuno-histochemical analysis and a control group with 10 cases of benign linfonodal hyperplasia. The simples were stained with p53 and estrogen receptor(ER) α anti-bodies. The age of patitens varied between 5 and 63 years, 21% were males, the initial presentation of disease was cervical adenopathy on 59% of cases and B simptoms were present on 56% of patients. On classification by histological subtipes, 56% were classical HL nodular sclerosis, 27% mixed cellularity, 14% lymphocyte-rich and 3% were HL linfocyte predominance. In this sample, lymphocyte-depleted subtype was not found. Positive staining for p53 occurred on 53% of cases and 10% of control group. Estrogen receptor was negative in all cases of Hodgkin’s lymphoma, but showed positive stainning in neutrophilic cells. In control group was founded ER positivity in 80% of cases, in histiocytic cells, with para-nuclear stainning and cytoplasmic diffuse. In conclusion, p53 expression occurs in more than 50% of Hodgkin’s lymphoma cases, as described previously, and there is expression of ERα in lymphoid tissue, but not on HL. There is persistent positivity in neutrophil cytoplasma. Another investigations are necessary to avaliate ERα and ERβ on RS cells and their role on the pathogenesis of Hodgkin’s lymphoma.

Apical periodontitis, most frequent as a consequence of dental caries, is considered a pathological process of difficult control and cure, due to the involvement of immune system in its evolution and maintenance. The low-level laser therapy (LLLT) is lately being used as a therapeutic measure in some areas of Dentistry; however its mode of action and the consequent effects on apical periodontitis are not yet well understood. The objectives of the present study was to show a histological evaluation of the consequence of application of LLLT during the evolution of experimentally induced inflammatory apical lesion in rats, and evaluate the effect of use of LLLT as an auxiliary therapy to conventional endodontic treatment of established apical periodontitis, in the same experimental model. The study was performed in two stages: in the first phase, 40 adults, male Wistar rats were utilized, with four animals used as “sham-operated” control group (GC), and the other 36 animals divided into two groups of 18 each (G1 and G2), and subjected to induction of apical periodontitis by opening the crown of the left front molar and leaving the root pulp open to the oral cavity. The animals of G1 suffered only the induction of lesion, while those of G2 also received LLLT, at 830nm wave length and exit potency of 100 mW in the dental apical region, on days 0, 10, 20 and 30 (total irradiation dose of 28 J/cm2, at 5.6 J/cm2 per application). The animals were sacrificed in the following manner: one animal of the control group (GC) on each of the days 0, 10, 20 and 30, and six animals from each of the groups G1 and G2, on days 10, 20 and 30. In the second phase of the study, 12 animals constituted the following three experimental groups of four each (G3, G4 and G5): G3 – apical periodontitis induced and sacrificed 21 days later; G4 – apical periodontitis induced + treated with Calen® on day 21 of induction of lesion, and sacrificed 21 days later; and G5 – apical periodontitis + treated with Calen® + LLLT applied as in G2, and sacrificed 21 days later. Histological evaluations performed on the left mandibles revealed that animals of the control group (GC) did not have alterations in the periapical tissues. All the animals of G1 developed apical periodontitis, while the G2 animals treated with LLLT from day 0 of lesion induction revealed markedly more intense inflammatory lesions and greater alveolar bone loss in the periapical region, as compared to those of G1 which did not receive laser irradiation. Histological studies on the second phase groups G3, G4 and G5, demonstrated that animals treated with Calen® alone (G4), and with Calen® + LLLT (G5) presented less severe apical periodontitis on day 42 of induction of disease, when compared with those of G3 at day 21 of disease induction. More significantly, animals of G5, treated also with LLLT, revealed less intense apical periodontitis, than those of G4 which received only Calen® treatment. These evidences indicate that low-level laser irradiation, when employed as the only treatment for apical periodontitis, could present the risk of aggravating an ongoing inflammatory process; while its use as an adjunct therapy to conventional endodontic treatment seems to contribute to a better control of the periapical inflammatory disease. These preliminary, and apparently contradictory, results seem to suggest that LLLT may not, in fact, possess an anti-inflammatory potential attributed to it in occasional reports, but it likely exerts some stimulatory influence on the activity of immune cells engaged in the inflammatory disease process, or on those involved in the resolution of the inflammatory disease initiated by the conventional endodontic therapeutic measures.

CASTRO, Otavio Serino. Efeito da fonte de óleo da dieta sobre o desempenho zootécnico, perfil lipídico e características sensoriais do camarão Litopenaeus vannamei cultivado em condições de alta salinidade. 2010. 96 f. Dissertação (Mestrado em Ciencias Marinhas Tropicais) - Instituto de Ciências do Mar, Universidade Federal do Ceará, Fortaleza, 2010.

TORRES, Roseline Freire. Disponibilidade dos metais cobre e chumbo em um canal de maré receptor de efluentes de carcinicultura. 2009. 125 f. Dissertação (Mestrado em Ciências Marinhas Tropicais) - Instituto de Ciências do Mar, Universidade Federal do Ceará, Fortaleza, 2009.

PITOMBEIRA, Sheila Cavalcante,. Zoneamneto ecológico-econômico do Estado do Ceará : instrumento jurídico de proteção da zona costeira do Ceará. 2007. 128 f. Dissertação (Mestrado em Ciências Marinhas Tropicais) - Instituto de Ciências do Mar, Universidade Federal do Ceará, Fortaleza, 2007.

A lagosta Panulirus argus (Latreille) constitui o principal recurso pesqueiro do nordeste brasileiro, sendo largamente explorada. No presente trabalho procuramos determinar o tamanho mínimo de captura para a lagosta Panulirus argus, tendo em vista tornar máxima sua produção no Estado do Ceará (Brasil).

TELLES, Francisco José Siqueira. Enterobactérias nas águas estuarinas do Rio Jaguaribe (Ceará). Arquivos de Ciências do Mar. Fortaleza, v. 15, n.2, p. 97-99, dez. 1975.

ARAÚJO, Genésio Alves de et al. Redução do óxido de trimetilamina por bactérias. Arquivos de Ciências do Mar. Fortaleza, v. 15, n.2, p. 101-103, dez. 1975.

TELLES, F. J. S.; VIEIRA, R. H. S. dos F.; Vieira, G. H. F. Industrialização de pescadas marinhas e de águas doces.: II - Processamento em forma de produto enlatado. Arquivos de Ciências do Mar. Fortaleza, v. 15, n.2, p. 111-113, dez. 1975.

FAUSTO FILHO, Jose. Sobre a ocorrência de Squilla grenadensis Manning, 1969 no litoral brasileiro (Stomatopoda, Squillidae). Arquivos de Ciências do Mar. Fortaleza, v. 15, n.2, p. 133-135, dez. 1975.

ZAPATA, Jorge Fernando Fuentes; MAGALHÃES NETO, Esmerino de Oliveira. Industrialização de pescadas marinhas e de águas doces.: l - Processamento em forma de produto defumado e pasta frita. Arquivos de Ciências do Mar. Fortaleza, v. 15, n.2, p. 105-110, dez. 1975.

MORAIS, Jader Onofre de; SOUSA, Marcos José Nogueira; COUTINHO, Paulo da Nóbrega. Contribuição ao estudo geomorfológico-sedimentológico do litoral de Beberibe (Ceará-Brasil). Arquivos de Ciências do Mar. Fortaleza, v.15, n.2, p.71-78, dez. 1975.

ALVES, Maria Ivone Mota. Sobre a reprodução do caranguejo-uçá, Ucides cordatus (Linnaeus), em mangues do Estado do Ceará (Brasil). Arquivos de Ciências do Mar. Fortaleza, v.15, n.2, p.85-91, dez. 1975.

PAIVA, Melquíades Pinto. Estudo sobre a pesca de lagostas no Ceará, durante o ano de 1974. Arquivos de Ciências do Mar. Fortaleza, v. 15, n.2, p. 115-117, dez. 1975.

PAIVA, Melquíades Pinto et al. Prospects for the world marine fishery production. Arquivos de Ciências do Mar. Fortaleza, v. 15, n.2, p. 125-126, dez. 1975.

FAUSTO FILHO, Jose. Quinta contribuição ao inventário dos crustáceos decápodes marinhos do Nordeste brasileiro. Arquivos de Ciências do Mar. Fortaleza, v.15, n.2, p.79-84, dez. 1975.

RODRIGUES, Maria Margarida. Alimentação do ariacó, Lutjanus synagris Linnaeus, do Estado do Ceará (Brasil). Arquivos de Ciências do Mar. Fortaleza, v. 14, n.1, p. 61-62, jun. 1974.