Repositório RCAAP

This study aimed to evaluate the effect of age and lineage of layers breeders light and semi heavy on the quality of the egg, the pinto newly eclodido and performance of incubation. 3,456 eggs were used for breeding light (Dekalb White) and semi heavy (Bovans Goldline) in three different ages (young, old and middle age). The experiment was divided into two stages, at first we evaluate the characteristics of the eggs of a sample of 360 eggs (60 per treatment). In the second stage evaluated the performance of chicks hatching and quality of a sample of 3,096 eggs (516 per treatment). It was observed that the quality of the shell fell and the weight of the egg increased with the increase of age of the birds. The layers breeders Dekalb White eggs produced heavier in the middle ages and with better quality of bark at all ages. With the increasing age of the bird has increased the percentage of yolk and albumen decreases. This decrease was more pronounced in a manner eggs line Dekalb White. Eggs of the same lineage, layers breeders young and old had a higher percentage of egg yolk. The pH of the yolk and albumen increased with the ageing of layers breeding, which in old age, the egg line Dekalb White shows higher values for these variables that line Bovans Goldline. As for the assessment of incubation found that birds' eggs old line Bovans Goldline had greater percentage of weight loss during the incubation than those from Dekalb White layers breeders of the same age. The weight of pinto increased with increasing age of the leyer breeding and Dekalb White chicks born heavier that the Bovans Goldline. However, the relationship of chicks weight / weight of the egg was higher for chicks Bovans Goldline poultry young and middle age. The progeny Dekalb White was not influenced by the age of reproductive on this parameter. The percentage of females born in respect of fertile eggs was greater in the lineage Dekalb White. Since young matrices obtained lower values for this variable, regardless of their lineage. The female chicks Dekalb White, and had weights more uniform, had a higher percentage of bag viteline than the Bovans Goldline. The age of the matrix no effect on these variables. The percentage of the heart and lungs in the weight of the female chicks were not influenced by age or the lineage of breeding.

Twenty-two castrated male pigs individually housed were used to compare two different genetics lineages (A and B) derived from Large White breed, submitted to different tools of genetic selection, considering: growth performance, intestinal morphometry, carcass and pork quality. In each of the two lineages, they were analised for: (1) Performance: weight, total feed intake, feed efficiency, carcass yield, viscera weight and intestinal morphometry; (2) Carcass quality: Water holding capacity, drip loss, color, intramuscular fat, muscular fibers morphometry (LD and SM muscles), sensorial analysis, and leptin (obese) gen. There were no statistical difference (p>0.05) between studied lineages, showing standard of rearing conditions. Divergent tools in genetic selection didn t influence performance growth, carcass and pork quality. Pork form both lineage were considered of great quality by consumer s test.

The present study aimed to evaluate the effects of the supplementation of different sources of protein concentrates, glutamine, acid glutamic and or yeast extract in diets of piglets from 26 to 44 days of age. At 22nd day of age, weaning day, 210 piglets, females and castrated males, crossbred of Landrace, Large White and Duroc races, with 6,79 kg average weight, had been classified in three groups according to weight. Until 25th day of age, all them received the same feed, without inclusion of yeast extract and/or glutamine e glutamic acid. In 26th day of age (zero day), the supply of the experimental diets was initiated as: T1: Pre-initial feed with yeast extract, gutamine and glutamic acid; T2: Preinitial feed with yeast extract; T3: Pre-initial feed with glutamine and glutamic acid. The amounts of additives were indicated by the manufacturers. The additives tested, yeast extract and glutamine e glutamic acid, were enclosed considering their nutritional profile. The experimental diets (Pre-initial I and Preinitial II) were constituted of maize pre-stew (or gelatinized), soybean meal, sugar, milk supplements, organics acids, soybean oil, vitamin-minerals mixtures and other additives. During all the period of the test, the feed intake was measured by pen. It was evaluated, in each period, average daily feed intake (ADFI), average daily weight gain (ADWG), feed conversion (FC), of protein utilization efficiency rate (PUE) and energy utilization efficiency rate (EUE) of each class of weight and treatment. Blood samples of three piglets from each pen at the end of supply of experimental diets (44 days of age) were collected to carry out hemogram and biochemist analyses (glucose, urea, creatinine, serum total proteins and albumin). The values of globulins had been calculated by the difference between serum total proteins and albumin concentrations. The relation albumin: globulin (A/G) was gotten by estimative, dividing albumin concentration by globulin concentration. In each pen, organs (spleen, stomach, liver, pancreas, kidneys, and intestine) were collected from one piglet, which live weight was next to average of group. Fragments of duodenum, jejune and ileum had been fixed to measure histomorphometric characteristics as: height of the villosities (HVILOS), depth of crypts (DCRIP), to estimate relation HVILOS/DCRIP, villosities and crypts width to calculate M value, which represents how many times intestinal mucosal surface was higher. Gotten data have been submitted to procedures of statistics program SISVAR. In statistics analysis, averages had been compared by Tukey test, at 5% of probability. For M value, data were submitted to Kolmogorov-Smirnov test to confirm normal distribution; after that, variance analisys was taken, and test t was applied in pairs of traits. Analysis of variance in randomized complete block designwas used, considering three treatments and three blocks (categories of weight). The yeast extract and or glutamina and acid glutamic use in diets of weaned pigs made possible the similar performance and physiological results between the treatments. The surface of intestinal absorption in the duodenum was bigger in the treatments contends yeast extract.

CHAPTER 2: Among the organic waste produced by poultry activity, the chicken manure presents potential to be used in the rehabilitation of degraded areas. The experiment was developed in RED LATOSSOL distrofic under grazing of Brachiaria decumbens in a state of advanced deterioration to evaluate the influence of fertilization and mineral with chicken manure in productivity, attributes bromatologics and in the absorption of nutrients from Brachiaria decumbens. The approach was mounted in completely randomized design, which were used six treatments (control without fertilization organic or mineral, control with mineral fertilization, four unique strengths of chicken manure (3,125, 6,250, 9,375 and 12,500 kg ha-1). The application of the residue the haul was in coverage, there were two collections the of Shoot of upland (December 2005 and February 2006). Observed that the influence of the application on productivity occurred only in 2 collection and was more expressive in dealing with 9,375 and 12,500 kg ha-1 of chicken manure. The behavior observed for CP, NDF ADF and lignin were little influenced by the organic fertilization or mineral, expecting that the crude protein were more influenced by fertilization depending on the content of nitrogen present in the chicken manure. The absorption of nutrients was more efficient for phosphorus and potassium, and was not significant for the calcium, magnesium, and sulfur, but it is recommended that sulfur is supplemented in another experiment with application of this waste. Concludes that the chicken manure presents potential to be used in pastures of Brachiaria decumbens, favoring its recovery function mainly in the levels of phosphorus and potassium. CHAPTER 3: The creation of turkey if has developed in Brazil mainly in the last decade, in virtue of the increase of the demand of the external and internal market. Despite the presented similarity the chicken manure enters and the turkey manure, is necessary to evaluate particularities of this residue in the recovery of an area degraded under pasture vegetation. The experiment was developed in a dystrophic RED LATOSSOLO under pasture of Brachiaria decumbens in advanced state of degradation to evaluate the influence of the mineral fertilization and the turkey manure in the chemical attributes of the ground, and in the absorption of nutrients and the bromatologic characteristics of the Brachiaria decumbens. The delineation was mounted in completely randomized design, where six treatments had been used (witness, a mineral dosage, four exclusive dosages of turkey manure (1,695; 3,390; 5,085 and 6,780 kg ha-1). The application of the residue was the throwing in covering, became fulfilled two collections leaf in the period of waters. It presents itself as an excellent organic residue to promote the recovery of degraded areas despite its bigger increments have been on levels of phosphorus and potassium. It was hoped that the turkey manure it is larger increments in productivity of DM and crude protein, which has not occurred, indicating the need for more research and use of this residue. CHAPTER 4: Objective to evaluate the influence of fertilization mineral and liquid swine slurry in productivity, bromatológic and absorption of nutrients from Brachiaria decumbens. The experiment was developed in a LATOSSOLO RED distrofic under grazing of Brachiaria decumbens, in a state of deterioration with evolutionary loss of vigor and productivity of forage, advanced. The approach was mounted in completely randomized design with six treatments (control without fertilization, control with the use of mineral fertilizer, four unique strengths of liquid swine slurry (60 m3 ha-1, 121 m3 ha-1, 181 m3 ha-1 and 241 m3 ha-1). The application of the residue was done to haul in coverage, took place two applications of waste separate and two collections of leaf material (December 2005 and February 2006). Noted that the liquid swine slurry promoted increases in productivity of dry matter and the green pasture. However not promoted any improvement in the absorption of nutrient evaluated. The liquid swine slurry also promoted reduction in the content of the forage ADF. It is recommended greater care in the characterization of excrements in experimentation, and conditions this experiment their application not caused environment impact to the system.

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Twenty four rabbits were separate in control group (GC) and treated with stem cell group (GCT). Under general anesthesia a Rosenthal 16G needle was inserted into umeral tubercle to collect 2,0mL of bone-marrow. It was proceeded the isolation, the counting and the viability of the mesenchymal autologous cells. A lateral parapatelar incision was made over the left knee in skin and articular capsule. The patella was medially dislocated and a segment about 0,4cm x 1,0cm was removed in the throclear groove. In GC and GCT that local was filled by alogenic osteochondral graft conserved in glycerin. In GCT it was inject 2 x 106 mononuclear stem cells. On 45 and 90 days of postoperative, in 75% of GC animals the articular capsule presented thick and in 41,66% adhered to adjacent tissues. The graft was osteointegrated in 33,33% and 58,33% with bone alterations. In GCT the graft adhered into the receptor bed, in 91,66% degeneration process was not observed, and in 58,33% it was noted endochondral ossification of the subchondral bone with bone consolidation. Alogenic osteochondral grafts conserved in 98% glycerin in rabbits throclear groove repair, associated with intra-articular injection of autologous mononuclear cells, stimulate fibrocartilage and hyaline cartilage formation with integration of the graft into the receptor bed.

Conselho Nacional de Desenvolvimento Científico e Tecnológico

The R. sanguineus is the tick with most world wide distribution and represents, as curiosity, the unique genus in Brazil. This acarus is associated to the presence of its host, the dog, and is used as a vector to agents of high importance diseases such as canine erliquiosis and babesiosis and humans botonose and spotted fever. The present paper had the aim to evaluate the efficiency and to characterize the induced immune answer by a mosquito subolesin recombinant vaccine and orhtolog of the tick Ixodes scapularis against R. sanguineus. In this evaluation six dogs were vaccine inoculated and other six were inoculated only with the adjuvant. The animals were then challenged with experimental infestation to evaluate vaccine s efficiency. The tick dog s cutaneous reaction was rated by histopathology. Hyper sensibility cutaneous test with tick antigens was done to estimate vaccinated dog s immune answer alterations. Antibodies titles against the vaccinal antigen were estimated by ELISA. Although there was a decrease in the recovering rate of grubs and nymphs in the vaccinated animals there wasn t statistical differences in biological parameters between the two experimental groups. The vaccinated animals presented more discreet hemorrhages, necrosis and edema at the tick s fixation point. The cutaneous hyper sensibility of vaccinated animals was exacerbated. The vaccinated animals presented an elevation in anti-subolesin antibodies. It s been concluded that the tested vaccine doesn t induce to a remarkable resistance to the tick R. sanguineus in spite of it can modifcate the hot s reaction.

The growing number of domestic cats (Felis catus) living in close relationship with humans, in the condition of animal companions, has led to a concomitantly increasing demand for new knowledge about the species, particularly about the hematological and serum biochemical parameters, which are important tools in the evaluation and monitoring of their health and their adaptation to the lifestyle imposed on them. In this context, the purpose of this study was to determine the physiological changes, and the influence of racial factors and of sex on the hematological and serum biochemical parameters of adult domestic Persian and half-breed cats. Analyses were made of the hematological parameters and 24 serum biochemical constituents of 106 adult domestic cats, over 24 months of age, males and females, 51 Persians and 55 half-breeds. With regard to the hematological parameters, it was found that the mean values of rod neutrophils of the half-breed cats and of eosinophils of both Persians and half-breeds were higher than the reference values for the species. The serum biochemical profile of the alanine aminotransferase (ALT) activity in the Persian cats and the concentration of urea in both Persians and half-breeds showed mean values exceeding the reference values reported in the literature. A comparison of the hemograms of the Persians and half-breeds revealed statistically significant differences in the values of their globular volume, mean globular volume (MGV), and number of basophils. As for the serum biochemistry, of the 24 elements analyzed, statistically significant differences were found in the mean values of very low density lipoproteins (VLDL), triglycerides, total proteins, albumin, globulins, ALT, alkaline phosphatase, phosphorus, Ca:P ratio, uric acid, and amylase between the Persian and half-breed cats. No significant difference was found between males and females in the values of the hematological of serum biochemical parameters analyzed. It was concluded that the breed, but not sex, influences the hematological parameters and the serum biochemical profile of adult domestic cats.

In this study, the origin and distribution of the axillary nerve in 30 fetuses of zebu-crossed bovines, 20 males and 10 females, were analyzed through dissection after fixation in an aqueous solution of 10% formaldehyde. The axillary nerve was found to originate in 13.33% of the antimeres of the ventral branch of the 6th cervical (C6) spinal nerve, in 100% of the antimeres of the ventral branch of the 7th cervical (C7) spinal nerve, in 91.67% of the ventral branch of the 8th cervical (C8) spinal nerve, and in 5.00% of the ventral branch of the 1st thoracic (T1) spinal nerve. The axillary nerve extended branches to the teres minor (100,00%), deltoid (100,00%), cleidobrachial part of the brachiocephalic muscle (100,00%), subscapularis (78.33%) and teres major (11.66%) muscles. In all the animals, the aforementioned nerve issued the cranial cutaneous branch of the lower leg, which was distributed to the fascia and skin of the cranial face of the upper and lower leg. No significant differences were found in the frequency of branches of the axillary nerves issuing to the muscles of the right and left antimeres. However, statistically significant differences were found in the frequency of branches leading to the right deltoid muscle in males and females, with male fetuses showing a higher frequency of these branches.

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The aim of this research was to evaluate the hypothesis of Uberlândia presenting LVC in two areas that represent a potential risk of arrival and dissemination of LV in this city, as well as clarifying the role of the kennel APA (ANINAL PROTECTION AGENCY) and the homes localized at the Tenda dos Morenos and Buracão for the epidemiology of LV in the city. For that reason, a sectioned study was done in these places. At APA, a sampling activity was done with 85 dogs, which were photographed, identified, evaluated for visual inspection and submitted to blood drawings with filter paper at the tip of the ear for the making of RIFI ou ELISA at the LACEN of FUNED. In 25 of these animals it was also collected blood drawings samples, through puncture in the vessels of thoracic/pelvic members, for the realization of PCR of blood at the Molecular Biology Laboratory at UFU. The fifty five dogs in the homes of the Tenda dos Morenos and Buracão region were identified, evaluated by visual inspection and submitted to blood drawing sampling at the tip of the ear with filter paper and were sent to LACEN of FUNED from Belo Horizonte for the realization of the ELISA and RIFI tests. The animals reactive to some of these tests were re-tested with ELISA or RIFI and also with PCR of blood. In spite of the clinical findings which were very compatible to the LVC at the APA, no positive animals were found for the illness in the investigated regions. Thus, the moment regarding attention to health issues for LV in these regions is of primordial prevention and primary prevention, in order to avoid the formation of the bond parasite-reservatory-vetor. Therefore, the conjugation of this serum tests along with molecular tests has shown to be of great importance so the canine monitoring can be satisfactory.

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This is study aimed to evaluate the performance and carcass characteristics in broilers fed exclusively with vegetal products and with diets included animal by-products added or not with creatine. Animal byproducts and creatine were used from eight days of age. It was used 2160 oneday- old male chicks distributed in a completely randomized design with twelve treatments and six replications. The diets were formulated based on corn and soybean meal according to treatments: TMT A. corn + soybean meal (CSM); TMT B. (CSM) + creatine; TMT C. (CSM) + meat and bone meal (MBM); TMT D. (CSM) + MBM + creatine; TMT E. (CSM) + blood meal (BM); TMT F. (CSM) + BM + creatine; TMT G. (CSM) + feather meal (FM); TMT H. (CSM) + FM + creatine; TMT I. (CSM) + viscera meal (VM); TMT J. (CSM) + VM + creatine; TMT K. (CSM) + MBM + FM + VM + BM; TMT L. (CSM) + MBM + FM + VM + BM + creatine. Performance, feed intake, live weight, feed conversion and viability were obtained at seven, 14, 21, 35 and 42 days of age. At 42 days of age, four birds per treatment were selected to evaluate the carcass yield (CY), boned breast yield (BBY), deboned breast yield (DBY) and thigh + drumstick yield (TDY). At 14 days of age, the use of creatine increased the live weight in the treatments B and F. Live weight was estatistic bigger in treatments (K and L) with inclusion of all animal by-product meals, added or not with creatine, on broilers diet. At 42 days of age, 5% of BM inclusion (TMT E) compromised the weight, real feed conversion and the use of creatine seemed to affect the weight in the treatment H. Live weight and feed conversion were compromised in the treatments K and L. In the others treatments, the performance of broilers fed with diets included with animal by-product meals, added or not with creatine, had the same performance those fed exclusively with vegetal products. It is concluded that inclusion of MBM, VM and FM can be used individually without to compromise the performance and carcass yield of broilers. Creatine addition didn t influence the final performance on test diets.

Parvoviruses is a viral disease characterized by an acute hemorrhagic gastroenteritis, caused by a canine parvovirus (CPV) that is stable in the environment, able to bear pH variations and high temperatures. It is resistant to many common disinfectants and can survive for many months in contaminated areas. There are two common clinical forms of the disease: the myocardial and the gastroenteric. This work had as objective to analyse microscopically the cardiopathy cases, diagnosticated macroscopically during the necropsy of dogs with parvovirus detected in faeces. In the 100 samples send to the Histopathology Laboratory, from the University of Uberaba, they get in the left ventricular myocardium the following alterations: myocarditis 38%, hemorrhage 43%, hyaline degeneration 21% and hyperemia 79%. Having been carried out the Qui-Quadrado test with a significance level of 0,05, we can conclude that there is association (p = 0,02) between the infected animals with the parvoviruses virus and the histopathologyc alterations observed in the left ventricular myocardium.

CHAPTER II: The liver of P. expansa was characterized morphohistologically. To this end, twenty livers from clinically healthy male and female Podocnemis expansa (giant Amazon river turtle), weighing from 2.0 to 4,5 Kg, supplied by the commercial breeder Fazenda Moenda da Serra, in Araguapaz, state of Goiás, Brazil, were analyzed macro- and microscopically. The coelomatic cavity was opened and the topography of the fresh organs was examined visually. After the histological preparation, the slides were stained with Hematoxylin and Eosin (HE), Periodic Acid-Schiff (PAS), Gomori Trichrome, Reticulin and Picrosirius. The liver of P. expansa is a voluminous organ with an approximately rectangular shape and brown coloration, varying from light to dark shades, and is divided into a right lobe, left lobe, and a central portion. The right lobe is the largest of the three portions. The gall bladder is located in a depression in the caudal portion of the right lobe, where the gall duct begins and empties into the duodenum. Histologically, the hepatocytes are arranged in the form of double cords surrounded by winding sinusoidal capillaries. In cross section, they resemble acini containing approximately two to five hepatocytes surrounding a probable central biliary canaliculus. The hepatocytes are polyhedral or pyramidal in shape, of uniform size, with a few central nuclei and others displaced peripherally, and the cytoplasm is little eosinophilic when analyzed by the HE staining technique. The parenchyma is supported by delicate reticular fibers surrounding hepatocytes and sinusoids. The parenchyma and perisinusoidal spaces contain large quantities of melanomacrophages, mainly close to the portal spaces. CHAPTER III: The objective was the morphological characterization of the liver of Phrynops geoffroanus, popularly known as the freshwater turtle (cágado-de-barbicha in Portuguese), using six specimens from the Uberabinha River in Uberlândia, state of Minas Gerais, Brazil. The analyses involved anatomical and histological methods, and the microscopic aspects were described after the histological preparation. Fragments of liver were fixed in 10% formalin and absolute alcohol, embedded in paraffin, sectioned into four μm thick slices, and stained with HE, PAS, Gomori Trichrome and Reticulin. The liver of P. geoffroanus is a large light brown organ speckled with black spots, which represent melanin deposits. It is divided into four lobes, called the right lateral, right medial, left lateral and left medial lobes. The hepatocytes in longitudinal section are similar to double cords surrounded by winding sinusoidal capillaries, polyhedral shaped, of varying sizes, with a vacuolized aspect and with peripherally displaced nuclei. The cytoplasm is little eosinophilic and highly reactive to PAS, suggesting abundant intracytoplasmatic glycogen. The hepatic parenchyma is supported by delicate reticular fibers surrounding hepatocytes and sinusoids. Large quantities of melanomacrophages are present in the parenchyma and perisinusoidal spaces, mainly close to the portal spaces.

In Brazil, information on ticks focus on the parasite species of domestic animals while those of wild animals, especially birds, are scarce and fragmented. Based on what was exposed, this study aimed to conduct a survey on the fauna of ticks and assess their importance in wild birds in an area of the cerrado of the Triângulo Mineiro under intense anthropogenic effect, the Natural Reserve of Clube de Caça e Pesca Itororó, Uberlândia, MG, Brazil. Besides assessing the infestation of birds by ticks to establish possible correlations with the environmental infestation and from domestic animals living nearby. Ticks were collected on birds from a small cerrado fragment in the reserve, and also collected from the environment and from domestic animals from neighboring properties. On the whole 238 passarine birds were captured from April 2008 to February 2009. From these 22 were infested with overall 31 ticks. Solely Amblyomma nodosum larvae and nymphs were recovered from these birds. Infestation of ticks host-seeking on the ground level were low and included all stages of Amblyomma cajennense, an Amblyomma dubitatum adult and larvae clusters of Boophilus microplus. Dogs from neighboring properties were infested with Rhipicephalus sanguineus adults, Amblyomma cajennense adults and nymphs, B. microplus nymphs; Horses had adults and nymphs of A. nitens, nymphs of B. microplus, nymph of A. cajennense. Bovines were infested solely with B. microplus (adults and nymphs).

Data on caprine tuberculosis in Brazil and its importance for public health are scarce. Therefore, the purpose of this study was to investigate the occurrence and examine epidemiological aspects of tuberculosis in dairy goat herds in the Triângulo Mineiro and Alto Paranaíba mesoregion in the state of Minas Gerais, Brazil. Two hundred and thirty-three goats from seven dairy farms were tuberculinized using the comparative cervical tuberculin test, taking as reference the procedures and interpretation criteria of standard results for this species. Of the animals subjected to the test, 1.29% (3/233) reacted positively, 2.14% (5/233) showed inconclusive reactions, and 96.57% (225/233) showed no reaction. All the goats that tested positive and one showing an inconclusive response were necropsied. Samples of lymph nodes, liver, lung and kidney presenting alterations were collected for a histopathological examination (hematoxylin-eosin). Material removed from abscesses and lymph node fragments were cultured in Stonebrink and Petragnani culture media. An epidemiological inquiry was carried out to analyze risk factors on the farms. The necropsied animals did not exhibit macroscopic lesions suggestive of tuberculosis or any histopathological alterations, nor did the cultures show Mycobacterium sp. growth. The goats prevalence rate to tuberculin reaction was 1.29%, which may represent a zoonotic risk in this mesoregion since these are dairy herds. The interpretation of the epidemiological records, allied to the results of the tuberculinization procedure, indicated positive reagents only on a farm where fresh cow milk was fed to newborn goat kids.

Leishmaniasis are zoonosis with different clinical manifestations according to the Leishmania species involved and the host immune response. Leishmania braziliensis complex is responsible for the majority of human Tegumentary Leishmaniasis cases in Brazil and it is endemic in the Triângulo Mineiro region. Our aim was to compare the pathogeny of two different isolates of Leishmania braziliensis complex, obtained from cutaneous lesions of patients from Triângulo Mineiro region, with Leishmania (Viannia) braziliensis reference strain (MHOM/BR/75/M2903). Comparision was done by histopathological and ultrastructural analisys of organs of experimentally infected Hamster golden (Mesocricetus auratus). For that, 106 stationary promastigotes of the two isolates and the reference strain were subcutaneously innoculated in the hind forepaw of Hamster that were killed after 15, 30, 60 e 120 days post infection and then were collected fragments of the skin, popliteal lymph node, liver, spleen and bone marrow. The organs were processed for histopathological and ultrastructural analysis. The results showed differents pathogeny between both isolates and the reference strain, characterized for differences in prepatent period of the macroscopy lesions, in the outcome of the histophatological lesions, in the parasitism and in the capacity to produce metastatic lesions in the lymph node, liver and spleen.

The aim of this study was to verify ovine health condition from Uberlândia county (MG), searching the prevalence of the main infections diseases that can reach ovine: leptospirosis, brucelosis (Brucella abortus and Brucella ovis), maedi-visna, Chamydophila spp., neosporosis and toxoplasmosis, associating these infections with risk factors. A total of 334 blood samples were collected randomly from both sex, different ages and breeds from 12 farms in Uberlândia county. A epidemiologic questionary was applied for each farm. The blood samples were submitted to Microscopic Aglutination Test (leptospirosis) Buffered Brucella Antigen Test (brucelosis Brucella abortus), Complement Fixation (Brucella ovis and Chlamydophila spp.), Agar Gel Immunodifusion (maedi-visna) and Indirect Fluorescent Antibody (neosporosis and toxoplasmosis) tests to search serology of these infections. The prevalences were: 22.2% to leptospirosis and titers from 1/100 to 1/3200, 3.3% to Chlamydophila spp. and titers from 1/32 to 1/64, 8.1% to neosporosis and titers from 1/50 to 1/3200 and 30.8% to toxoplasmosis. There were not reactive ovine to brucelosis (Brucella abortus and Brucella ovis) and maedi-visna. There was statistical significant difference (p<0.05) to reactive ovine to leptospirosis: male, breed, beef sheep and with presence of rodents. There was not statistical significant difference to brucelosis, maedi-visna, Chlamydophila spp. and neosporosis with risk factors associated (p>0.05).