Repositório RCAAP

Abstract In this work, it was determined the influence of temperature, pH and storage time on the enzymatic activity and stability of an extracellular fructosyltransferase (FTase E.C.2.4.1.9) from Aspergillus oryzae IPT-301 produced by submerged fermentation. The thermodynamic parameters showed a tendency for increasing enzyme denaturation with the rise in temperature. The maximum transfructosylation activity was obtained at the incubation pH 5.5. During storage at 4 °C, the transfructosylation activity decreased, whereas the hydrolytic activity increased, especially in the first nine hours, a time in which the enzyme presented 45.6% of its initial transfructosylation activity. These results contributed to the improvement of the conditions of storage, immobilization and use of the soluble fructosyltransferases (FTase) in fructooligosaccharide (FOS) production.

Abstract The stability of jabuticaba flakes produced by drum drying using cassava starch as additive was evaluated. Sorption isotherms at 25 ºC were determined. Samples were stored under controlled conditions (relative humidity = 60%, temperature = 20, 25 and 35 ºC) for until 235 days. The anthocyanin content and color parameters (L*, a* and b*) were monitored. GAB model led to the best adjustment of sorption isotherm, with a monolayer moisture value of 0.1596 g water/g dry basis. The visual observations did not point out agglomerations and darkening at aw values higher than 0.33 at 25 ºC, which can be considered the critical point. The degradation of anthocyanin and color parameters followed first and zero-order kinetic model, respectively. The half-life times (630 to 1450 days) showed good stability at 25 ºC, while the temperature acceleration coefficient (2.2 ≤ Q10 ≤ 3.2) and activation energy (15 ≤ Ea ≤ 19 kcal/mol) values demonstrated a high-temperature sensitivity.

Abstract The consumption and cultivation of curly kale in Brazil is recent and information on production systems and their acceptance by the consumer are scarce. The aim of this work was to evaluate the sensory characteristics of curly kales cultivated using the conventional and hydroponic systems, considering the treatments: cultivation under the conventional system, without fertilization (A), conventional system with organic fertilizers (C, E) and hydroponic system (F). The harvests were performed at 60, 90 and 120 days after planting for the plants cultivated using the conventional system and at 30 days for the hydroponic system. The total chlorophyll index content and the sensory analyses of ranking of difference and acceptance were analyzed. The lowest chlorophyll index in the curly kales was verified for the one cultivated under the hydroponic system. In the ranking test, the darkest green color was obtained for the curly kale produced using the conventional system and for the attributes aroma, sweet and bitter taste and crunchiness, there was no significant difference.

Abstract This study aimed to evaluate the survivability of Lactiplantibacillus plantarum 299v encapsulated in chitosan-coated calcium alginate beads with inulin as prebiotic in simulated gastrointestinal conditions and roselle juice. The concentration of calcium chloride and inulin for L. plantarum 299v microencapsulation was optimised and the survivability of free and microencapsulated L. plantarum was assessed under simulated gastrointestinal conditions. Storage stability of the optimised encapsulated L. plantarum 299v-inulin was determined throughout four (4) weeks of storage in roselle juice at 4 °C and 25 °C. The optimized formula for L. plantarum 299v was 2.0% (w/v) of calcium chloride and 3.0% (w/v) of inulin. Optimized calcium alginate-chitosan L. plantarum 299v microbeads with inulin did not affect (p > 0.05) the bead diameter, with a mean diameter of 685.27 μm, and microencapsulation efficiency of 95%. Encapsulated L. plantarum 299v with inulin showed higher survivability (>107 CFU/mL) than free cells and encapsulated L. plantarum 299v without inulin under simulated gastrointestinal conditions and after four (4) weeks of storage in roselle juice at 4 °C. The results indicate that co-extrusion encapsulation and addition of inulin had improved the viability of L. plantarum 299v in roselle juice by protecting probiotic against unfavourable gastrointestinal conditions and prolonged storage.

Resumo O leitelho, um subproduto da produção de manteiga, é altamente poluente devido à alta demanda bioquímica de oxigênio. Uma das características principais desse resíduo é a presença de fosfolipídeos, que o torna um ótimo emulsificante para ser aplicado em alimentos. Deste modo, o objetivo do presente trabalho foi estabelecer uma formulação para a fabricação de sorvete e verificar na mesma a influência do leitelho na substituição de leite desnatado. Para isso, foram elaboradas três formulações: tradicional (100% leite desnatado); com 50% de substituição de leite desnatado por leitelho, e outra com substituição total do leite desnatado por leitelho (100% leitelho). Esses sorvetes foram submetidos à análise de pH, atividade de água, incorporação de ar (overrun), derretimento e análise sensorial (aceitação, intenção de compra e comment analysis). A amostra de sorvete que teve 100% do leite desnatado substituído por leitelho foi a que apresentou melhores resultados de overrun, derretimento e sensoriais, sendo o sorvete mais aceito e com melhor intenção de compra. Portanto, concluiu-se que o leitelho pode ser indicado como sendo um substituto do leite desnatado na produção de sorvetes, permitindo assim um ótimo aproveitamento desse resíduo.

Resumo Cruá vermelho é um fruto aromático ainda pouco conhecido, originário da América Tropical, provavelmente do Brasil. É utilizado para fins alimentar, medicinal, repelente de insetos e aromatização de ambientes. Pouco se sabe acerca de suas características nutricionais. Diante do exposto, o presente trabalho visou realizar a caracterização física, físico-química e nutricional do fruto cruá vermelho. Os frutos foram caracterizados quanto a composição centesimal, pH, sólidos solúveis (SS), acidez titulável (AT), cálculo da ingestão diária recomendada (IDR), antocianinas, carotenoides totais, vitamina C e atividade antioxidante da polpa. A estabilidade dos carotenoides, da vitamina C e da cor dos frutos foi acompanhada durante 21 dias de armazenamento sob congelamento a -18 °C. Os carotenoides, a vitamina C e a cor do fruto foram afetados pelo congelamento durante o armazenamento. O fruto apresenta características apreciáveis do ponto de vista nutricional e funcional, uma vez que o consumo diário de 100 g de sua polpa fornece uma apreciável fração de nutrientes recomendados para ingestão diária por um indivíduo adulto. O fruto apresenta um baixo valor calórico, fato interessante para preparações dietéticas, além de apresentar capacidade antioxidante e compostos fitoquímicos de interesse para a saúde humana, como carotenoides e antocianinas. Em relação à preservação dos compostos bioativos apresentados pelo fruto, verificou-se que o congelamento não foi eficaz para a preservação de carotenoides e vitamina C. Diante de todas as características apresentadas, cruá vermelho se apresenta como uma excelente alternativa para inclusão na alimentação humana.

Abstract This study aimed to investigate the extraction of total phenolic compounds of sugarcane bagasse using various solvents. In addition, the Sugarcane Bagasse Water Extract (SBWE) was used in refrigerated fresh meat as natural preservative. The fresh meat was dipped into water solutions containing various phenolic compounds concentrations (T1:125, T2:250 and T3:500 ppm). During 10 days of storage at 4 °C for all the treated samples were compared with untreated one. The results revealed that SBWE showed relevant values of total phenolic compounds (17.90 mg/g) and total flavonoids content (4.50 mg/g), as well as 45.90% of antioxidant content. On the other hand, microbiological examination and sensory evaluation have turned out to be the best treatment for T3:500 ppm. The SBWE showed an antibacterial impact on Staphylococcus sp. and a reduction in the Total Plate Count and in the group of Psychrotrophs. The shelf-life of refrigerated fresh meat treated with SBWE by dipping it into water solutions was also extended to more than 10 days.

Abstract Among the main challenges regarding the food industry, it could be noted that it is necessary to make food products with the required quality and affordable cost. Computing modeling methods solved such tasks by optimizing food recipes based on specific product criteria. However, the problem of optimizing school meals still needs to be addressed. Cottage cheese and culinary products are valuable sources of protein and calcium. They are particularly in demand for schoolchildren’s nutrition. This research aimed to develop molded culinary products with cottage cheese (cheese pancakes) based on the criteria convolution method using the Harrington-Mecher desirability function, i.e., a generalized desirability function in order to meet the physiological needs of school-aged children. The components used were cottage cheese, two additives from Jerusalem artichoke: (M1: «Jerusalem artichoke, pumpkin, pear» or M2: Jerusalem artichoke, quince, mountain ash), egg, and dry components (barley flakes or oat flour). Components were combined at specific ratios; the masses obtained were subject to structural-mechanical and sensory analysis. To determine the optimal equation of the dependence of the critical shear stress (yield stress) regarding the quantitative recipe composition, regression analysis was conducted, followed by Multi-criteria optimization based on Harrington’s desirability function. Two cheese pancake recipes were proposed, as they showed optimum quality characteristics of the final product: Recipe 1 (%): Cottage cheese 74.0, plant additives 14.0, oat flour 7.7, eggs 4.3; Recipe 2 (%): Cottage cheese 69.0, plant additives 17.0, flakes barley 10.0, eggs 4.0. The proposed approach was effective in optimizing various molded culinary products and represents a methodological basis for finding new innovative technological solutions in the food industry and public catering.

Resumo O objetivo deste estudo foi a elaboração e a caracterização reológica de um iogurte sabor bacuri com reduzido teor de gordura. Foram elaborados três iogurtes a partir dos tipos de leite UHT integral, semidesnatado e desnatado. As análises reológicas foram realizadas em quatro diferentes temperaturas (5 °C, 15 °C, 30 °C e 45 °C) e os dados experimentais foram ajustados ao modelo de Ostwald-de-Waelle. A relação entre temperatura e viscosidade aparente foi descrita por uma equação tipo Arrhenius. Os valores de tensão de cisalhamento e viscosidade aparente dos iogurtes diminuíram com o aumento da temperatura, bem como com a redução do teor de gordura. O modelo utilizado mostrou-se adequado para descrever o comportamento reológico dos iogurtes, segundo os parâmetros estatísticos avaliados. Os baixos valores obtidos para o índice de comportamento indicaram um comportamento característico de fluidos pseudoplásticos (n<1). A equação tipo Arrhenius descreveu de modo satisfatório o efeito da temperatura sobre a viscosidade aparente. Os valores da energia de ativação (Eat) foram de 2,66 kcal g mol-1, 3,81 kcal g mol-1 e 3,93 kcal g mol-1, respectivamente, para as amostras de iogurte integral, semidesnatado e desnatado. Na caracterização físico-química, os teores de gordura se mostraram próximos para os iogurtes desnatado e semidesnatado, não havendo diferença significativa. O iogurte semidesnatado e o desnatado podem ser considerados com reduzido teor de gordura, pois sofreram uma diminuição no teor de lipídios de 75% e 79,1%, respectivamente.

Abstract Important functional compounds present in fruits are often lost in technological processes and during storage. Microencapsulation technique allows maintaining the compounds of interest and adding value to the product using functional encapsulating materials. This work aimed to produce microencapsulated guava pulp using the spray-drying technique and a functional encapsulant material, i.e., a mix of inulin and maltodextrin. The guava pulp was analyzed for centesimal composition, carotenoid content, and antioxidant activity. The microspheres were analyzed for retention of carotenoids, antioxidant activity over time, and morphology by Scanning Electron Microscopy (SEM) and X-ray diffraction. Two proportions of coating material could maintain the antioxidant activity of guava pulp. The microencapsulation with a higher percentage of inulin is a preferred option due to the good results of retention and stability regarding antioxidant activity over time, relevant retention of the carotenoid content, and a more stable microstructure. In addition, inulin can add value to powders owing to its inherent functional properties. The product obtained in the study is innovative and interesting, as well as may provide a capable use of these materials as encapsulated agents. In fact, it can be considered a potential functional ingredient.

Abstract Sweet cassava can be sold frozen, i.e., frozen food, to facilitate preparation and consumption. This study aimed to evaluate physical-chemical characteristics, cooking time, microbiological quality and sensory attributes regarding the varieties BRS Aipim Brasil and Eucalipto in frozen storage. The roots were washed in water, sanitized, cut into cylinders, peeled, sanitized, drained, packed in nylon/ low density polyethylene packages and stored at -18 °C, being evaluated every 30 days, approximately. The Eucalipto variety showed the lowest moisture (61.98%) and the highest pulp yield (71.41%) and starch content (33.45%). However, the BRS Aipim Brasil variety had lower color intensity (11.48) and greater color angle (97.45°) than Eucalipto. The two cassava genotypes showed no difference in terms of acidity, luminosity, soluble solids, total sugar content and pH. The Eucalipto variety showed the shortest cooking time (25 minutes) and moisture (62.92%) concerning the five storage times that were evaluated. This variety also had the highest starch content during storage, except at 31 days. The Eucalipto variety was more accepted for the color, aroma and overall impression attributes. Regarding flavor and texture, the two varieties were considered similar and were classified between the hedonic terms “like slightly” and “like moderately”. The sensory acceptance of the roots was not altered during storage and the grades attributed were above 6.0, which represents the minimum acceptance limit, for all evaluated attributes. Cassava frozen at -18 °C can be marketed for 120 days without microbiological risk, and without significant changes in its physical-chemical and sensory characteristics.

Resumo O objetivo deste trabalho foi desenvolver formulações de misturas para preparo de bolos de caneca, sem ovos e sem leite, com farinhas de cascas e sementes de uvas, além de avaliar características físico-químicas, tecnológicas e sensoriais, com enfoque nos efeitos dos ácidos ascórbico e cítrico sobre os bolos, com substituição parcial da farinha de trigo por farinha de bagaço de uva. Aos produtos, foram incorporados 5% de farinhas de cascas e sementes em relação à farinha de trigo. Foram realizadas análises de polifenóis, atividade antioxidante, cor, pH, volume específico, perda de massa e sensorial. Entre as formulações com farinha de uva, a amostra com adição de ácido ascórbico apresentou maior (p < 0,05) atividade antioxidante, medida pelo método ABTS, do que as amostras sem adição de ácidos. Entretanto, a formulação não apresentou diferença significativa (p > 0,05) quando comparada com a formulação com a adição de ácido cítrico ou a combinação de ácidos. De acordo com o coeficiente de correlação de Pearson, quanto mais ácido (menor pH) se mostrou o bolo, maior foi a tendência de aumento na concentração de polifenóis totais, antocianinas e compostos com atividade antioxidante. Com relação ao volume específico, a maioria das formulações controles apresentou os maiores volumes em relação às suas respectivas amostras com farinha de uva. Assim, a incorporação dos ácidos ascórbico e cítrico nos bolos com farinha de bagaço de uva possivelmente acarretou estabilização da coloração vermelha e o bolo com farinha de bagaço de uva e ácido ascórbico apresentou as maiores quantidades de compostos fenólicos totais, quando comparado aos outros bolos com o subproduto, além de ter sido um dos bolos mais bem aceitos pelos provadores, demonstrando o potencial de mercado para esses produtos alimentícios inovadores.

Abstract Brazil nuts are often used for direct consumption or in the preparation process of water-soluble extract. After obtaining the water-soluble extract, a large amount of Brazil nut residues with good sensory characteristics are generated. Thus, this study aimed to dry Brazil nut processing residues in layers with different thicknesses in a direct solar dryer as well as by direct exposure to the sun, in order to fit different mathematical models to the experimental data of drying kinetics, and calculate the drying rates and effective diffusivity. The drying procedures began at 9 a.m. on a concrete base, for samples dried by direct exposure to the sun, and in a solar dryer constructed with expanded polystyrene foam zinc plated and a glass cover. The mass loss of the samples was monitored by weighing at regular times until the hygroscopic equilibrium was obtained. The direct solar dryer had temperatures about 80% higher than those recorded in the open environmental air temperature. Drying rates were higher in dehydrations performed in the solar dryer compared to the drying by exposure to the sun. The Midilli model was selected as the most adequate for predicting the drying of the samples under all experimental conditions, showing coefficients of determination above 0.99. The effective diffusion coefficients of moisture were higher in samples dehydrated in the solar dryer when compared to those dried by exposure to the sun. Regarding the research conducted under the experimental conditions of this study, the performance of the solar dryer to dry Brazil nut processing residues was satisfactory.

Abstract One of the actual areas of processing the aquatic biological resources is the production of chilled fish semi-finished products, the most prepared for heat treatment. Such products have a very limited shelf life, because fish raw materials have high enzymatic activity. This research work aimed to improve the technology of chilled fish semi-finished products from Japanese mackerel with an extended shelf life. To achieve this goal, Japanese mackerel was injected with multicomponent salting media, containing food additives that slow down microbial contamination, have a bacteriostatic effect and assist in improving the organoleptic characteristics of the finished product. New receptions of salting multicomponent compositions provide faster salting and ripening of raw materials compared to the control sample. Salt concentration in fish, amine nitrogen and peroxide value were determined by titration methods. The total number of mesophilic aerobic and facultative anaerobic microorganisms (КMAFAnM) in fish semi-finished products was determined by identifying the number of grown colonies of microorganisms on nutrient media from agar. The treatment of Japanese mackerel with developed salting media, including moisture-retaining components, organic acids and flavoring additives with anti-bactericidal properties, improves the organoleptic characteristics of the finished product, promotes more salting and maturation of fish, as well as reducing the number of microorganisms (KMAFAnM). The increase in amine nitrogen content ranged from 1.4 g/kg (at the beginning of the salting) to 1.55 g/kg in the control and up to 3.05 g/kg in the test samples by the end of the salting. Salt concentration in Japanese mackerel treated with control salting media amounted to 1.19%, new developed salting media - from 2.84% to 3.17%. The total abundance of microorganisms in Japanese mackerel was 2.0 x 102 CFU/g, after salting it decreased to 0.1 to 3.0 x 101 CFU/g depending on the formulation of media for salting. According to the research results, the rational duration of the salting of Japanese mackerel, with new media for salting was 4 to 5 h at a temperature of 6 °С to 8 °С. Along with the salting media developed by us, the use of modern packaging materials in the technology provided chilled culinary semi – finished products from Japanese mackerel with longer shelf life of up to 20 days at a temperature of 0 °С to 5 °C, and humidity 95% to 98%.

Abstract Ora-pro-nobis (OPN) (Pereskia aculeata Miller) is a non-conventional edible plant rich in protein, fibres and minerals. The innovation of this work is based on the sustainability production of the aqueous extract (green solvent) containing iron and bioactive compounds as well as employing a native plant from Brazil. A screening of phytochemicals components, phenolic compounds, flavonoids and tannins contents were performed using OPN extract. The antioxidant activity of the OPN aqueous extract was determined by three different assays as following: 2,2-diphenyl-1-picrylhydrazyl (DPPH•); Ferric Reducing Antioxidant Power (FRAP); and Oxygen Radical Absorbance Capacity (ORAC). The OPN dried leaves presented high protein and minerals contents. Indeed, the Fourier-Transform Infrared Spectroscopy (FTIR Spectroscopy) analysis performed in OPN aqueous extract confirmed the appearance of representative functional groups of the bioactive compounds. Overall the results suggested that it is possible to use simple aqueous solvent to produce OPN extract rich in iron, bioactive compounds and within antioxidant activity that could be potentially used as functional food ingredient.

Abstract The objective of this study was to evaluate the response of biological activity measured by the Biospeckle Laser technique applied to mangoes (Mangifera indica L.) subjected to mechanical damage at different stages of maturation. The tests were carried out twice a week for twenty-one days, and 40 mangoes of the variety Tommy Atkins were used. The procedure was divided into two stages: the first simulated the impact on fruits, and in the second stage, the laser light was applied to the impacted area. After laser application, the images of all fruits were submitted to graphic processing, in order to obtain maps of biological activity of the fruits. The Biospeckle laser technique associated with image analysis allowed the identification of distinct biological activity levels in the mangoes, certifying its potential application as a non-destructive test tool, in the monitoring of biological activity in fruit samples.

Abstract The yeast autolysis process - an endogenous and irreversible lytic event, which occurs in cells caused by the action of intracellular enzymes, proteases and carbohydrases - is a well-known and an economic process, however, there is a constant risk of serious microbial contamination since there are many nutrients in the broth and this process is slow, favoring the growing of pathogens. The present work comes up with an attempt to accelerate the autolysis of Saccharomyces cerevisiae with focus on the high yield of yeast extract production through a fast, economic and simple technology. The proposed strategy is based on decreasing the pH of the yeast suspension at the beginning of autolysis through an acid shock to activate the cell autolytic system under stressful conditions of temperature and pH. The influence of cell concentration, temperature, time and acid shock at the beginning of the autolysis on yeast extract yields were studied. The best yields of proteins and total solids were observed for autolysis treated with acid shock (H2SO4 10 µL/g of dried yeast and final pH 4.4) at 60 °C (36, 84% of protein and 48, 47% of total solids extracted) and gradual increase of temperature 45 to 60 °C (41.20% of protein and 58.48% of total solids extracted). The shock could increase the speed of the process since the control reached about 30% of extract at 60 °C and the same experiment, however, with acid shock reached more than 43% in 12 h. When considering time in an industrial scale, it could be noted that the time was very important for the productivity as well as avoiding risk of pathogen contamination in autolysis. These results were very relevant for industrial purposes in the production of yeast extract, autolyzed yeast and glucan and mannan.

Abstract The sustainable food valorization is capable to stimulate the local products consumption with quality and nutritional security. In this scenario, Unconventional Food Plants (UFPs) deserve attention representing an alternative for rural communities and contributing to the local and regional economy. This work aimed to add nutritional information, once it evaluates total Fe concentration, Fe-species (water soluble, acid soluble, and inorganic), proteins, and Fe- proteins distribution in beldroega (Portulaca oleracea L.), guasca (Galinsoga parviflora Cav.), ora-pro-nóbis (Pereskia aculeata Mill.), piracá (Vernonia scorpioides (Lam.) Pers.), and trapoeraba (Commelina benghalensis L.). Considering the Fe total concentration, the studied UPFs can be excellent Fe sources, when compared to foods of plant origin widely consumed and cultivated, detaching the guasca leaves (687±19 µg g-1). However, the guasca leaves showed low concentration of Fe associated to macromolecules and Fe soluble species (1.6±0.3 µg g-1). These results may justify the low bioavailability of Fe species. On the other hand, beldroega leaves presented the highest concentration of inorganic Fe (1.3±0.2 µg g-1). In the UFPs, there is high glutelins concentration when compared to the other protein groups, meaning that UFPs can be low-cost alternative to supplementing protein intake. Finally, for majority UFPs, except guasca leaves, Fe is mainly associated to albumins, being a good source of bioavailable Fe species.

Abstract The comparative study regarding complexes coacervated between Bovine Serum Albumin (BSA) and different polysaccharides, Pectin (PEC) and Gum Acacia (GA), was carried out by evaluating the influence of different ratios (protein:polysaccharide) and sodium chloride (NaCl) concentrations on turbidity and zeta potential. The BSA:PEC complexes were formed in a 10:1 ratio whereas BSA:GA at 3:1. The complexation pH showed different behavior, BSA: PEC complexes exhibited maximum turbidity in a wide pH range (4.9 to 1.5), while BSA: GA had maximum turbidity at pH 3.5. The increase in the concentration of NaCl negatively influenced the complexation. The NaCl concentration of 0.40 mol L-1 suppressed the interaction in BSA:PEC (10:1) and reduced the range formation of BSA:GA (3:1). The Fourier Transform Infrared (FTIR) demonstrated the participation not only of electrostatic interactions, but also of hydrogen bonds in the complexation. This initial study elucidated fundamental aspects about the formation of coacervate complexes between BSA:GA/PEC that assist in directing its application in food products especially, in acidic matrices (pH~4.0) as well as with low concentration of salts, in view of the effect of pH on maximum formation and sensitivity to NaCl. These complexes can be added directly to products in order to add nutritional value or even be used as a new matrix for the encapsulation of bioactive compounds.

Abstract Different types of microorganisms are important in cheese-making because of the contributions their metabolism offers during the process. Few microorganisms present in Colonial cheese are known, in addition to the ones that are introduced to kick-start the processes or the ones that are associated with infections or poisonings. This study aimed to identify, by MALDI-TOF and/or DNA sequencing, the bacteria and yeasts isolated from samples collected in the main stages of Colonial cheese production, i.e., a type of cheese produced in the southern region of Brazil. The lytic capacity of these microorganisms at 5 °C and 30 °C was also evaluated. The 58 bacterial strains were distributed in 10 species among the genera Bacillus, Citrobacter, Klebsiella, Lactococcus, Paenibacillus, Staphylococcus and Raoutella. From the 13 yeasts strains analyzed, three species were identified as following: Candida pararugosa; Meyerozyma guilliermondii; and Rhodotorula mucilaginosa. In three yeasts isolates it was possible to identify only the genus Candida sp. and Trichosporon sp. The species L. lactis (48%) and M. guilliermondii (46%) were, respectively, the predominant bacteria and yeasts species isolated. The highest microbial lytic activity observed was at 30 °C. Lipase activity on isolates was proportionally more observed with yeasts and proteolytic activity with bacteria. Lower caseinase and lipase activity was observed at 5 °C, demonstrating the importance of refrigeration in controlling microbial activity. This research highlighted the cultivation of some microorganisms that are part of the Colonial cheese microbiota as well as that several of them can hydrolyze various compounds present in milk and that could be associated with its maturation or, in uncontrolled circumstances, could be the cause of product deterioration.