Repositório RCAAP

O desenvolvimento tecnológico é indissociável da sua implicação ética e política. Identificados importantes dilemas da participação na cidade contemporânea – a captura da partilha de decisões em processos autoritários, a abertura tanto verdadeira como ilusória que a cultura digital propicia para a autonomia de indivíduos e coletivos –, considera-se que a lente da tecnopolítica pode ser útil para compreender seu percurso histórico, contextualizar sua condição contemporânea e permitir uma apreciação mais informada das escolhas para o futuro. Nesse sentido, procurou-se construir uma narrativa sobre as tecnopolíticas da participação através de um estudo genealógico, identificando orientações teóricas, experiências práticas, marcos legais e históricos, a partir de literatura de referência, compilações de práticas e sítios eletrônicos de projetos e de autores. O resultado é uma linha do tempo, disponibilizada de forma online e interativa, na qual identificou-se cinco marcos, tendo em conta as principais inovações tecnopolíticas observadas: participação para a cidadania, participação para a autonomia, participação comunitária, participação institucionalizada e participação em rede. O estudo reforça o papel crucial da informação no desenvolvimento das tecnopolíticas da participação e ressalta oportunidades e desafios que a participação em rede oferece para a sua democratização.

Currently, the main public health concern worldwide is the COVID-19 pandemic, caused by SARS-CoV-2, which was recently discovered and described. Due to its high pathogenicity and infectiousness, it is necessary to determine the risk healthcare professionals face every day while dealing with infected patients and contaminated biological samples. The purpose of this study was to assess Portuguese Biomedical Scientists' risk of COVID-19 exposure and related stress appraisals. One hundred and forty participants completed online versions of the WHO's Risk Assessment and Management of Exposure Survey and the Stress Appraisal Measure. Participants worked mainly in outpatient settings (45%), and in emergency services (28%). Twenty three percent of participants were exposed to COVID-19 through community exposure, and 39% through occupational exposure. Although 95% reported using personal protective equipment (PPE), 83.6% were at high risk of infection. However, the use of some types of PPE was related to the risk of exposure. Participants reported moderate perceptions of stress and threat, but also moderate perceptions of control over the situation. These results may contribute to a more efficient risk management of these professionals and prevent disease transmission in hospitals and communities.

Background Atrial fibrillation (AF) is a major risk factor for mortality. The prevalence, clinical correlates, and prognostic impact of AF in Takotsubo syndrome (TTS) have not yet been investigated in a large patient cohort. This study aimed to investigate the prevalence, clinical correlates, and prognostic impact of AF in patients with TTS. Methods and Results Patients with TTS were enrolled from the International Takotsubo Registry, which is a multinational network with 26 participating centers in Europe and the United States. Patients were dichotomized according to the presence or absence of AF at the time of admission. Of 1584 patients with TTS, 112 (7.1%) had AF. The mean age was higher (P<0.001), and there were fewer women (P=0.046) in the AF than in the non-AF group. Left ventricular ejection fraction was significantly lower (P=0.001), and cardiogenic shock was more often observed (P<0.001) in the AF group. Both in-hospital (P<0.001) and long-term mortality (P<0.001) were higher in the AF group. Multivariable Cox regression analysis revealed that AF was independently associated with higher long-term mortality (hazard ratio, 2.31; 95% CI, 1.50-3.55; P<0.001). Among patients with AF on admission, 42% had no known history of AF before the acute TTS event, and such patients had comparable in-hospital and long-term outcomes compared with those with a history of AF. Conclusions In patients presenting with TTS, AF on admission is significantly associated with increased in-hospital and long-term mortality rates. Whether antiarrhythmics and/or cardioversion are beneficial in TTS with AF should thus be tested in a future trial.

Background and purpose: Short-interval intracortical inhibition by threshold tracking (T-SICI) has been proposed as a diagnostic tool for amyotrophic lateral sclerosis (ALS) but has not been compared directly with conventional amplitude measurements (A-SICI). This study compared A-SICI and T-SICI for sensitivity and clinical usefulness as biomarkers for ALS. Methods: In all, 104 consecutive patients referred with suspicion of ALS were prospectively included and were subsequently divided into 62 patients with motor neuron disease (MND) and 42 patient controls (ALS mimics) by clinical follow-up. T-SICI and A-SICI recorded in the first dorsal interosseus muscle (index test) were compared with recordings from 53 age-matched healthy controls. The reference standard was the Awaji criteria. Clinical scorings, conventional nerve conduction studies and electromyography were also performed on the patients. Results: Motor neuron disease patients had significantly reduced T-SICI and A-SICI compared with the healthy and patient control groups, which were similar. Sensitivity and specificity for discriminating MND patients from patient controls were high (areas under the receiver operating characteristic curves 0.762 and 0.810 for T-SICI and A-SICI respectively at 1-3.5 ms). Paradoxically, T-SICI was most reduced in MND patients with the fewest upper motor neuron (UMN) signs (Spearman ρ = 0.565, p = 4.3 × 10-6 ). Conclusions: Amplitude-based measure of cortical inhibition and T-SICI are both sensitive measures for the detection of cortical involvement in MND patients and may help early diagnosis of ALS, with T-SICI most abnormal before UMN signs have developed. The gradation in T-SICI from pathological facilitation in patients with minimal UMN signs to inhibition in those with the most UMN signs may be due to progressive degeneration of the subset of UMNs experiencing facilitation.

We have experimented with isolated cardiomyocytes of mollusks Helix. During the whole-cell patch-clamp recordings of K+ currents a considerable decrease in amplitude was observed upon repeated voltage steps at 0.96 Hz. For these experiments, ventricular cells were depolarized to identical + 20 mV from a holding potential of - 50 mV. The observed spontaneous inhibition of outward currents persisted in the presence of 4-aminopyridine, tetraethylammonium chloride or E-4031, the selective class III antiarrhythmic agent that blocks HERG channels. Similar tendency was retained when components of currents sensitive to either 4-AP or TEA were mathematically subtracted. Waveforms of currents sensitive to 1 and 10 micromolar concentration of E-4031 were distinct comprising prevailingly those activated during up to 200 ms pulses. The outward current activated by a voltage ramp at 60 mV x s-1 rate revealed an inward rectification around + 20 mV. This feature closely resembles those of the mammalian cardiac delayed rectifier IKr.

Na perspectiva segundo a qual os indivíduos vão construindo a sua carreira à medida que tomam as suas decisões e procuram corresponder às expectativas sociais, os trabalhadores necessitam de investir e desenvolver as suas competências de modo a se adaptarem e sobreviverem num mercado de trabalho exigente e sem fronteiras. Actualmente, os trabalhadores são, assim, cada vez mais confrontados com desafios que lhes exigem uma crescente preocupação com o desenvolvimento das suas carreiras, uma maior flexibilidade e diversificação da força de trabalho, e a capacidade para se adaptarem às alterações do mercado de trabalho. É neste sentido que surge a importância de conceitos, tais como Adaptabilidade e Empregabilidade, cuja relação constitui o objectivo do presente estudo. Foram aplicados o Inventário sobre Adaptabilidade e o Inventário sobre Empregabilidade a uma amostra de 64 trabalhadores de uma organização do sector da construção civil. Os resultados obtidos tendem a responder às questões de investigação inicialmente formuladas, tendo-se verificado uma forte relação entre os dois conceitos, e correlações significativas entre as diferentes dimensões da Adaptabilidade e da Empregabilidade. Apresentam-se, ainda, a discussão dos resultados, as limitações do presente estudo e futuras linhas de investigação, bem como algumas implicações para as práticas de gestão de recursos humanos.

Functional brain connectivity (FBC) has previously been examined in autism spectrum disorder (ASD) between-resting-state networks (RSNs) using a highly sensitive and reproducible hypothesis-free approach. However, results have been inconsistent and sex differences have only recently been taken into consideration using this approach. We estimated main effects of diagnosis and sex and a diagnosis by sex interaction on between-RSNs FBC in 83 ASD (40 females/43 males) and 85 typically developing controls (TC; 43 females/42 males). We found increased connectivity between the default mode (DM) and (a) the executive control networks in ASD (vs. TC); (b) the cerebellum networks in males (vs. females); and (c) female-specific altered connectivity involving visual, language and basal ganglia (BG) networks in ASD-in suggestive compatibility with ASD cognitive and neuroscientific theories.

Future climate for the Mediterranean climatic region is expected to bring an increase in temperatures, decrease in the precipitation quantity and shifts in the seasonal precipitation pattern. Although the impacts of climate change on water resources have been relatively well explored for the Mediterranean climatic region, the specific consequences for reservoirs and, in particular, water availability and irrigation issues have been less studied. The objective of this work is two-fold: (i) to assess the impacts of future climate changes on water resources availability, quality (focusing on phosphorus loads as this is the limiting nutrient for eutrophication) and irrigation needs for two multipurpose reservoirs in southern Portugal; (ii) to suggest climate change adaptation strategies, especially for the agricultural sector. To this end, the SWAT model was first calibrated against existing data on reservoir inflows as well as phosphorus loads. Then, SWAT was run with climate derived EURO-CORDEX models (RCA4/RACMO22E) for four periods (1970–2000, 2010–2040, 2040–2070 and 2070–2100). Water availability was analysed using the Water Exploitation Index (WEI) that was calculated for both reservoirs combining changes of inflows and irrigation requirements. The results indicated that climate change will negatively impact water availability in both reservoirs, especially under RCP8.5. In the case of the Monte Novo reservoir, future domestic water supply could be constrained by water quality problems related with phosphorus loads. For Vigia reservoir, the high water exploitation will lead to water scarcity problems, mainly as this reservoir on present-day conditions is restrictive on irrigation requirements. Adaptation strategies such as the implementation of high end technology (e.g. soil moisture and plant water stress probes, satellite imagery and drones to evaluate water stress – NDVI) as well as the renewal of the irrigation network and adequate crop selection can help attenuating the effects of climate change on the water resources in this region.

Amphibians of high mountain lakes face many threats related to global change, including novel pathogens, development, climate change and overexploitation. However, the foremost threat is the presence of non-native fish. One of the objectives of the LIFE+ LIMNOPIRINEUS project (2014–2019) was the recovery of protected amphibian communities (including the endemic Calotriton asper) in eight naturally fishless Pyrenean high mountain lakes, by controlling or eradicating non-native trout or minnows. During the summer months of 2015 to 2019, we removed 95–100% of the fish present in these lakes, and monitored changes in their amphibian populations, as well as surveyed 56 nearby control lakes with or without fish. We found rapid natural recovery of amphibian communities as fish removal work progressed. The fish-removal lakes achieved typical richness figures for the area one year after fish removal began, and typical species abundances after three years (with the only exception of Rana temporaria). We documented a total of 16 colonisation events, all by amphibian species from the same valley. The two earliest colonisation events were observed in the year in which fish removal began, with eight events the following year. The lack of colonisation from nearby valleys in the study period highlights the crucial role of nearby residual populations not affected by human impacts. We show that whole amphibian communities from high mountain lakes recover rapidly after eliminating or reducing non-native fish, proving that this is a powerful tool to improve the conservation status of endangered amphibians.

Surface runoff generation capacity can be modified by land-use and climate changes. Annual runoff volumes have been evaluated in a small watershed of tropical forest (Brazil), using the Soil and Water Assessment Tool (SWAT) model. Firstly, the accuracy of SWAT in runoff predictions has been assessed by default input parameters and improved by automatic calibration, using 20-year observations. Then, the hydrological response under land uses (cropland, pasture and deforested soil) alternative to tropical forest and climate change scenarios has been simulated. SWAT application has showed that, if forest was replaced by crops or pasture, the watershed’s hydrological response would not significantly be affected. Conversely, a complete deforestation would slightly increase its runoff generation capacity. Under forecasted climate scenarios, the runoff generation capacity of the watershed will tend to decrease and will not be noticeably different among the representative concentration pathways. Pasture and bare soil will give the lowest and highest runoff coefficients, respectively.

The negative hydrological effects of wildfire are very difficult to predict in Mediterranean forest ecosystems, due the intrinsic climate and soil characteristics of these areas. Among the hydrological models simulating surface runoff and soil erosion in these environmental contexts, the semi-empirical Morgan-Morgan-Finney (MMF) model can ensure the representation of the main physical processes, while offering ease of use and limiting the number of input parameters. However, literature reports very few modelling studies using MMF in burned areas of the Mediterranean environment with or without post-fire rehabilitation measures. To fill this gap, the capacity of the MMF model to predict the seasonal surface runoff and soil loss in a Mediterranean forest was verified and improved for unburned plots and areas affected by a wildfire, with and without post-fire straw mulch treatment. The application of MMF with default input parameters (set up according to the original guidelines of the model’s developers) led to poor performance. Conversely, after introducing some changes in input data for both the hydrological and erosive components (seasonal values of evapotranspiration, reduction of the soil hydrological depth, including soil water repellency effects in burned soils, and modelling erosive precipitation only), MMF was able to predict seasonal runoff volumes and soil loss with good reliability in all the experimented conditions. This modelling experiment has shown the capacity of the MMF model to simulate the seasonal hydrological and erosion response of the experimental unburned and burned soils of Mediterranean semi-arid forests. Although more research is needed to validate the model's prediction capacity in these conditions, the use of MMF as a management tool may be suggested to predict the hydrogeological risk in these delicate ecosystems threatened by wildfire, as well as to evaluate the potential efficiency of soil treatments after fire.

Brassica is one of the most economically important genus of the Brassicaceae family, encompassing several key crops like Brassica napus (cabbage) and broccoli (Brassica oleraceae var. italica). This family is well known for their high content of characteristic secondary metabolites such as glucosinolates (GLS) compounds, recognize for their beneficial health properties and role in plants defense. In this work, we have looked through gene clusters involved in the biosynthesis of GLS, by combining genomic analysis with biochemical pathways and chemical diversity assessment. A total of 101 Brassicaceae genes involved in GLS biosynthesis were identified, using a multi-database approach. Through a UPGMA and PCA analysis on the 101 GLS genes recorded, revealed a separation between the genes mainly involved in GLS core structure synthesis and genes belonging to the CYP450s and MYBs gene families. After, a detailed phylogenetic analysis was conducted to better understand the disjunction of the aliphatic and indolic genes, by focusing on CYP79F1–F2 and CYP81F1–F4, respectively. Our results point to a recent diversification of the aliphatic CYP79F1 and F2 genes in Brassica crops, while for indolic genes an earliest diversification is observed for CYP81F1–F4 genes. Chemical diversity revealed that Brassica crops have distinct GLS chemo-profiles from other Brassicaceae genera; being highlighted the high contents of GLS found among the Diplotaxis species. Also, we have explored GLS-rich species as a new source of taxa with great agronomic potential, particularly in abiotic stress tolerance, namely Diplotaxis, the closest wild relatives of Brassica crops.

Ammonia and ammonium have received less attention than other forms of air pollution, with limited progress in controlling emissions at UK, European and global scales. By contrast, these compounds have been of significant past interest to science and society, the recollection of which can inform future strategies. Sal ammoniac (nūshādir, nao sha) is found to have been extremely valuable in long-distance trade (ca AD 600–1150) from Egypt and China, where 6–8 kg N could purchase a human life, while air pollution associated with nūshādir collection was attributed to this nitrogen form. Ammonia was one of the keys to alchemy—seen as an early experimental mesocosm to understand the world—and later became of interest as ‘alkaline air’ within the eighteenth century development of pneumatic chemistry. The same economic, chemical and environmental properties are found to make ammonia and ammonium of huge relevance today. Successful control of acidifying SO2 and NOx emissions leaves atmospheric NH3 in excess in many areas, contributing to particulate matter (PM2.5) formation, while leading to a new significance of alkaline air, with adverse impacts on natural ecosystems. Investigations of epiphytic lichens and bog ecosystems show how the alkalinity effect of NH3 may explain its having three to five times the adverse effect of ammonium and nitrate, respectively. It is concluded that future air pollution policy should no longer neglect ammonia. Progress is likely to be mobilized by emphasizing the lost economic value of global N emissions ($200 billion yr−1), as part of developing the circular economy for sustainable nitrogen management. This article is part of a discussion meeting issue ‘Air quality, past present and future’.

Entropy and Information are key concepts not only in Information Theory but also in Physics: historically in the fields of Thermodynamics, Statistical and Analytical Mechanics, and, more recently, in the field of Information Physics. In this paper we argue that Information Physics reconciles and generalizes statistical, geometric, and mechanistic views on information. We start by demonstrating how the use and interpretation of Entropy and Information coincide in Information Theory, Statistical Thermodynamics, and Analytical Mechanics, and how this can be taken advantage of when addressing Earth Science problems in general and hydrological problems in particular. In the second part we discuss how Information Physics provides ways to quantify Information and Entropy from fundamental physical principles. This extends their use to cases where the preconditions to calculate Entropy in the classical manner as an aggregate statistical measure are not met. Indeed, these preconditions are rarely met in the Earth Sciences due either to limited observations or the far-from-equilibrium nature of evolving systems. Information Physics therefore offers new opportunities for improving the treatment of Earth Science problems.

As pessoas são consideradas os motores de funcionamento e sucesso de uma organização. O investimento no seu desenvolvimento consiste numa prioridade para as organizações que procuram alcançar uma vantagem competitiva no mercado. Deste modo, a presente investigação incide sobre o estudo da relação entre as condições oferecidas pela organização com o objectivo de desenvolver as competências dos seus colaboradores, mais especificamente através da implementação de um novo modelo de avaliação de desempenho, e o reconhecimento deste apoio por parte dos colaboradores. Para tal, procedeu-se à construção do Questionário sobre Percepção de Apoio ao Desenvolvimento de Competências, que foi aplicado a uma amostra de 30 colaboradores de uma Empresa de Distribuição. Os resultados obtidos tendem a indicar uma percepção positiva por parte destes quanto ao apoio disponibilizado pela organização relativamente ao desenvolvimento de competências. No final são apresentados os contributos deste estudo a nível empírico e teórico, assim como as suas limitações, e sugestões para futuras linhas de investigação.

With climate change, drought is expected to increase, and its negative impacts will be particularly important in developing countries, usually with rainfall-dependent agriculture. The Cabo Verde archipelago is characterized by limited resources, remoteness, vulnerability to natural disasters, and a fragile environment. In this study, we provide the first report of the current status and trends of agriculture in Cabo Verde. We present data on the current performance of agricultural production areas in these islands and discuss them in terms of their most important natural constraint, water. Also, we assess the impact of institutional strategies on crop production and evaluate recent mechanisms that have been engaged towards agrarian development in this archipelago. Our results show that, among the ten Cabo Verde Islands, Santiago has the largest area used for agriculture (52.5%), followed by Santo Antão (16%) and Fogo (15.8%), and that rainfed farming dominates in all of them. The staple crops, such as maize and beans, are produced through rainfed subsistence farming, whereas irrigated crops (i.e., sugarcane, tomatoes) are mostly grown for commercial purposes. The prolonged drought periods, exposure, erosion and soil degradation, which led to increasing desertification over the last decades, have been identified as the main constraints to agrarian development across the ten islands of the archipelago. The strategies of Cabo Verde government to mitigate water scarcity through small-scale irrigation based mainly on small dams and drip irrigation technology have a marked effect on agricultural production in the predominantly arid and semi-arid areas of this archipelago.

Serendipita indica (former Piriformospora indica) is a non-obligate endophytic fungus and generally a plant growth and defence promoter with high potential to be used in agriculture. However, S. indica may switch from biotrophy to saprotrophy losing its plant growth promoting traits. Our aim was to understand if the free-living stage growth conditions (namely C availability) regulate S. indica’s phenotype, and its potential as plant-growth-promoting-microbe (PGPM). We grew S. indica in its free-living stage under increasing C availabilities (2–20 g L–1 of glucose or sucrose). We first characterised the effect of C availability during free-living stage growth on fungal phenotype: colonies growth and physiology (plasma membrane proton pumps, stable isotopic signatures, and potential extracellular decomposing enzymes). The effect of the C availability during the free-living stage of the PGPM was evaluated on wheat. We observed that C availability during the free-living stage regulated S. indica’s growth, ultrastructure and physiology, resulting in two distinct colony phenotypes: compact and explorer. The compact phenotype developed at low C, used peptone as the major C and N source, and displayed higher decomposing potential for C providing substrates; while the explorer phenotype developed at high C, used glucose and sucrose as major C sources and casein and yeast extract as major N sources, and displayed higher decomposing potential for N and P providing substrates. The C availability, or the C/N ratio, during the free-living stage left a legacy to the symbiosis stage, regulating S. indica’s potential to promote plant growth: wheat growth promotion by the explorer phenotype was ± 40% higher than that by the compact phenotype. Our study highlights the importance of considering microbial ecology in designing PGPM/biofertilizers. Further studies are needed to test the phenotypes under more extreme conditions, and to understand if the in vitro acquired characteristics persist under field conditions.

A new species of the miniaturised microhylid frog genus Stumpffia, from north-western Madagascar, is described. Stumpffia froschaueri sp. nov. differs from all other described Stumpffia species in colouration and morphology and is genetically divergent (≥ 7% uncorrected p-distance to all other nominal species of the genus) in a fragment of the mitochondrial 16S rRNA gene and in a segment of the nuclear Rag-1 gene. The new species is reliably known only from a few specimens collected in the Sahamalaza (and surroundings) region. Its known distribution is limited to three forest patches severely threatened by fire, drought and high levels of forest clearance, thus suggesting a classification of “Critically Endangered” according to IUCN Red List criteria.

Atmospheric nitrogen deposition and other sources of environmental eutrophication have increased substantially over the past century worldwide, notwithstanding the recent declining trends in Europe. Despite the recognized susceptibility of plants to eutrophication, few studies evaluated how impacts propagate to consumers, such as pollinators. Here we aim to test if soil eutrophication contributes to the temporal dynamics of pollinators and their larval resources. We used a temporally and spatially explicit historical dataset with information on species occurrences to test if soil eutrophication, and more specifically nitrogen deposition, contributes to the patterns of change of plant and pollinator richness in the Netherlands over an 80 yr period. We focus on bees and butterflies, two groups for which we have good knowledge of larval resources that allowed us to define groups of species with different nitrogen related diet preferences. For each group we estimated richness changes between different 20-yr periods at local, regional and national scale, using analytical methods developed for analyzing richness changes based on collection data. Our findings suggest that the impacts of soil eutrophication on plant communities propagate to higher trophic levels, but with a time-lag. Pollinators with nitrogen-related diet preferences were particularly affected, in turn potentially impairing the performance of pollinator-dependent plants. Pollinator declines continued even after their focal plants started to recover. In addition, our results suggest that current levels of nitrogen deposition still have a negative impact on most groups here analyzed, constraining richness recoveries and accentuating declines. Our results indicate that the global increase in nitrogen availability plays an important role in the ongoing pollinator decline. Consequently, species tolerances to soil nitrogen levels should be considered across all trophic levels in management plans that aim to halt biodiversity loss and enhance ecosystems services worldwide.

The genus Colletotrichum has witnessed tremendous variations over the years in the number of species recognized, ranging from 11 to several hundreds. Host-specific fungal species, once the rule, are now the exception, with polyphagous behavior regarded as normal in this genus. The species Colletotrichum kahawae was created to accommodate the pathogens that have the unique ability to infect green developing coffee berries causing the devastating Coffee Berry Disease in Africa, but its close phylogenetic relationship to a polyphagous group of fungi in the C. gloeosporioides species complex led some researchers to regard these pathogens as members of a wider species. In this work we combine pathological, morphological, cytogenomic, biochemical, and molecular data of a comprehensive set of phylogenetically-related isolates to show that the Coffee Berry Disease pathogen forms a separate species, C. kahawae, and also to assign the closely related fungi, previously in C. kahawae subsp. cigarro, to a new species, C. cigarro comb. et stat. nov. This taxonomic clarification provides an opportunity to link phylogeny and functional biology, and additionally enables a much-needed tool for plant pathology and agronomy, associating exclusively C. kahawae to the Coffee Berry Disease pathogen.