Repositório RCAAP

Procurou-se desenvolver um método para avaliar a resistência de mudas novas de cafeeiros ao nematóide Meloidogyne exigua. Dois experimentos foram realizados, a fim de testar a eficiência de quatro métodos de infestação, em mudas do cultivar mundo novo de Coffea arabica, utilizando-se areia como substrato, em caixas de plástico. A avaliação do ataque pelo nematóide foi realizada subjetivamente, dando-se um ponto na ausência de galhas e cinco pontos na presença de elevado número de galhas nas raízes do cafeeiro. Com base nos parâmetros estudados, verificou-se que 60 dias após a germinação das sementes, o sistema de infestação mais eficiente consistiu na colocação de pequenos pedaços de raízes de cafeeiro com galhas, misturados com o substrato das caixas de germinação, juntamente com uma suspensão em água, de ovos e larvas do nematóide. Em leitura efetuada 150 dias após a germinação, não se verificou diferença significativa entre a infestação provocada através do uso de pequenos pedaços de raízes e com a suspensão contendo ovos e larvas ou a associação de ambos os métodos de infestação. Em todos estes tratamentos notou-se que as raízes dos cafeeiros possuíam elevado número de galhas. Os dados obtidos indicam a possibilidade de realizar testes precoces de resistência ao nematóide M. exigua, o que é de particular interesse no plano geral de melhoramento visando resistência a esse parasita.

Foi estudada a associação da aplicação do regulador de crescimento CCC (Chlorocholine Chloride) e densidade de plantio, em algodoeiro. Em Campinas e Sumaré estudou-se o eleito da população de 4, 8 e 16 plantas /metro linear, com e sem CCC. Em Tietê e Piracicaba foram utilizadas 4, 8, 16 e 32 plantas /metro, sendo 16 e 32 com e sem CCC. Aplicou-se a dose de 50 gramas do ingrediente ativo do CCC/ha 50 a 70 dias após a emergência das plantas. Os resultados mostraram que a aplicação do CCC retardou o desenvolvimento vegetativo das plantas, determinando sensível diminuição no porte, aumentou a precocidade de abertura dos capulhos, diminuiu a porcentagem de fibra, havendo ainda melhoria no peso de capulho, peso de sementes e no comprimento das fibras. Não houve modificação na produção de algodão, como também na uniformidade de comprimento, índice Micronaire, resistência Pressley e maturidade das fibras. Ocorreu maior produção para tratamentos com maior densidade de plantas, comparativamente aos tratamentos com menor densidade. Embora não significativo estatisticamente, verificou-se que para maior número de plantas ocorreu aumento no comprimento das fibras e menor peso de capulho.

Sementes de algodão do cultivar IAC 13-1 foram deslintadas por quatro diferentes métodos, a saber: mecanicamente, por ácido sulfúrico, por gás clorídrico e por flambagem. Após seleção em mesa de gravidade, cada porção relativa a um determinado método de deslintamento foi dividida em duas, sendo uma delas tratada com fungicída PCNB 75%, nas dosagens recomendadas pelos fabricantes. Após o teste inicial de germinação, as sementes foram armazenadas em ambiente de laboratório e novos testes de germinação foram feitos aos 3, 6 e 12 meses. Em todos os períodos de armazenamento as porcentagens de germinação das sementes deslintadas por ácido sulfúrico e gás clorídrico foram superiores às das sementes deslintadas mecanicamente ou por flambagem que, por sua vez, foram iguais entre si ou com a flambagem ocupando posição intermediária. Em todos os períodos as porcentagens de germinação das sementes tratadas com fungicida foram superiores às das sementes sem esse tratamento.

Foram conduzidos estudos em vasos e no campo, para conhecer até que profundidade ocorria germinação de capim-carrapicho (Cenchrus echinatus L.). Em vasos esta ocorreu até 11 cm de profundidade e no campo até 9 cm em solo argiloso, e 10 cm em solo barrento. Foi frequente a germinação de duas ou mais cariopses por infrutescência. Em dois outros testes em vasos foi estudado o local de ação do trifluralin na dose de 0,84 kg/ha de i.a., em solo barrento. Verificou-se ação do herbicida nas zonas meristemáticas da raiz e do coleóptilo, causando inibição de desenvolvimento longitudinal do mesocótilo e da raiz. Na ponta da raiz primária, no primeiro nó e nó coleoptilar verificou-se aumento pronunciado de diâmetro.

São relatadas as variações anuais de produção de frutos e composição de folhas com 6-7 meses de idade, num ensaio fatorial NPK 3³ de longa duração com laranjeiras-baianinha, clone velho, sobre o porta-enxerto laranjeira-caipira. Para definir o fator hídrico foram empregados balanços hídricos para períodos bimestrais, sendo os resultados de cada ano correlacionados com os respectivos dados de análise foliar e de produção de laranja. Foram avaliados os efeitos da adubação na produção e determinadas várias correlações entre a concentração de nutrientes nas folhas e entre estes e a produção. A ausência de correlação entre os dados dos balanços hídricos meteorológicos (excedentes-deficiências) e a produção, e a fraca correlação entre os balanços hídricos e os teores de nutrientes nas folhas no conjunto dos anos do período estudado, indicaram que, nas condições do experimento, a irrigação suplementar não teria efeito sensível no aumento de produção da laranjeira. As respostas as adubações sobre a produção podem ser explicadas pelos aumentos dos teores de N, K e Ca das folhas e as correlações positivas verificadas entre esses elementos e a produção.

O presente trabalho foi desenvolvido visando à obtenção de linhagens de Crotalaria juncea autocompatíveis e resistentes à murcha de Ceratocystis fimbriata. Plantios sucessivos de crotalária comum foram efetuados em área altamente infestada pelo patógeno. Em cada ciclo foram selecionadas plantas que chegaram a produzir sementes, sendo estas plantadas no ano seguinte. Após quatro anos, o material resultante foi cruzado com linhagens autocompatíveis, visando reunir o caráter resistência à condição de autocompatibilidade. A geração F1 desse cruzamento foi retrocruzada com pais resistentes e com pais autocompatíveis. As populações assim obtidas foram levadas até a geração F4, sendo que em cada geração eram feitas inoculações e seleção de plantas resistentes e autocompatíveis. A geração F5, com 1.030 plantas, foi selecionada para produção e qualidade de sementes, resultando em 111 plantas com as características desejadas. As linhagens dessas plantas, após inoculação com isolados do fungo, mostraram alta porcentagem de plantas autocompatíveis, com resistência à murcha.

Procurou-se identificar fontes de resistência ao nematóide Meloidogyne exígua em cafeeiros derivados de híbridos interespecíficos e em progênies de café arábica oriundas da Etiópia, em experimentos estabelecidos em duas localidades. A infestação foi feita por dois processos usados com freqüência nesse tipo de trabalho. As avaliações do grau de ataque foram feitas quatro e 12 meses após a infestação, em Campinas e Ribeirão Preto, respectivamente, adotando-se escala variando de zero, para ausência de galhas, a cinco pontos para grande intensidade de ataque. Verificou-se que a leitura feita aos quatro meses é tão eficiente quanto aos 12 meses, indicando a possibilidade de abreviar consideravelmente a duração dessas avaliações. Notou-se, no experimento de Ribeirão Preto, uma redução de aproximadamente 8% no crescimento das mudas infestadas e verificou-se, também, uma redução de 11,5% e 12% nos pesos verde e seco das plantas, devido ao ataque do nematóide. De 1.692 plantas examinadas nos dois experimentos, selecionaram-se 106 (6,3%), caracterizadas pela ausência de galhas. As populações derivadas dos híbridos entre Coffea arabica e C. canephora revelaram-se mais promissoras, contribuindo com maior número de plantas resistentes. As plantas do experimento instalado em Campinas foram inoculadas também com a raça II de Hemileia vastatrix para o estudo da resistência conjunta aos dois patógenos. Essas seleções, em número de 38, representam valioso material para o programa de melhoramento do cafeeiro.

Plantas de cinco populações do café icatu foram classificadas em seis tipos de reação, de acordo com o grau de infecção pela H. vastatrix, sendo grau 1 correspondente a plantas resistentes e grau 6 a suscetíveis. Procurou-se determinar a relação entre a produtividade e os tipos de reação apresentados, bem como a variabilidade de produção em cada um desses tipos de reação. Verificou-se que não diferiram estatisticamente as médias de produção de plantas selecionadas correspondentes a cada tipo de reação ao ataque da ferrugem, dentro de quatro populações de icatu estudadas (H 4782-7, H 4782-10, H 4782-13 e H 3851-2), bem como entre essas populações. As plantas em cada população foram também agrupadas, de acordo com sua produção, em intervalos de 2kg, determinando-se a porcentagem de plantas dos vários tipos de reação ao patógeno nesses intervalos. Verificou-se que nos intervalos mais altos, a partir de 20kg, são mais freqüentes as plantas com reação dos tipos 1, 2 e 3, indicando que aquelas mais suscetíveis, dos tipos 5 e 6, possivelmente tiveram sua produtividade afetada pelo maior ataque da ferrugem. A variabilidade na produção total apresentada pelas plantas individuais das diferentes progênies do café icatu foi acentuada, dando margem à seleção de cafeeiros produtivos dentro dos diferentes tipos de reação ao ataque de H. vastatrix.

Foi estudado o efeito de temperaturas elevadas no desenvolvimento da ferrugem do cafeeiro, verificando-se que a exposição de plantas a temperatura próxima de 40ºC por quatro horas durante quatro dias foi suficiente para impedir a evolução da moléstia. Períodos menores foram ineficientes para afetar o desenvolvimento do fungo.

Estudou-se em condições de laboratório o comportamento, em relação ao Sitophilus zeamais Motschulsky 1855 (Coleoptera, Curculionidae), de milhos sintéticos (composto flint, composto dent. e centralmex), híbridos comerciais (Ag 152 e Hmd 7974), pipoca comercial (pirapoca) e um milho supostamente resistente (acre). Analisaram-se as correlações. existentes entre o comprimento da palha além da ponta da espiga, compacidade, dureza do grão, ataque de Heliothis zea Boddie, 1850 (Lepidoptera, Noctuidae), com o ataque de Sitophilus zeamais. Os resultados sugerem que o fator mais importante na resistência do milho-em-palha, a Sitophilus zeamais é a compacidade da mesma, vindo em segundo lugar o furo causado pela saída da lagarta Heliothis zea que atinge o solo para entrar no estádio de pupa.

Informações iniciais indicavam que o cafeeiro arábica comportava-se como planta de dias curtos. Crescimento vegetativo mais intenso e inibição de florescimento são a conseqüência de exposição a dias longos, superiores a 14 horas. Dados recentes demonstram que essa reação não é tão clara. No presente estudo são apresentados dados relativos a ensaios realizados visando estabelecer a reação de plantas dos cultivares mundo novo, catuaí-amarelo, burbom-vermelho, geisha e semperflorens a fotoperíodos de 12 e 18 horas. Os resultados indicaram que 18 horas de luz induzem crescimento mais intenso na altura e número de internódios. O florescimento não foi afetado pelo comprimento do dia, embora os cultivares tenham reagido com diferentes intensidades. É sugerido que o cafeeiro, uma vez induzido, independe de novos períodos de indução. O efeito parece permanecer por longo tempo. A idade dos tecidos parece ser elemento determinante na capacidade de indução e diferenciação das gemas florais.

ABSTRACT BACKGROUND: Inflammatory bowel diseases (IBD) are chronic inflammatory affections of recurrent nature whose incidence and prevalence rates have increased, including in Brazil. In long term, they are responsible for structural damage that impacts quality of life, morbidity and mortality of patients. OBJECTIVE: To describe the profile of physicians who treat IBD patients as well as the characteristics of IBD care, unmet demands and difficulties. METHODS: A questionnaire containing 17 items was prepared and sent to 286 physicians from 101 Brazilian cities across 21 states and the Federal District, selected from the register of the State Commission of the “Study Group of Inflammatory Bowel Disease of Brazil” (GEDIIB). RESULTS: The majority of the physicians who answered the questionnaire were gastroenterologists and colorectal surgeons. More than 60% had up to 20 years of experience in the specialty and 53.14% worked at three or more locations. Difficulties in accessing or releasing medicines were evident in this questionnaire, as was referrals to allied healthy professionals working in IBD-related fields. More than 75% of physicians reported difficulties in performing double-balloon enteroscopy and capsule endoscopy, and 67.8% reported difficulties in measuring calprotectin. With regard to the number of patients seen by each physician, it was shown that patients do not concentrate under the responsibility of few doctors. Infliximab and adalimumab were the most commonly used biological medicines and there was a higher prescription of 5-ASA derivatives for ulcerative colitis than for Crohn’s disease. Steroids were prescribed to a smaller proportion of patients in both diseases. The topics “biological therapy failure” and “new drugs” were reported as those with higher priority for discussion in medical congresses. In relation to possible differences among the country’s regions, physicians from the North region reported greater difficulty in accessing complementary exams while those from the Northeast region indicated greater difficulty in accessing or releasing medicines. CONCLUSION: The data obtained through this study demonstrate the profile of specialized medical care in IBD and are a useful tool for the implementation of government policies and for the Brazilian society as a whole.

ABSTRACT BACKGROUND: Electrogastrography (EGG) is a noninvasive technique for the assessment of gastric myoelectrical activity using electrodes placed on the abdominal surface. Changes in gastric myoelectrical activity may be associated with diseases such as gastroparesis, functional dyspepsia, nausea, and recurrent vomiting. In Brazil, no studies to date have assessed gastric myoelectrical activity using multichannel EGG in healthy individuals. OBJECTIVE: To establish normal values of transcutaneous multichannel EGG in healthy Brazilian individuals. METHODS: This was a prospective study including 20 healthy individuals who underwent EGG. Recording was performed during two periods: a preprandial recording was performed for 30 minutes, and a postprandial recording was performed for 30 minutes after a soft-solid meal of 400 kcal (20 grams of proteins, 60 grams of carbohydrates, and 9 grams of fat). RESULTS: We assessed dominant frequency (DF) parameters, %DF distribution, the instability coefficient, and the power ratio (PR). A total of 20 individuals (11 women and 9 men) with a mean age of 39.5±7.4 years were included. Mean DF (95%CI) ranged from 2.4 to 3.1 cpm in the resting phase and 2.6 to 3.2 cpm in the postprandial period. The %DF in normogastria range was >70% in all healthy individuals. We identified that only one individual did not present a positive response to the test meal, and the other 19 individuals showed a PR greater than 1. The instability coefficient did not change significantly with meal intake. CONCLUSION: Multichannel EGG may be applied in future studies to evaluate gastric motility disorders in the Brazilian population.

ABSTRACT BACKGROUND: There are quite a few studies examining prognostic factors in non-traumatic splenectomies compared to traumatic ones. OBJECTIVE: This study aimed to evaluate the predictors of mortality in patients who underwent splenectomy for non-traumatic spleen diseases. METHODS: Medical records of the patients, who had undergone total splenectomy for non-traumatic spleen diseases at a tertiary center between January 2009 and December 2019, were retrospectively reviewed. Exclusion criteria included patients younger than 18 years of age, partial splenectomies, splenectomies applied to facilitate surgery for malignancy on contiguous organs, and splenectomies performed during liver transplantation. Iatrogenic splenic injuries were regarded as trauma and these cases were also excluded. RESULTS: The current study included 98 patients. Nine (9.2%) patients died. In univariate analysis, age, the presence of hematological neoplasia, hematocrit, hemoglobin, white blood cell counts, neutrophil-to-lymphocyte ratio, indications for splenectomy, application of emergency surgery, surgical technique, and transfusion of blood components were all significantly associated with mortality. In multivariate analysis, the presence of hematological malignancy [P=0.072; OR=7.17; (CI: 0.386-61.56)], the application of emergency surgery [P=0.035; OR=8.33; (CI: 1.165-59.595)] and leukocytosis [P=0.057; OR=1.136; (CI: 0.996-1.296)] were found to be positively associated with mortality. CONCLUSION: Hematologic neoplasia, emergency surgery, and leukocytosis were the independent predictors of mortality in patients, who were operated on for non-traumatic spleen diseases. A thorough preoperative evaluation, early therapeutic intervention, and advanced surgical techniques are important and can serve to minimize complications and mortality in case of inevitable splenectomy. Immunological research can provide new therapeutic opportunities that may impact positively on patients by minimizing morbidity and mortality.

ABSTRACT BACKGROUND: The effectiveness of colonoscopy for colorectal cancer (CRC) screening depends on quality indicators, which adenoma detection rate (ADR) being the most important. Proximal serrated polyp detection rate (pSPDR) has been studied as a potential quality indicator for colonoscopy. OBJECTIVE: The aim is to analyze and compare the difference in ADR and pSPDR between patients undergoing screening colonoscopy and an unselected population with other indications for colonoscopy, including surveillance and diagnosis. METHODS: This is a historical cohort of patients who underwent colonoscopy in the digestive endoscopy service of a tertiary hospital. Out of 1554 colonoscopies performed, 573 patients were excluded. The remaining 981 patients were divided into two groups: patients undergoing screening colonoscopy (n=428; 43.6%); patients with other indications including surveillance and diagnosis (n=553; 56.4%). RESULTS: Adenoma detection rate of the group with other indications (50.6%) was higher than that of the screening group (44.6%; P=0.03). In regarding pSPDR, there was no difference between pSPDR in both groups (screening 13.6%; other indications 13.7%; P=0.931). There was no significant difference in the mean age (P=0.259) or in the proportion of men and women (P=0.211) between both groups. CONCLUSION: Proximal serrated polyp detection rate showed an insignificant difference between groups with different indications and could be used as a complementary indicator to adenoma detection rate. This could benefit colonoscopists with low colonoscopy volume or low volume of screening colonoscopies.

ABSTRACT BACKGROUND: Nonalcoholic fatty liver disease (NAFLD) is one of the most common forms of chronic liver disease worldwide. Approximately 20% of individuals with NAFLD develop nonalcoholic steatohepatitis (NASH), which is associated with increased risk of cirrhosis, portal hypertension, and hepatocellular carcinoma. Intestinal microflora, including small intestinal bacterial overgrowth (SIBO), appear to play an important role in the pathogenesis of the disease, as demonstrated in several clinical and experimental studies, by altering intestinal permeability and allowing bacterial endotoxins to enter the circulation. OBJECTIVE: To determine the relationship between SIBO and endotoxin serum levels with clinical, laboratory, and histopathological aspects of NAFLD and the relationship between SIBO and endotoxin serum levels before and after antibiotic therapy. METHODS: Adult patients with a histological diagnosis of NAFLD, without cirrhosis were included. A comprehensive biochemistry panel, lactulose breath test (for diagnosis of SIBO), and serum endotoxin measurement (chromogenic LAL assay) were performed. SIBO was treated with metronidazole 250 mg q8h for 10 days and refractory cases were given ciprofloxacin 500 mg q12h for 10 days. RESULTS: Overall, 42 patients with a histopathological diagnosis of NAFLD were examined. The prevalence of SIBO was 26.2%. Comparison of demographic and biochemical parameters between patients with SIBO and those without SIBO revealed no statistically significant differences, except for use of proton pump inhibitors, which was significantly more frequent in patients with positive breath testing. The presence of SIBO was also associated with greater severity of hepatocellular ballooning on liver biopsy. Although the sample, as a whole, have elevated circulating endotoxin levels, we found no significant differences in this parameter between the groups with and without SIBO. Endotoxin values before and after antibiotic treatment did not differ, even on paired analysis, suggesting absence of any relationship between these factors. Serum endotoxin levels were inversely correlated with HDL levels, and directly correlated with triglyceride levels. CONCLUSION: Serum endotoxin levels did not differ between patients with and without SIBO, nor did these levels change after antibacterial therapy, virtually ruling out the possibility that elevated endotoxinemia in non-cirrhotic patients with NAFLD is associated with SIBO. Presence of SIBO was associated with greater severity of ballooning degeneration on liver biopsy, but not with a significantly higher prevalence of NASH. Additional studies are needed to evaluate the reproducibility and importance of this finding in patients with NAFLD and SIBO.

ABSTRACT BACKGROUND: Endoscopic submucosal dissection (ESD) enables en bloc excision of superficial neoplasms. Although ESD is widely practiced in Eastern countries like Japan, South Korea and China, its use in the West is supposed to be still limited to few tertiary centers. OBJECTIVE: This study aimed to investigate the clinical practice of ESD in Brazil by means of an electronic questionnaire elaborated by the Brazilian Society of Digestive Endoscopy (SOBED). METHODS: In October 2019, 3512 endoscopist members of SOBED were invited to respond to an electronic survey containing 40 questions divided into four topics: operator profile; clinical experience with ESD; adverse events and training. Informed consent was requested to all participants. The investigators reviewed all responses and considered valid if more than 50% of the questions were answered and additional information, when requested, was provided. RESULTS: A total of 155 (4.4%) qualified responses entered the study. ESD has been practiced in 22 of 26 Federation States and majority of respondents (32.2%) have 10 to 20 years of practice. ESD has been performed more frequently in private hospitals (101 individuals, 66.9%) and private ambulatory centers (41 individuals, 27.1%). ESD was performed mainly in the stomach (72%), followed by the rectum (57%) and 80% of the individuals reported less than 25 operations. Twenty-four (15.4 %) individuals reported perforation and 4 (2.5%) of them reported postoperative deaths. Approximately a quarter of responders denied hands-on training in models or visit to training centers. CONCLUSION: ESD appears to be practiced throughout the country, not only in tertiary or academic institutions and mainly in private practice. Most operators received limited training and still are at the beginning of their learning curve. The reported adverse events and mortality rates appear to be higher than Eastern reports.

ABSTRACT BACKGROUND: Abdominal wall hernia is one of the most common surgical pathologies. The advent of minimally invasive surgery raised questions about the best technique to be applied, considering the possibility of reducing postoperative pain, a lower rate of complications, and early return to usual activities. OBJECTIVE: To evaluate the frequency of open and laparoscopic hernioplasties in Brazil from 2008 to 2018, analyzing the rates of urgent and elective surgeries, mortality, costs, and the impact of laparoscopic surgical training on the public health system. METHODS: Nationwide data from 2008 to 2018 were obtained from the public health registry database (DATASUS) for a descriptive analysis of the selected data and parameters. RESULTS: 2,671,347 hernioplasties were performed in the period, an average of 242,850 surgeries per year (99.4% open, 0.6% laparoscopic). The economically active population (aged 20-59) constituted the dominant group (54.5%). There was a significant reduction (P<0.01) in open surgeries, without a compensatory increase in laparoscopic procedures. 22.3% of surgeries were urgent, with a significant increase in mortality when compared to elective surgeries (P<0.01). The distribution of laparoscopic surgery varied widely, directly associated with the number of digestive surgeons. CONCLUSION: This study presents nationwide data on hernia repair surgeries in Brazil for the first time. Minimally invasive techniques represent a minor portion of hernioplasties. Urgent surgeries represent a high percentage when compared to other countries, with increased mortality. The data reinforce the need for improvement in the offer of services, specialized training, and equalization in the distribution of procedures in all regions.

BACKGROUND: Biological therapy and new drugs have revolutionized the treatment of inflammatory bowel disease. Ideally, the choice of medication should be a shared decision with the patient, aiming at greater satisfaction, compliance, and consequently, favorable clinical outcome. OBJECTIVE: This study aims to evaluate patient’s preferences in the choice of their therapy and the factors that influence this choice. METHODS: This cross-sectional study enrolled 101 outpatients with Crohn’s disease or ulcerative colitis. The inclusion criteria were age ≥18 years and no previous exposure to biological therapy. Patients’ preferences were assessed through questions that addressed the preferred mode of administration (oral, subcutaneous, or intravenous) and the factors that determined the choice of medication (efficacy, medical indication, fear of medication, convenience, mode of application, and personal doctors’ indication). RESULTS: The mean age was 43.6±13.5 years, 75.3% were female, and 81.2% were cases of ulcerative colitis. Regarding the mode of administration, the majority of patients preferred oral (87.1%), followed by intravenous (6.93%) and subcutaneous (5.94%) medications. The reasons were “I prefer to take it at home” (42.57%), “I have more freedom” (36.63%), “I don’t like self-application” (29.70%), and “I believe it works better” (19.80%). Younger patients and patients in clinical disease activity preferred intravenous mode compared to the oral route (P<0.05). Doctor’s opinion (98%) was an important factor associated with the medication choice. CONCLUSION: Oral route was the preferred mode of administration and most patients took their physician’s opinion into account in their choice of medication.

ABSTRACT BACKGROUND: Lubiprostone is a type 2 chloride channel activator that has been shown to be efficacious and safe in the treatment for chronic constipation. OBJECTIVE: To systematically review randomized clinical trials (RCTs) assessing efficacy of lubiprostone for patients with chronic idiopathic constipation (CIC), irritable bowel syndrome with predominant constipation (IBS-C) and opioid-induced constipation (OIC). METHODS: Searches were conducted in PubMed, LILACS, Cochrane Collaboration Database, and Centre for Reviews and Dissemination. Lubiprostone RCTs reporting outcomes of spontaneous bowel movements (SBM) and abdominal pain or discomfort were deemed eligible. Meta-analysis was performed calculating risk ratios and 95% confidence intervals, using the Mantel-Haenszel method and random effects model. RESULTS: Searches yielded 109 records representing 93 non-duplicate publications, and 11 RCTs (978 CIC, 1,366 IBS-C, 1,300 OIC, total = 3,644) met inclusion criteria. Qualitative synthesis showed that for CIC patients, lubiprostone is superior to placebo in terms of SBM outcomes. Meta-analysis for CIC was feasible for full responder and SBM within 24h rates, indicating superiority of lubiprostone over placebo. For IBS-C, lubiprostone was significantly superior for all SBM outcomes in follow-ups ranging from 1 week-3 months. In terms of abdominal pain, lubiprostone provided significantly better symptoms relief, particularly after 1 month of treatment. For OIC, lubiprostone was more effective than placebo for both SBM and discomfort measures. CONCLUSION: Our findings demonstrated that lubiprostone is superior to placebo in terms of SBM frequency for CIC, IBS-C and OIC. In terms of abdominal symptoms, the most pronounced effect was seen for abdominal pain in IBS-C patients.