RCAAP Repository

Proporções de volumoso e concentrado associadas a ofertas hídricas para ovinos confinados

The effects of different offers roughage, concentrate and water performance, economic viability, characteristics and carcass yield of sheep were evaluated. We used 40 crossbred sheep de Santa Inês, uncastrated, with an average initial weight of 18.85 ± 2.80 kg and mean age 5.0 ± 2.0 months, distributed in a completely randomized design, on a 2x2 factorial design, consisting of two forage to concentrate ratios: 30:70 and 70:30 with their energy levels: 2.67 and 2.10 Mcal of ME/kg DM and two offerings of water (water ad libitum and restricted by 50%) with 10 replicates during 73 days. To evaluate the characteristics and performance of the carcasses, the animals were slaughtered at an average weight of 27.62 ± 3.06 kg. Proximate composition (% moisture, % ash, % protein and % ether extract) of Longissimus dorsi was determined, and the same was considered the color parameters (L *, a * and b *), shear force, cooking loss and pH. The water restriction caused effect (P<0.05) on dry matter and nutrients, and total daily gain weight regardless of roughage and concentrate. There was no effect (P>0.05) of water restriction on feed conversion and efficiency. The total weight gain, average daily gain, feed conversion and efficiency were influenced by the proportions of forage and concentrate. The evaluation of economic results revealed that all combinations of water supply and levels of dietary energy to produce crossbred sheep de Santa Inês were positive for gross income and profit. Higher total cost of the diet was obtained when the animals were fed with high level of concentrate. There was no effect (P<0.05) of water restriction on the chemical composition, in variations of color (L *, a * and b *), weight and cut yields, and the evaluation parameters for carcass yield, except to the final weight. Weights and carcass were influenced (P<0.05) the proportion of forage and concentrate, no differences in cooling losses and biological yield. There was effect (P<0.05) of restriction of water in cooking loss. It was concluded that, when associated higher proportion of roughage in the diet with water restriction animal performance is reduced and the combination of diet and fluid restriction and concentrate more than 50% of consumption allows higher net income and improved benefit cost ratio. Higher weights and yields of hot and cold carcass are obtained in animals fed diets with a higher share of the concentrate.

Year

2022-12-06T23:45:52Z

Creators

Silva, Claudete Maria da

Determinação do segundo aminoácido limitante para codornas japonesas

This study aimed to determine the second limiting amino acid for Japanese quails fed with corn and soybean meal. Were used 1296 Japanese quail, females, divided into three experiments. The first experiment was conducted with birds aged 1-21 days, the second experiment with birds of 22-42 days and the experiment III with birds laying phase. The treatments in the three experiments consisted of reduction of crude protein in seven percentage points, approximately, with supplementation of the DL-methionine plus an L-amino acid essential: T1 (Met), T2 (T1+Lys), T3 (T1+Thr) and T4 (T1+Trp), T5 (T1+Val), T6 (T1+Arg), T7 (T1+Ile) and T8 (All). The parameters evaluated were feed intake (g/bird), weight gain (g/bird), feed conversion (g/g), laying rate (%), average egg weight (g), egg mass (g ), feed conversion per egg mass (kg feed/kg egg), feed conversion per dozen eggs (kg feed/dozen eggs), average weight of constituents (yolk, albumen and shell) eggs (g) , bark thickness (mm) and specific gravity (g/cm³). The statistical design was a completely randomized and the test for comparison of means was SNK at 5% probability. For performance results of 1-21 and 22-42 days, the weight gain and feed conversion were affected by amino acid supplementation. In the laying phase, feed intake, egg production, egg mass, and mass conversion per dozen, yolk weight and albumen weight were affected. We conclude that the second limiting amino acid for Japanese quails in phases 1-21 and 22-42 days, is lysine. During the laying period, it was not possible to determine the second limiting amino acid, meeting the requirements of methionine + cystine, lysine, threonine, tryptophan, valine, arginine and isoleucine digestible to obtain better results is required.

Year

2022-12-06T23:45:52Z

Creators

Cavalcante, Danilo Teixeira

Álgebra de Clifford: classificações e representações

In this paper, we study Clifford algebras so universal and constructive as quantization of exterior algebra, we classify all Clifford algebras associated with the quadratic Minkowski spaces (Rp+q, p,q), where p,q(u) = u21 +...+u2 p −(u2 p+1 +...+u2 p+q), u = (u1, ..., up+q) ∈ Rp+q, which we denote by Clp,q, as well as their complexifications. To do so, we use important results as the periodicity theorem Carton / Bott. The, we study their representations, emphasizing the Twisted Adjoint Representataion, Spin Representation and the Spin-Half Representation moreover using the number of Radon-Hurwitz we study representations of the algebras Cl0,k.

Year

2022-12-06T23:47:14Z

Creators

Farias, José Ginaldo de Sousa

Números Mórficos

Morphic numbers are numbers related to the form and, somehow, they establish a conception of beauty, aesthetics and harmony. These numbers have important of applications in various branches of knowledge, such as geometry, arithmetic, architecture, and engineering. There are only two morphic numbers, the golden number and the plastic number. The rst one has been studied since ancient Greece, and the second one has only become a subject of interest in the twentieth century, what makes the plastic number a relatively new branch of research. In this work, we will analyze a data collection concerning arithmetic, algebraic or geometric properties of these numbers, by establishing a straight relation between the morphic numbers and the Fibonacci and Padovan sequences.

Year

2022-12-06T23:47:14Z

Creators

Ferreira, Ronaebson de Carvalho

Da morte biológica a morte cultural: Um estudo sobre o morrer em casa em João Pessoa-PB

Ce travail envisage une étude anthropologique sur Ia pratique de"mourir a la maison", identifiée comme une attitude devant la mop, a Ia yule de joAo Pessoa. En accord avec les orientations théoriques sur les différentes systêmes culturels de mort chez Hertz (1978), Mauss, (1974) et Thomas (1993), on se rend compte de la mon et les rites flineraires en ernies de son caractere eollectif et symbolique qu'orientent aussi bien des conduités que la circulation de références sur lethéme. Appuyés par une recherche documentale, nous avons essayé ensuite de cueillir et évaluer, a travers d'entrevues ouvertes aupres des familiers des décédés, les motifs détérminants de Ia decision de mourir "a Ia maison". Ce choix a été permis aux sujets a travers des notions de réaliser a un niveau pratique une réprésentations de "la bonne mott",rendant au moribond ce qu"etait hors du cycle naturel de Ia vie: mourir "chez soi'!. D'autre part, on envisage un systême de classification de (a mort soutenu par des réprésentations sur Ia maladie, ayant comme résultat cette pratique significative qui met ensemblel'espace et le moment de la mon. Les rites funéraires ont devoilé des éléments du domaine de la convenience de la famille et du pragmatisme du monde urbain.

Year

2022-12-06T23:47:30Z

Creators

Maciel Neves, Ednalva

Poliedros Regulares no Ensino Médio

In this work we present a study of the regular polyhedra, comparing and discussing the concepts and de nitions given in the study of regular polyhedra in textbooks most widely used in Brazilian high schools. We prove the theorem of Euler, we calculate surface areas and volumes of regular polyhedra. Finally, we present some mathematical software that can be used by students and mathematics teachers in the spatial geometry classes as auxiliary material in the teaching and learning of this subject in the classroom.

Year

2022-12-06T23:46:10Z

Creators

Silva, Hércules do Nascimento

Limites de escala em modelos de armadilhas

Let X = fX 0;X0 = 0g be a mean zero -stable random walk on Z with inhomogeneous jump rates f 1 i ; i 2 Zg, with 2 (1; 2] and f i : i 2 Zg is a family of independent random walk variables with common marginal distribution in the basis of attraction of an -stable law with 2 (0; 2]. In this paper we derive results about the long time behavior of this process, we obtain the scaling limit. To this end, rst we will approach probability on metric spaces, speci cally treat the D space of the functions that are right-continuous and have left-hand limits. We will also expose some results dealing with stable laws that are directly related to the above problem.

Year

2022-12-06T23:47:14Z

Creators

Santos, Lucas Araújo

Diagnóstico socioambiental e intervenção educativa no semiárido paraibano: o estudo de caso do Bairro Jardim Sant'ana, Sousa-PB

Cultural changes are a challenge to the reduction of environmental impacts and to the ideal of coexistence with the semiarid especially on the urban zones, where have a greater detachment with relation to the environment of semiarid. Promote non-formal education in this region it is of great value to the construction of a new environmental culture. This study had as objective to diagnose the main socio-environmental problems of the Jardim Sant'ana neighborhood, in Sousa, semiarid of Paraíba, and assess the population perception in relation to the environmental culture, well as promote educational interventions focusing on critical environmental education for the children of that neighborhood. In the first part of the work it is presents the socio-environmental diagnostic that was elaborated, which is an important tool to support the development of public policies of coexistence with the semiarid region. After, the results of the educational intervention, the project Ser-Tão Ambiental, are presented and analyzed. The methodology of qualitative and quantitative type was adopted using theoretical assumptions of phenomenological research and participant observation. From the analysis and conclusion of research it was evident the urgent need of connection between the population and the paraiban semiarid. The Public Power it is the fundamental actor in this construction that needs effective public policies to turn it possible. It was also detected by means of project Ser- Tão Ambiental the importance of non-formal education promotion to the ecological citizen formation and to improve in the life quality of population. So, the studies and interventions realized in this research, results in an approximation of those involved with the environment they live in although primarily, important step in a construction of a new behavior, sensitizing the involved about their ecological role.

Year

2022-12-06T23:46:27Z

Creators

Portela, Lívia Feijó

A representação da escravidão nos Contos de Machado de Assis

This work aims to study the theme of slavery in the tales written by Machado de Assis, having as corpus the analyses of the stories “Pai contra mãe”, “O caso da vara” and “Mariana”, which are meaningful narratives about enslavement, as they represent, by the realistic form, the oppression promoted by the enslaver society on the black people. In this case, we selected three analytical categories to organize our research: the speech of the narrator; the characterizing of the characters; and the representation of black men and women in social relations, established by the dominant classes in Brazil in the nineteenth century. The objectives of this research are: establishing an interchange between the aesthetical production and the social environment in the work of Machado de Assis; exploring the racial power relations in the nineteenth century which are represented in stories; analyzing the dialectical relationship between the formal resources of Machadian text and black stereotypes in the slave society of the nineteenth century. Amidst the types of studies in the scientific methodology, our research is characterized as bibliographical. The theoretical background presented in our research was composed by the following research areas: about the nature of literary language, we approached the contribution of the Russian formalist studies and dialectics in the composition process, proposed by Antonio Candido and Anatol Rosenfeld; about the historical role of the black and the slave system in Brazil, we had as main reference the studies from “Escravismo Colonial”, by Jacob Gorender. By the analyzes that we realized, we reached to the main conclusion that the issue of slavery of blacks in Brazil materializes, by the aesthetic and formal features of literary language and thereby representing some slaveholders contexts, by bias of irony, it leaves an ambivalence between the legitimation and criticism of the Brazilian slave system during the nineteenth century.

Year

2022-12-06T23:46:59Z

Creators

França Neto, João Irineu de

Controlabilidade para alguns modelos da mecânica dos fluidos

The aim of this thesis is to present some controllability results for some fluid mechanic models. More precisely, we will prove the existence of controls that steer the solution of our system from a prescribed initial state to a desired final state at a given positive time. The two first Chapters deal with the controllability of the Burgers-α and Leray-α models. The Leray-α model is a regularized variant of the Navier-Stokes system (α is a small positive parameter), that can also be viewed as a model for turbulent flows; the Burgers-α model can be viewed as a related toy model of Leray-α. We prove that the Leray-α and Burgers-α models are locally null controllable, with controls uniformly bounded in α. We also prove that, if the initial data are sufficiently small, the pair state-control (that steers the solution to zero) for the Leray-α system (resp. the Burgers-α system) converges as α → 0+ to a pair state-control(that steers the solution to zero) for the Navier-Stokes equations (resp. the Burgers equation). The third Chapter is devoted to the boundary controllability of inviscid incompressible fluids for which thermal effects are important. They will be modeled through the so called Boussinesq approximation. In the zero heat diffusion case, by adapting and extending some ideas from J.-M. Coron [14] and O. Glass [45], we establish the simultaneous global exact controllability of the velocity field and the temperature for 2D and 3D flows. When the heat diffusion coefficient is positive, we present some additional results concerning exact controllability for the velocity field and local null controllability of the temperature. In the last Chapter, we prove the local exact controllability to the trajectories for a coupled system of the Boussinesq kind, with a reduced number of controls. In the state system, the unknowns are: the velocity field and pressure of the fluid (y, p), the temperature θ and an additional variable c that can be viewed as the concentration of a contaminant solute. We prove several results, that essentially show that it is sufficient to act locally in space on the equations satisfied by θ and c.

Year

2022-12-06T23:47:14Z

Creators

Souza, Diego Araújo de

Sobre o teoremas de Bohnenblurt - Hille

Os teoremas de Bohnenblust Hille, demonstrados em 1931 no prestigioso jornal Annals of Mathematics, foram utilizados como ferramentas muito úteis na solução do famoso Problema da convergência absoluta de Bohr. Após um longo tempo esquecidos, estes teoremas têm sido bastante explorados nos últimos anos. Este último quinquê- nio experimentou o surgimento de várias obras dedicadas a estimar as constantes de Bohnenblust Hille ([13, 18, 20, 26, 27, 39, 42, 44, 46, 53]) e também conexões inesperadas com a Teoria da Informação Quântica apareceram (ver, por exemplo, [38]). Há, de fato, quatro casos para serem investigados: polinomial (casos real e complexo) e multilinear (casos real e complexo). Podemos resumir em uma frase as principais informa ções dos trabalhos recentes: as constantes das desigualdades de Bohnenblust Hille são, em geral, extraordinariamente menores do que as primeiras estimativas tinham previsto. Neste trabalho apresentamos algumas das nossas pequenas contribuições ao estudo das constantes nas desigualdades de Bohnenblust-Hille, os quais encontram-se contidos em ([40, 41, 42, 44]).The Bohnenblust Hille theorems, proved in 1931 in the prestigious journal Annals of Mathematics, were used as very useful tools in the solution of the famous "Bohr's absolute convergence problem". After a long time overlooked, these theorems have been explored in the recent years. Last quinquennium experienced the rising of several works dedicated to estimate the Bohnenblust Hille constants ([13, 18, 20, 26, 27, 39, 42, 44, 46, 53]) and also unexpected connections with Quantum Information Theory appeared (see, e.g., [38]). There are in fact four cases to be investigated: polynomial (real and complex cases) and multilinear (real and complex cases). We can summarize in a sentence the main information from the recent preprints: the Bohnenblust Hille constants are, in general, extraordinarily smaller than the rst estimates predicted. In this work, we present some of our small contributions to the study of the constants of the inequalities Bohnenblust-Hille, these are contained in ([40, 41, 42, 44]).

Year

2022-12-06T23:47:30Z

Creators

Alarcón, Daniel Núnez

Sobre as extensões multilineares dos operadores absolutamente somantes

In this work we study two generalizations of the well-known concept of absolutely summing operators. The rst one consists of the multiple summing multilinear operators and it is focused on a result of coincidence that is equivalent to the Bohnenblust- Hille inequality. This inequality asserts that, for K = R or C and every positive integer m there exists positive scalars BK;m 1 such that N X i1;:::;im=1 U(ei1 ; : : : ; eim) 2m m+1!m+1 2m BK;m sup z1;:::;zm2DN jU(z1; :::; zm)j for every m-linear mapping U : KN KN ! K and every positive integer N, where (ei)N i=1 denotes the canonical basis of KN: In this line our main goal is the investigation of the best constants BK;m satisfying the above inequality. The second generalization involves the concept of absolutely summing multilinear operators at a given point; we present an abstract version of these operators involving many of their properties. We prove that, considering appropriate sequence spaces, we have other kind of operators as particular cases of our version.

Year

2022-12-06T23:47:14Z

Creators

Radrígues, Diana Marcela Serrano

Curvas de Bézier

In this work we will make an introduction an important application mathematics called Bézier curves. The history of this curve originated in industry automobile French , and found many applications in various elds of science. Revisit some concepts such as parametric functions, polynomials Bernstein and interpolation for de nition the curves Bézier. We will discuss the algorithm Casteljau which facilitates the construction of the curve and determine derivative. Throughout the text we will implement some examples with Geogebra software and LATEX in addition to discuss relevant issues that arouse public interest.

Year

2022-12-06T23:46:10Z

Creators

Almeida, Evert Elvis Batista de

Controle Estoc astico, Backward SDEs e EDPs Parab olicas

The Dissertation study the relations between control theory, stochastic calculus and parabolic partial di erential equations. The aim is to study representations of viscosity solutions for parabolic equations via the Feynman-Kac nonlinear formulas. To this end, the control theory plays an important role in the connection between the stochastic and deterministic approaches.

Year

2022-12-06T23:47:14Z

Creators

Nascimento, Jorge Alexandre Cardoso do

Soluções clássicas para uma equação elíptica semilinear não homogênea

This work is mainly concerned with the existence and nonexistence of classical solution to the nonhomogeneous semilinear equation Δu + up + f(x) = 0 in Rn, u > 0 in Rn, when n 3, where f 0 is a Hölder continuous function. The nonexistence of classical solution is established when 1 < p n=(n 2). For p > n=(n 2) there may be both existence and nonexistence results depending on the asymptotic behavior of f at infinity. The existence results were obtained by employed sub and supersolutions techniques and fixed point theorem. For the nonexistence of classical solution we used a priori integral estimates obtained via averaging.

Year

2022-12-06T23:47:14Z

Creators

Rocha, Suelen de Souza

Análise Metabolômica de Peperomia obtusifolia (L.) A. Dietr.

The species Peperomia obtusifolia (L.) A. Dietr, which belongs to the Piperaceae family, is a well-known plant, distributed from Mexico to South America. Previous phytochemical studies demonstrated the presence of compounds from several classes, such as: lignans, flavonoids, amides, phenolic compounds and chromenes, which demonstrated interesting biological activities. This work describes the metabolomic analysis of Peperomia obtusifolia, which study in real time, the metabolism of this species in many kinds of scenarios. However, to accomplish this study, firstly it was developed a standard protocol, capable of preparing the samples in a reproducible way, enabling the following metabolomic studies to generate reliable results. Later, it was conducted the metabolomic study of Peperomia obtusifolia and its varieties, by fingerprinting analysis, in order to characterize the metabolomic profiles of each one of them. Furthermore, it was also evaluated the behavior of the species against the stress induced by the herbivory of Monoplatus sp. In general, the analyzes were conducted by NMR of 1H (which the data was interpreted by PCA), mass spectrometry, HPLC, GC and its hyphenated techniques. As a result, it was found that, despite the fact that the Peperomia obtusifolia varieties belong to the same species, they had different metabolomes. Regarding the study of the biotic stress against the plant, changes were detected on the metabolomic profile of the attacked plants, indicating a response against the herbivore attack. As a major result of this research, it can be highlighted the fact that the same changes were also detected on the metabolome of the intact plants closed to the attacked ones, indicating a sort of interplant communication by volatile compounds, capable to induce the neighborhood yet not attacked, to be prepared for an imminent attack, giving them a better chance of survival.

Year

2022-12-06T23:47:14Z

Creators

Souto, Augusto Lopes

Avaliação da toxicidade e atividade antitumoral de nanopartículas de óxido de cério associados ao óxido de zinco

Nanomedicine is the use of nanomaterials in developing new therapeutic and diagnostic procedures. However, despite the advantages and different applications of nanoparticles, they also have potential toxic effects that should be properly assessed before use. Metal oxides and, in particular, metal oxide nanoparticles have many applications. Among them, cerium oxide nanoparticles and zinc oxide have shown different pharmacological activities, both alone, and in combination with each other. Cancer is a complex genetic disease which is a major public health problem worldwide, one of the leading causes of death in Brazil. This study aimed to evaluate the toxicity and antitumor activity in vivo of cerium oxide nanoparticles and zinc oxide association (NCZ). Initially it was evaluated the acute preclinical toxicity orally with estimated NCZ LD50 above 2000 mg/kg, therefore, the sample is considered to have low toxicity in our experimental conditions. Subsequently it was performed the evaluation of repeated doses toxicity study (28 days) of NCZ by oral route. The treatment resulted in a significant decrease in the consumption of water and feed, significant increase in enzyme activity of ALT and AST, significant decrease of urea, significant increase in the total white blood cell count, significant decrease in thymus index, significantly increased of ambulation and a decrease in time of self-cleaning and withdrawals. The study of acute toxicity by intraperitoneal route, the LD50 of NCZ was estimated at about 300 mg/kg enabling the choice of doses to be used in the study of antitumor activity. NCZ showed significant antitumor activity in Ehrlich ascites carcinoma model (EAC) at doses of 10, 20 and 40 mg/kg, considering the mass, tumor volume and total cell parameters. In the investigation of its mechanism of action was observed that NCZ increased the percentage of cells in sub-G1 phase, decreased microvessel peritoneal vascular, as well promoted an increased in the concentration of IL-1, IL-10, TNF- and IFN-. It was also evaluated the toxicity of NCZ in animals with EAC submitted to antitumor treatment for nine days. It was observed a significant decrease in feed intake, significant increase in ALT activity, significant decrease in serum urea concentration, significant increase in serum creatinine and significant increase in the mean corpuscular hemoglobin. According to the results, we can infer that NCZ has low toxicity and significant antitumor activity in vivo with mechanism of action probably related to interference with the progression of the cell cycle, inducing apoptosis, increased immune response and antiangiogenic action.

Year

2022-12-06T23:47:14Z

Creators

Xavier, Aline Lira

Avaliação dos efeitos farmacológicos e toxicológicos do estrato etanólico, fase clorofórmica e flavonoide de Praxelis clematidea (griseb.) R.M. King & H. Robinson (Asteraceae)

The increasing resistance of micro-oganismos pathogens to existing antimicrobial market has driven the search for new therapeutic alternatives, such as natural herbal products belonging to various families of the plant kingdom, such as Asteraceae, which are presented as a viable solution due to the low cost and easy access of the population. However, the search for compounds derived from plants with pharmacological property must always be attached to toxicological studies in order to show the absence of these substances harm to the human body. Based on these premissias, pharmacological and toxicological effects of the ethanol extract (EPC), chloroform phase (FPC) and 5,7,4'-trimethoxyflavone (TMF) from Praxelis clematidea were studied. For the realization of in vitro antimicrobials studies was used the microdilution test with different fungal and bacterial strains. In carrying out studies of antioxidant activity and cytotoxicity was used human erythrocytes obtained from the blood bank of the University Hospital Lauro Wanderley. In acute toxicity studies and genotoxicity was used Swiss mice derived from the Vivarium Thomas George / UFPB. The experiments of antimicrobial activity revealed that EPC and FPC promoted an antifungal effect against Candida species, suggesting that both have secondary metabolites active against fungi. The TMF, major compound isolated from FPC, promoted an antibacterial effect against gram positive and gram negative species, with minimum inhibitory concentration (MIC) of 128 ug / mL and antifungal effect against different Candida species. The MIC TMF was 32 ug / ml for the strains of Candida krusei. With regard to the strains of Candida albicans, both the MIC and the minimum fungicidal concentration (MFC) was 64 ug / mL, combined with it, this involved the action antifungal effect on the cell wall and the plasma membrane of this species studied fungus. In addition, TMF caused decreased erythrocyte damage both by the exposure of hydrogen peroxide, as the osmotic change and showed an antioxidant effect against methemoglobin formation in erythrocytes. The TMF showed low theoretical toxicity and good oral bioavailability by in silico analysis. These data were confirmed by analyzing the cytotoxicity against erythrocytes, which showed hemolysis values below 10% for all blood types tested. In the evaluation of acute toxicity after oral administration of TMF (300 mg/kg), it can be seen that the test compound did not induce behavioral changes in mice and only alter feed intake of animals treated without altering body weight, organ weights and parameters biochemical and hematological evaluated. The analysis of the genotoxic potential of TMF showed that this metabolite was not able to damage the DNA of cells of the peripheral blood of treated animals. In conclusion, these results suggest that the EPC FPC and TMF have antimicrobial effect and which also has the flavonoid antioxidant effect with low cytotoxicity potency, toxicology and genotoxicity.

Year

2022-12-06T23:47:14Z

Creators

Oliveira Filho, Abrahão Alves de

O carvacrol reduz a pressão arterial via ativação de canais receptores de potencial transiente em ratos espontaneamente hipertensos

TRP channels have been extensively studied in many physiological and pathological processes involved in blood pressure regulation. Carvacrol is well known to act on TRP channels in the vasculature, however there are no studies of its effects in hypertensive rats. Our aim was to evaluate the contribution of TRP channels in hypertension and evaluate the effects of carvacrol on TRP channels of SHR. In an electrophysiological approach, carvacrol (300 μM) inhibited the barium current, suggesting a reduction of calcium influx through L-type voltage-operated Ca2+ channels. We found that the mRNA expression of the following TRP channels: TRPV1 (p=0.0007), TRPV4 (p=0.0002), TRPM7 (p=0.0091) and TRPM8 (p=0.0008) are decreased and TRPC1 (p=0,02) are increased in SHR compared to control. In aortic rings preparations precontracted with 1 μM of phenylephrine, carvacrol (10-8 - 3x10-4 M) induced vasorelaxation in WKY (pD2 = 4.88  0.09, Emax = 100.73  2.24%, n = 6) and SHR (pD2 = 4.93  0.08, Emax= 110.06  2.07%, n = 6) in the presence of functional endothelium and that effect was not altered after endothelium removal in WKY (pD2 = 5.09  0.08, Emax = 99.60  0.88%, n = 6) and SHR (pD2 = 5.00  0.08, Emax = 101.23  1.96%, n = 6), proposing an endotheliumindependent mechanism. To assess the role of TRP channels, aortic rings were incubated with ruthenium red. In this assay, the vasorelaxant response was not changed in the WKY. On the other hand both potency (p<0.001) and efficacy (p<0.001) were reduced in SHR, suggesting that carvacrol could activate the subtypes TRPV in hypertensive animals. When using magnesium, equally potency (p<0.001) and pharmacological efficacy (p<0.01) were attenuated in both WKY and SHR, suggesting the involvement of TRPM7. In preparations with 2-APB, CPZ and BCTC, the vasorelaxant effect was potentiated (p<0.01) in both WKY and SHR, suggesting the participation of TRPV1, TRPM8 and TRPM7 channels in the vasorelaxant effect induced by carvacrol. Nevertheless, in the presence of capsaicin, the vasodilator effect was attenuated (p<0.001) in both WKY and SHR endorsing a possible action of carvacrol on TRPV1 and TRPV4 channel. In addition, in vivo studies showed that carvacrol produced hypotension and bradycardia in unanesthetized WKY and SHR. In order to address the cardiovascular responses in vivo, we performed experiments using ruthenium red and capsaicin to evaluate the contribution of TRP channels in this effect. Our results suggested an action of carvacrol on TRPV1 and TRPV4, confirming the in vitro assays. In conclusion, these results suggest that the expression of TRPV1, TRPV4, TRPM7 and TRPM8 was reduced and TRPC1 increased in SHR and carvacrol induced a vasorelaxant effect probably by acting on TRPV1, TRPV4, TRPC1, TRPM7 and TRPM8 in SHR. Furthermore, the in vivo effects induced by carvacrol exhibited a hypotensive and bradycardic activity and this effect, at least in part, is due to an activation of TRPV1 and TRPV4 channels in these responses.

Year

2022-12-06T23:47:14Z

Creators

Dantas, Bruna Priscilla Vasconcelos

Contribuição à quimiotaxonomia e ficoquímica de Canistrocarpus cervicornis (Kützing) De Paula & De Clerk (Dictyotaceae) : coletada nos litorais paraibano e fluminense

Marine biodiversity is presented as a promising source of new substances with high biological potential. Among the many organisms that inhabit the marine environment, we stress the brown algae belonging to the family Dictyotaceae. Genus Canistrocarpus was recently established, and some algae Dictyota formerly were reclassified as Canistrocarpus. This work describes the results of the phycochemical study of Canistrocarpus cervicornis (formerly classified as Dictyota pulchella), and the evaluation of the phycochemical profile of C. cervicornis collected on the coast of Paraíba and the coast of Rio de Janeiro, which contributed to the correct name of Dictyota pulchella as Canistrocarpus cervicornis. Alga samples were collected at Bessa Beach (coordinates 07º04'01 '' S and 34º49'35 '' W), city of João Pessoa (PB) in August 2010 and December 2012. The material was dried at room temperature and extraction was performed with CH2Cl2 : MeOH (2:1). The material was dried at room temperature in fresh air and extraction was performed with CH2Cl2: MeOH (2:1). The extract obtained the first collection was subjected to vacuum filtration on silica gel with solvent hexane, ethyl acetate and methanol in increasing gradient of polarity. The fraction hexane : ethyl acetate (85:15) was subjected to column chromatography yielded one new natural product, the dolastane diterpene 4,7-diacetoxy dichotenone A. The extract resulting from the second collection was divided in two parts. The first part was subjected to column chromatography resulted in the isolation of a known dolastane diterpene, 4-acetoxy-14-hidroxydolast-1(15),7,9-triene. A second aliquot of the extract was acetylated and subjected to column chromatography, which led to the isolation of three diterpenes, one dolastane unpublished in the literature, 4-desoxy-amijidictyol and two known secodolastanes, isolinearol acetate and indicol. Chemical constituents isolated were identified by spectroscopic methods 1H and 13C-1D and 2D NMR, optical rotation and comparison with literature data. This study contributed to the chemotaxonomy of the tribe Dictyoteae, especially C. cervicornis.

Year

2022-12-06T23:47:14Z

Creators

Figueiredo, Camila Silva de