RCAAP Repository

Aim Aridity gradients are of great interest for understanding the responses of biodiversity to water availability and water stress. However, little is known about the responses of many animal groups, which are crucial for assessing the effects of climate change. Here, we study the effects of aridity on dung beetle communities, a group with well-known responses to large-scale environmental gradients. Location Sahara, Kalahari and Chihuahuan deserts. Taxa Dung beetles of the family Scarabaeidae. Methods We conducted standardized surveys along approximately 400 km aridity gradients in each of the three deserts, and measured species richness, abundance, evenness and three aspects of trait-based functional diversity (functional richness, functional evenness and functional dispersion). By using randomization tests and linear mixed models, we compared observed with expected values for functional diversity indices from null models that hierarchically incorporate additional assembly constraints. Results Overall, we found a decrease of both taxonomic richness and functional dispersion along the three aridity gradients. Also, aridity seems to have mild effects on functional richness and functional evenness. Besides these general trends, we identified differences between deserts in the responses of both taxonomic and functional diversity. Main conclusions Aridity shows greater importance than competition and other processes of limiting similarity or stochastic processes in community assembly. Also, the functional hypervolume of dung beetle desert communities decreases with aridity not only due to species loss, but also because of selection of a few distinct phenotypes under harsh environmental conditions. Last, we observed that the different regional pools respond to aridity in different ways. Therefore, understanding future responses of dung beetle communities to the progressive decreases in water availability driven by climate change requires determining how the characteristics of the species in the regional pool interact with aridity-driven assembly processes.

Calls for transformations are clear and multiple pathways and alternative visions for the future have been defined. Yet, there is very little shared understanding of how such transformations come about and how knowledge-action gaps will be filled. This Special Feature focuses on how we can go beyond talking about transformation—the “blah blah blah”—and moving toward action for results. It does so by distinguishing between the means of transformation and the manner of transformation, two key dimensions to answering the question of “how.” The means can be understood as the many solutions, technical and practical methods, or actions that are presented as significant to transformative change. The manner, in contrast, represents the ways in which something is done, i.e., ways of acting. It describes the core values, principles, qualities, and relationships that not only underpin and motivate transformative change, but shape the process. Integrating rather than conflating the means and the manner is important to better understand how transformations come about. We then present insights from the collection of papers that focus on the “how” of transformation. The papers describe different ways of integrating the means and the manner in transformation processes. We have organized them thematically as follows: papers that draw on the integration of meaning making, the integration of learning and listening, and the integration of different ways of being and becoming. Drawing on both science and alternative ways of knowing, they weave together new narratives and stories about nature, society, and the future, inviting us to embark on the journey of creating sustainability pathways.

Elizabeth Bishop (1911-1979) starts exploring autobiographical material in her writing while living in Brazil, during the 1950s and 60s, as if diaspora enabled her to deal with issues of personal identity more openly. Focusing on the autobiographical short stories “In the Village” (1953) and “The Country Mouse” (1961), this essay looks at the representative strategies the writer chooses to portray the child protagonist’s body. Bishop’s traumatic childhood and her dislocation between borders and rural/urban landscapes (the Nova Scotia countryside and Boston) are inscribed in the protagonists’ bodily figurations, framed by a distanced narrator that highlights the tensions caused by the writer’s maternal and paternal families’ differentiated socialization practices. I will examine: i) the rhetorical strategies used by Bishop to exert formal control over her disturbing memories, namely through the reification of some of the characters’ bodies; ii) the deconstruction of the Great War victory narratives, with their strict gender distinctions. Considered the main index of personal identity in these fictional universes, the body will thus be read as a textual configuration that reflects the official discourses of citizenship in North America (Canada and the U.S.), and simultaneously resists these hegemonic proposals of identity, reclaiming its subjectivity.

Variable trace metal concentrations in the Precambrian ocean were closely linked to oxygen availability, although less is known about the drivers of seawater trace metal chemistry after the spread of complex life into the Phanerozoic eon. A major phytoplankton succession took place at the transition from the Palaeozoic to the Mesozoic era (~250 Myr ago), from an ocean dominated by the green Archaeplastida to secondary endosymbiotic algae with red-algal-derived plastids. Here, our comparative genomic analysis of 26 complete proteomes and metal domain analysis of additional 608 partially complete sequences of phytoplankton reveal that groups with different evolutionary history have distinct metal-binding proteins and contrasting metal acquisition strategies, adapted to differing availability of trace metals. The secondary-endosymbiont-bearing lineages are better adapted to well-oxygenated, nutrient-poor environments. This is supported by an enhanced thiol-based binding affinity of their transporters, coupled with minimized proteomic requirement for trace elements such as iron, copper and zinc at both protein and domain levels. Such different metal requirements across these lineages suggest a drastic decline in open-ocean trace metal concentrations at the inception of the Mesozoic, contributing to the shifts in phytoplankton communities that drove major changes in ocean chemical buffering.

External Thermal Insulation Composite Systems (ETICS) are multilayer solutions which provide an enhanced thermal performance to the building envelope. However, significant anomalies can be detected on ETICS facades, in some cases shortly after the application of these systems. This study intends to evaluate and compare the durability of six commercially available ETICS after two years of outdoor exposure at both urban and maritime conditions in Portugal. The systems were characterized by means of non-destructive testing (i.e., visual and microscopic assessment, water transport properties, thermal conductivity, surface roughness), thus allowing to evaluate the performance loss throughout natural aging. The bio-susceptibility and aesthetic properties (color and gloss) were also investigated. Results showed that the performance and durability of the complete system is significantly affected by the rendering system formulation. The lime-based specimens obtained the highest rate of mold development after one year of aging in a maritime environment, becoming considerably darker and with lower surface gloss. Fungal analysis of this darkish stained area indicated the presence of mold species of the genera Alternaria, Didymella, Cladosporium and Epicoccum, and yeasts of the genera Vishniacozyma and Cystobasidium. An increase of both capillary water absorption and water vapor permeability was also registered for the aged lime-based specimens. Acrylic-based systems obtained lower capillary water absorption after aging and greater dirt deposition on their surfaces, especially in urban conditions. These systems had also higher color variation and surface gloss decrease and slightly higher mold growth, when compared with those aged in a maritime environment. Finally, no mold growth was detected on the silicate-based specimens after two years of aging. However, these specimens obtained higher capillary water absorption and lower vapor permeability after aging, possibly leading to moisture accumulation within the system. Results contribute towards the development of ETICS with enhanced performance and durability.

Pears (Pyrus) are one of the most economically important fruits worldwide. The Pyrus genus is characterized by a high degree of genetic variability between species and interspecific hybrids, and several studies have been performed to assess this variability for both cultivated and wild accessions. These studies have mostly been limited by the resolving power of traditional molecular markers, although in the recent past the availability of reference genome sequences or SNP arrays for pear have enhanced the capability of high-resolution genomics studies. These tools can also be applied to better understand the intra-varietal (or clonal) variability in pear. Here we report the first high resolution genomics analysis of a pear clonal population using whole genome sequencing (WGS). Results showed unique signatures for the accumulation of mutations and transposable element insertions in each clone, which are likely related to their history of propagation and cultivation. The nucleotide diversity remained low in the clonal collection with the exception of few genomic windows, suggesting that balancing selection may be occurring. These windows included mainly genes related to plant fertility. Regions with higher mutational load were partially associated with transcription factors, probably reflecting the distinctive phenotypes in the collection. The annotation of variants also revealed the theoretical disruption of relevant genes in pear. Taken together, the results from this study show that pear clones accumulate mutations differently, and that those mutations can play a role on pear phenotypes, meaning that the study of pear clonal populations can be relevant in genetic studies, mainly when comparing with traditional association studies.

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We experimentally tested the effects of anticipated warmer temperatures on the feeding suc cess of invasive pumpkinseed and chameleon cichlid and the functionally similar Southern Iberian chub. Singly, prey captures by chub and the cichlid were constant but those by pumpkinseed were affected by warming. Moreover, at warmer temperatures, chub captured much less prey in the presence of the cichlid and prey faster in the presence of pumpkinseed than with conspecifics. Warming effects are likely species dependent, and asymmetries in feeding success between species may be enhanced at warmer temper atures to the benefit of warm-water invader

Carnivores tend to exhibit a lack of (or less pronounced) genetic structure at continental scales in both a geographic and temporal sense and this can confound the identification of post-glacial colonization patterns in this group. In this study we used genome-wide data (using genotyping by sequencing [GBS]) to reconstruct the phylogeographic history of a widespread carnivore, the red fox (Vulpes vulpes), by investigating broad-scale patterns of genomic variation, differentiation and admixture amongst contemporary populations in Europe. Using 15,003 single nucleotide polymorphisms (SNPs) from 524 individuals allowed us to identify the importance of refugial regions for the red fox in terms of endemism (e.g., Iberia). In addition, we tested multiple post-glacial recolonization scenarios of previously glaciated regions during the Last Glacial Maximum using an Approximate Bayesian Computation (ABC) approach that were unresolved from previous studies. This allowed us to identify the role of admixture from multiple source population post-Younger Dryas in the case of Scandinavia and ancient land-bridges in the colonization of the British Isles. A natural colonization of Ireland was deemed more likely than an ancient human-mediated introduction as has previously been proposed and potentially points to a larger mammalian community on the island in the early post-glacial period. Using genome-wide data has allowed us to tease apart broad-scale patterns of structure and diversity in a widespread carnivore in Europe that was not evident from using more limited marker sets and provides a foundation for next-generation phylogeographic studies in other non-model species.

Over the past few decades, species distribution modelling has been increasingly used to monitor invasive species. Studies herein propose to use Cellular Automata (CA), not only to model the distribution of a potentially invasive species but also to infer the potential of the method in risk prediction of Reticulitermes grassei infestation. The test area was mainland Portugal, for which an available presence-only dataset was used. This is a typical dataset type, resulting from either distribution studies or infestation reports. Subterranean termite urban distributions in Portugal from 1970 to 2001 were simulated, and the results were compared with known records from both 2001 (the publication date of the distribution models for R. grassei in Portugal) and 2020. The reported model was able to predict the widespread presence of R. grassei, showing its potential as a viable prediction tool for R. grassei infestation risk in wooden structures, providing the collection of appropriate variables. Such a robust simulation tool can prove to be highly valuable in the decision-making process concerning pest management.

The fitness of the endangered green sea turtle (Chelonia mydas) may be strongly affected by its gut microbiome, as microbes play important roles in host nutrition and health. This study aimed at establishing environmental microbial baselines that can be used to assess turtle health under altered future conditions. We characterized the microbiome associated with the gastrointestinal tract of green turtles from Guinea Bissau in different life stages and associated with their food items, using 16S rRNA metabarcoding. We found that the most abundant (% relative abundance) bacterial phyla across the gastrointestinal sections were Proteobacteria (68.1 ± 13.9% “amplicon sequence variants”, ASVs), Bacteroidetes (15.1 ± 10.1%) and Firmicutes (14.7 ± 21.7%). Additionally, we found the presence of two red algae bacterial indicator ASVs (the Alphaproteobacteria Brucella pinnipedialis with 75 ± 0% and a Gammaproteobacteria identified as methanotrophic endosymbiont of Bathymodiolus, with <1%) in cloacal compartments, along with six bacterial ASVs shared only between cloacal and local environmental red algae samples. We corroborate previous results demonstrating that green turtles fed on red algae (but, to a lower extent, also seagrass and brown algae), thus, acquiring microbial components that potentially aid them digest these food items. This study is a foundation for better understanding the microbial composition of sea turtle digestive tracts.

Adaptive radiation is a significant driver of biodiversity. Primarily studied in animal systems, mechanisms that trigger adaptive radiations remain poorly understood in plants. A frequently claimed indicator of adaptive radiation in plants is growth form diversity when tied to the occupation of different habitats. However, it remains obscure whether morphological adaptations manifest as growth form diversity per se or as its constituent traits. We use the classic Aeonium radiation from the Canary Islands to ask whether adaptation across climatic space is structured by growth form evolution. Using morphological sampling with site-associated climate in a phylogenetic context, we find that growth forms dictate adaptations to the local environment. Furthermore, we demonstrate that the response of specific traits to analogous environments is antagonistic when growth forms are different. This finding suggests for the first time that growth forms represent particular ecological functions, allowing the co-occurrence of closely related species, being a product of divergent selection during evolution in sympatry.

The effects of metals on plants and herbivores, as well as the interaction among the latter, are well documented. However, the effects of simultaneous herbivory and metal accumulation remain poorly studied. Here, we shed light on this topic by infesting cadmium-accumulating tomato plants (Solanum lycopersicum), either exposed to cadmium or not, with herbivorous spider mites, Tetranychus urticae or T. evansi during 14 days. Whereas on plants without cadmium T. evansi had higher growth rate than T. urticae, on plants with cadmium both mite species had similar growth rates, which were lower than on plants without metal. Plants were affected by both cadmium toxicity and by herbivory, as shown by leaf reflectance, but not on the same wavelengths. Moreover, changes in leaf reflectance on the wavelength affected by herbivores were similar on plants with and without cadmium, and vice versa. Long-term effects of cadmium and herbivory did not affect H2O2 concentrations in the plant. Finally, plants infested with spider mites did not accumulate more cadmium, suggesting that metal accumulation is not induced by herbivory. We thus conclude that cadmium accumulation affects two congeneric herbivore species differently and that the effects of herbivory and cadmium toxicity on plants may be disentangled, via leaf reflectance, even during simultaneous exposure.

Este ensaio pretende oferecer uma visão do percurso de consolidação identitária da Comédia de Costumes Britânica desde o seu advento, no período da Restauração (segunda metade do séc. XVII), até à segunda metade do séc. XX, através da interpelação de comédias consideradas paradigmáticas face ao contexto epocal em que se inscrevem. O corpus em análise inclui as seguintes comédias: The Way of the World (1700) de William Congreve, The School for Scandal (1777) de Richard Sheridan, An Ideal Husband (1895) de Oscar Wilde, Pygmalion (1914) de George Bernard Shaw, Hay Fever (1925) de Nöel Coward e Absurd Person Singular (1972) de Alan Ayckbourn. A análise proposta debruça-se, fundamentalmente, sobre os seguintes tópicos: 1) A plutocracia e a meritocracia: um diálogo. 2) Simulações e dissimulações, ou os jogos da mentira e da verdade. 3) O respeito/desrespeito pelo Outro: que Outro? A fim de debater tais questões dentro de uma perspectiva de Crítica Ética, é convocado o discurso filosófico e sociológico dos seguintes autores: Aristóteles, Immanuel Kant, Friedrich Nietzsche, Henri Bergson, Jean Baudrillard, Emmanuel Levinas, Zygmunt Bauman e Gilles Lipovetsky.

Predation risk profoundly shapes how animals behave and is one of the main forces driving the formation or maintenance of groups. For some species, group living may be facultative, and individuals may live solitarily or aggregate with conspecifics or heterospecifics, but the advantages of each strategy are still poorly known. Here, we investigated whether a predominantly solitary breeding species, the European roller, Coracias garrulus, acquires antipredator benefits from nesting in mixed-species colonies dominated by lesser kestrels, Falco naumanni. We compared the risk-taking behaviour of solitary rollers and rollers breeding in colonies by conducting two experiments. First, we investigated rollers’ latency to resume incubation when presented with a novel object, and second assessed their latency to resume chick provisioning and their investment in mobbing behaviour towards a predator model. We additionally compared the breeding performance and nest predation rate of rollers in each social context (solitary versus colonial) using data from 300 breeding attempts across 6 years. We found that rollers breeding in colonies returned to their nests sooner during the presentation of both the novel object and the predator model and attacked the predator model less frequently than solitary rollers, suggesting they can use heterospecifics as cues in deciding whether it is safe to return to their nests. In addition, rollers in colonies suffered less nest predation than solitary ones, but this did not translate into a higher productivity. Future studies should investigate whether breeding in colonies provides other advantages to rollers, such as increased adult survival or fitness.

Although successful at recovering endangered populations, conservation actions based on nest provisioning seldom consider how they shape the composition of communities and alter interspecific interactions. Specifically, the extent to which dietary overlap within these communities may affect the conservation of target species has rarely been assessed. In Southern Europe, sites of large-scale nest-site provisioning aimed at recovering Lesser Kestrels Falco naumanni populations have attracted non-target bird species, resulting in mixed breeding assemblages that might promote interspecific competition for resources during breeding. Here we used stable isotope analysis (δ15N and δ13C) to assess inter- and intraspecific dietary segregation in these assemblages and investigate the mechanisms allowing species coexistence. We examined resource partitioning and trophic niche overlap among Lesser Kestrels, Common Kestrels Falco tinnunculus, European Rollers Coracias garrulus, Western Barn Owls Tyto alba, Little Owls Athene noctua and Spotless Starlings Sturnus unicolor; and within species between parents and their offspring. Similar isotope ratios and highly overlapped niches, particularly among Lesser Kestrels, Rollers and Starlings, suggest limited dietary segregation and use of similar prey. Within species, parent–offspring segregation was marked across all species. Our results indicate that species breeding in these assemblages occupy similar ecological niches, despite a potential increase in competition. High resource availability in the area may permit coexistence but the viability of mixed-species groups may be compromised in areas with limited resources, which are predicted to expand with ongoing human- and climate-induced changes. Conservation practices based on nest provisioning should consider the ecological niches of target and non-target species as well as their interactions.

Cystic echinococcosis (CE) and alveolar echinococcosis (AE) are among the most relevant zoonoses in Europe. According to the European Food Safety Authority (EFSA), Echinococcus granulosus sensu lato causing CE is the most significant foodborne parasite in South-Western Europe, followed by Echinococcus multilocularis, the etiological agent of AE. Among the challenges and opportunities highlighted in the literature to combat these diseases are the need to evaluate and increase awareness of stakeholders. In Portugal, Municipal Veterinary Practitioners (MVP) are the animal health authority at the municipality level, playing a crucial role in diagnosis, prevention and control of infectious diseases in animals, helping to mitigate transmission to humans. However, their knowledge, attitudes, and practices (KAP) towards CE, as well as awareness of AE expansion across Europe, remain overlooked. In this work, a cross-sectional study was conducted for the first time in Portugal to bridge knowledge gaps concerning CE and AE, using an online self-administered questionnaire collecting information on the municipal kennel, KAP towards CE, and understanding of AE epidemiology. Eighty-three MVP from mainland and islands completed the questionnaire, with highest representability from the central-northern region. MVP had, on average, a medium to high level of knowledge of CE but acquaintance with AE was insufficient, although echinococcosis was frequently mentioned as target of health education sessions (77.0%). A high rate (60%) of reported entries into municipal kennels of stray dogs originating from countries with AE endemic areas was registered, suggesting that the presence of these potentially AE-infected stray dogs pose public health risks. Most kennels did not perform routine coprological analysis or faecal matter disinfection after dog internal deworming. The lack of proper training and well-conceived written plans of infection control and prevention were evidenced in several kennels. Altogether, our findings highlight the need to update knowledge and practice of MVP under the One Health approach, through reinforced education, training and communication involving all stakeholders.

The mcr-1 gene spread is worldwide recognized as a public health threat at multidrug-resistant infections therapy level. Here, we report for the first time, to the best of our knowledge, the detection of the globally distributed IncX4 plasmid carrying mcr-1 (mcr-1/IncX4) in Escherichia coli isolated from a wild mammal in Portugal and Europe. This plasmid was found in a ST533 E. coli isolate with a multidrug-resistant profile, virulence potential, and possibly phylogenetically related to human isolates. Our work contributes to highlight the importance of antimicrobial resistance (AMR) surveillance in wildlife, an important compartment of the whole ecosystem often overlooked in the fight against AMR. IMPORTANCE Colistin resistance mediated by plasmids is recognized worldwide as an emergency problem connected with the whole ecosystem, since is well described in the interface of the human-animal-environment. The plasmid IncX4 is reported as one of the most prevalent plasmids harboring the gene mcr-1. On an European scale the plasmid IncX4 carrying mcr-1 has been described in humans, the environment, and animals, including wildlife, but only in wild birds. This study shows the first report of the plasmid IncX4 harboring mcr-1 in a wild mammal in Portugal and Europe, identified in a ST533 E. coli commensal that is, curiously, more related to isolates from humans than from livestock. Our findings show that the plasmid IncX4 harboring mcr-1 is well established in a colistin resistance drive embracing the whole ecosystem.

Objetivo: O objetivo deste estudo foi avaliar as forças retentivas ao longo do tempo de ganchos de PPRs produzidos pelo método digital. Materiais e métodos: Três dentes pré-molares foram talhados com sede de apoio oclusal mesial e incluídos em blocos de acrílico (9x20x40mm). Estes foram digitalizados com digitalizador laboratorial (Zirkonzahn Scanner S600). Após análise e determinação do eixo de inserção, foram desenhados, no programa Partial Planner Zirkonzahn, dois ganchos com apoio oclusal mesial por dente: um gancho do tipo ação reversa e um do tipo retenção posterior. O ficheiro foi enviado para produção de 6 ganchos metálicos em liga SP2 de Co-Cr no equipamento EOSINT M270 pelo método de sinterização direta de metal a laser. Utilizando uma máquina de testes universal Instron 5544 foram realizados 20000 ciclos de inserção e desinserção, dos ganchos no respetivo dente com célula de carga de 100 N e velocidade de 2,5 mm/seg. Foi registada a força retentiva em newtons a cada 1000 ciclos e calculada a variação da retenção ao longo do tempo. A análise estatística obedeceu ao teste não paramétrico de Mann-Whitney e ao nível de significância de 5%. Resultados: Verificou-se um máximo de variação de força de 3,74 N aos 16000 ciclos, no gancho de retenção posterior e mínimo de -24,28 N aos 1000 ciclos, no gancho de ação reversa. Os ganchos de ação reversa apresentaram uma variação de retenção menos significativa quando comparados com os ganchos de retenção posterior aos 4000 e 5000 ciclos. Nos restantes ciclos não se observaram diferenças. Conclusão: Durante os 20000 ciclos verificou-se uma variação de retenção reduzida, independentemente do tipo de gancho. Não houve diferença de variação da força de retenção entre os dois tipos de gancho, na maioria dos ciclos.

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