RCAAP Repository

A Perturbação do Espetro do Autismo (PEA) é uma perturbação do neurodesenvolvimento caracterizada por défices na comunicação e interação social e pela presença de padrões repetitivos de comportamentos, interesses ou atividades. Atinge uma prevalência que ronda o 1% da população, efetuando-se o diagnóstico entre os 3 e os 4 anos. O quadro marca-se por uma acentuada heterogeneidade clínica, com possibilidade de défices na linguagem, na cognição, entre outros. A abordagem terapêutica assenta num plano individualizado, que pode incluir terapêuticas farmacológicas e não farmacológicas, tais como terapia da fala, intervenção comportamental, ensino estruturado, intervenções familiares, entre outras. Com este trabalho procurámos avaliar, após 2 anos, a evolução destas crianças a nível dos sintomas da PEA e do seu desenvolvimento psicomotor. Procurámos ainda relacionar esta evolução com as intervenções terapêuticas realizadas. Nesse sentido desenhamos um estudo retrospetivo longitudinal com recolha de dados qualitativos (diário clínico) e dados quantitativos (instrumentos de avaliação do neurodesenvolvimento), sendo a análise estatística feita através do SPSS Statistics 25. Constatámos que, na nossa amostra, o diagnóstico é feito mais precocemente do que o descrito na literatura, sendo a perturbação da linguagem a comorbilidade mais prevalente. Consequentemente, foi nela que se efetuou um maior investimento terapêutico, obtendo-se resultados mais expressivos e estatisticamente significativos de melhoria, correlacionando-se a terapia da fala com a mesma. Observámos também melhorias significativas a nível do quociente geral do desenvolvimento psicomotor bem como da autonomia pessoal. No que respeita à sintomatologia de autismo observou-se uma melhoria qualitativa. Concluímos que a evolução na nossa amostra foi significativa ao nível das comorbilidades do autismo, em específico na área da linguagem, e que a terapia que mais contribuiu foi a terapia da fala. Contudo, considera-se que a intensidade terapêutica é muito reduzida face ao aconselhado internacionalmente. Estes e outros achados irão contribuir para a prática clínica em neurodesenvolvimento.

Humanity in the modern world is confronted with diverse problems at several levels. The environmental concern is probably the most important as it threatens different ecosystems, food, and farming as well as humans, animals, and plants. More specifically, salinization of agricultural soils is a global concern because of on one side, the permanent increase of the areas affected, and on the other side, the disastrous damage caused to various plants affecting hugely crop productivity and yields. Currently, great attention is directed towards the use of Plant Growth Promoting Bacteria (PGPB). This alternative method, which is healthy, safe, and ecological, seems to be very promising in terms of simultaneous salinity alleviation and improving crop productivity. This review attempts to deal with different aspects of the current advances concerning the use of PGPBs for saline stress alleviation. The objective is to explain, discuss, and present the current progress in this area of research. We firstly discuss the implication of PGPB on soil desalinization. We present the impacts of salinity on crops. We look for the different salinity origin and its impacts on plants. We discuss the impacts of salinity on soil. Then, we review various recent progress of hemophilic PGPB for sustainable agriculture. We categorize the mechanisms of PGPB toward salinity tolerance. We discuss the use of PGPB inoculants under salinity that can reduce chemical fertilization. Finally, we present some possible directions for future investigation. It seems that PGPBs use for saline stress alleviation gain more importance, investigations, and applications. Regarding the complexity of the mechanisms implicated in this domain, various aspects remain to be elucidated.

Introdução: A patologia alérgica, como a asma, a rinite e a alergia alimentar, é uma condição crónica e muito prevalente na população em geral e, em especial, na idade pediátrica, com potencial impacto negativo na qualidade de vida. Com a apresentação de um caso clínico pretende-se compreender melhor a patologia alérgica e como esta afeta várias áreas da vida dos indivíduos e da respetiva família. Caso Clínico: Criança do género masculino com 8 anos, seguida em consulta de imunoalergologia por asma grave sob terapêutica mensal com omalizumab desde setembro de 2017, rinite alérgica e alergia alimentar múltipla (leite e ovo) com episódios de anafilaxia grave. Discussão: A marcha alérgica inicia-se na maioria dos casos na idade pediátrica, com alergia alimentar e/ou dermatite atópica, seguindo-se a asma e a rinite alérgica, especialmente quando existe história familiar de atopia, apesar de ter uma componente ambiental como fator de risco ou de proteção. Asma e anafilaxia são duas das manifestações alérgicas mais graves e cada uma delas pode influenciar a outra em termos da gravidade dos sintomas e respetivo tratamento. Frequentemente, os sintomas de anafilaxia acontecem na presença de cuidadores, os quais nem sempre estão conscientes dos sinais e sintomas e podem ser relutantes no uso de adrenalina, apesar de esta ser a primeira linha terapêutica. Conclusão: Apesar de muito comum e prevalente, a anafilaxia continua a ser subdiagnosticada e subtratada e tem como principal causa a alergia alimentar, sendo de pior prognóstico na presença de outras doenças alérgicas, em especial asma grave, de difícil controlo. Quem vive com esta condição geralmente tem menor qualidade de vida, necessitando de muitos cuidados a vários níveis para se manter sem episódios anafiláticos, condicionando a vida familiar e social, embora, apesar de evicção alimentar estrita, exista possibilidade de reações alérgicas graves, por alergénios ocultos ou contaminações.

Novel outbreaks of emerging pathogens require rapid responses to enable successful mitigation. We simulated a 1‐day emergency meeting where experts were engaged to recommend mitigation strategies for a new outbreak of the amphibian fungal pathogen Batrachochytrium salamandrivorans. Despite the inevitable uncertainty, experts suggested and discussed several possible strategies. However, their recommendations were undermined by imperfect initial definitions of the objectives and scope of management. This problem is likely to arise in most real‐world emergency situations. The exercise thus highlighted the importance of clearly defining the context, objectives, and spatial–temporal scale of mitigation decisions. Managers are commonly under pressure to act immediately. However, an iterative process in which experts and managers cooperate to clarify objectives and uncertainties, while collecting more information and devising mitigation strategies, may be slightly more time consuming but ultimately lead to better outcomes.

Background: Patients in the late stages of parkinsonism are highly dependent on others in their self-care and activities of daily living. However, few studies have assessed the physical, psychological and social consequences of caring for a person with late-stage parkinsonism. Patients and methods: Five hundred and six patients and their caregivers from the Care of Late Stage Parkinsonism (CLaSP) study were included. Patients’ motor and non-motor symptoms were assessed using the UPDRS and Non-motor symptom scale (NMSS), Neuropsychiatric inventory (NPI-12), and caregivers’ health status using the EQ-5D-3 L. Caregiver burden was assessed by the Zarit Burden Interview (ZBI). Results: The majority of caregivers were the spouse or life partner (71.2%), and were living with the patient at home (67%). Approximately half of caregivers reported anxiety/depression and pain/discomfort (45% and 59% respectively). The factors most strongly associated with caregiver burden were patients’ neuropsychiatric features on the total NPI score (r = 0.38, p < 0.0001), total NMSS score (r = 0.28, p < 0.0001), caring for male patients and patients living at home. Being the spouse, the hours per day assisting and supervising the patient as well as caregivers’ EQ-5D mood and pain scores were also associated with higher ZBI scores (all p < 0.001). Conclusion: The care of patients with late stage parkinsonism is associated with significant caregiver burden, particularly when patients manifest many neuropsychiatric and non-motor features and when caring for a male patient at home.

Neste artigo recorre-se à análise qualitativa do enredo de histórias de ficção científica sobre o trabalho de um grupo de cientistas, redigidas por três alunos portugueses da disciplina de Ciências da Terra e da Vida do 11º ano, e à realização de entrevistas semi-estruturadas, com o objectivo duplo de diagnosticar concepções sobre os cientistas e discutir as potencialidades desta metodologia na investigação e na educação em ciência. A metodologia utilizada revelou-se bastante adequada ao diagnóstico de concepções dos alunos sobre as características dos cientistas, a actividade científica e as interacções entre ciência, tecnologia e sociedade. Entre os participantes, foi notória a falta de conhecimentos processuais e epistemológicos sobre a ciência, bem como a existência de diversas concepções estereotipadas e deturpadas sobre as características e a actividade dos cientistas. Diversos factores parecem contribuir para esta situação, nomeadamente, as práticas de sala de aula utilizadas pelos seus professores, as imagens de ciência veiculadas pelos meios de comunicação social e a ausência, nas aulas de ciências, de qualquer tipo de reflexão crítica sobre essas imagens.

A key strategy in the European Union’s ambition to establish an ‘Energy Union’ that is not just clean, but also fair, consists of empowering citizens to actively interact with the energy market as self-consumers or prosumers. Although renewable energy sources (RES) prosumerism has been growing for at least a decade, two new EU directives are intended to legitimise and facilitate its expansion. However, little is known about the full range of prosumers against which to measure policy effectiveness. We carried out a documentary study and an online survey in nine EU countries to shed light on the demographics, use of technology, organisation, financing, and motivation as well as perceived hindering and facilitating factors for collective prosumers. We identified several internal and external obstacles to the successful mainstreaming of RES prosumerism, among them a mismatch of policies with the needs of different RES prosumer types, potential organisational weaknesses as well as slow progress in essential reforms such as decentralising energy infrastructures. Our baseline results offer recommendations for the transposition of EU directives into national legislations and suggest avenues for future research in the fields of social, governance, policy, technology, and business models.

Functional movement disorders (FMD) are proposed to reflect a specific problem with voluntary control of movement, despite normal intent to move and an intact neural capacity for movement. In many cases, a positive diagnosis of FMD can be established on clinical grounds. However, the diagnosis remains challenging in certain scenarios, and there is a need for predictors of treatment response and long-term prognosis.In this context, we performed a systematic review of biomarkers in FMD. Eighty-six studies met our predefined criteria and were included.We found fairly reliable electroencephalography and electromyography-based diagnostic biomarkers for functional myoclonus and tremor. Promising biomarkers have also been described for functional paresis, gait and balance disorders. In contrast, there is still a lack of diagnostic biomarkers of functional dystonia and tics, where clinical diagnosis is often also more challenging. Importantly, many promising findings focus on pathophysiology and reflect group-level comparisons, but cannot differentiate on an individual basis. Some biomarkers also require access to time-consuming and resource-consuming techniques such as functional MRI.In conclusion, there are important gaps in diagnostic biomarkers in FMD in the areas of most clinical uncertainty. There is also is a lack of treatment response and prognostic biomarkers to aid in the selection of patients who would benefit from rehabilitation and other forms of treatment.

Sea urchins have hundreds of specialized adhesive organs, the tube feet, which play a key role in locomotion, substrate attachment and food capture. Tube feet are composed by two functional units: a proximal cylindrical stem that is mobile and flexible, attached to a distal flattened disc that produces adhesive secretions. Oral tube feet discs possess a specialized duo-glandular epidermis that produces adhesive and de-adhesive secretions, enabling strong but reversible adhesion to the substrate. Due to the growing interest in biomimetic adhesives, several studies have been carried out to characterize sea urchin adhesives, and up to date, it has been shown that it is composed by proteins and glycans. The protein fraction has been the subject of several studies, that pin-pointed several adhesion-related candidates. Contrastingly, little is known about the glycans that compose sea urchin adhesives. This study aims at contributing to this topic by focusing on the characterization of the glycosidic fraction of the adhesive secreted by the sea urchin Paracentrotus lividus (Lamarck, 1816), using a battery of 22 lectins, applied to 3 complementary techniques. Our results show that five lectins label exclusively the disc adhesive epidermis and simultaneously the secreted adhesive, being, therefore, most likely relevant for sea urchin adhesion. In addition, it was possible to determine that the glycosidic fraction of the adhesive is composed by a high molecular weight glycoprotein containing N-acetylglucosamine oligomers.

Introduction: Inflammation contributes to the initiation and progression of atherosclerosis, although the underlying inflammatory pathways are not entirely known. Specifically, the role of the proinflammatory soluble CD40 ligand (sCD40L) on the expression of chronic coronary syndrome (CCS) is not completely understood. We evaluated whether sCD40L expression is associated with the presence of CCS and with the clinical and anatomical severity of CCS. Methods: We prospectively recruited 94 participants, assigned to two groups matched by age and sex, without coronary artery disease (n=26) and with CCS (n=68). Clinical, laboratory and anatomical data were prospectively collected, and serum levels of sCD40L were measured. Results: In patients with CCS, classic cardiovascular risk factors were more prevalent, and the sCD40L levels, leukocyte and neutrophil counts, and neutrophil/lymphocyte ratio, but not the C-reactive protein levels, were significantly higher than those in controls. sCD40L was independently associated with the presence of obstructive coronary artery disease in multivariate analysis. Regarding CCS severity, sCD40L levels showed a significant stepwise increase with increasing angina severity (ANOVA P=0.001). In addition, sCD40L was independently associated with the anatomical severity of coronary artery disease, as assessed by the Gensini score. Among patients with CCS, those with previous coronary artery bypass grafting (n=23) had lower sCD40L levels than patients waiting for revascularization (n=45) [4.3 (2.1) ng/mL vs. 6.8 (3.5) ng/mL, P=0.001]. Conclusions: The expression of the proinflammatory sCD40L was associated with the presence of CCS and reflected the clinical and anatomical severity of CCS. In addition, we describe for the first time the association between prior CABG and reduced sCD40L levels in patients with CCS.

The unsustainable wildmeat trade has been long recognized as a threat to wildlife. Yet, its impact on marine species has been underresearched. Sea turtles have been historically threatened by unsustainable trade, but there are few studies of consumer profile, motivations, and preferences. We conducted a survey in São Tomé Island, Gulf of Guinea, West Africa, including eight rural communities (n = 1,160) and the capital city of São Tomé (n = 628). We estimated prevalence of consumption, preference and availability of sea turtle meat and eggs. About 25% of rural and 32% of urban respondents had consumed meat in the past year. Around 25% of rural respondents were egg consumers, whereas little consumption was found in urban areas. Social norms were a predictor of consumption of sea turtle meat and eggs in rural communities but not in São Tomé city. Regarding influencers, teachers and religious leaders were the most trusted sources in rural communities, while teachers and NGOs were most trusted in São Tomé city. Radio and television were the most trusted channels in rural communities, while urban dwellers trusted radio the most. This research showcases how a structured approach to audience research can obtain behavioral insights that can guide behavior change efforts.

γδ T cells are a unique T cell subpopulation that are rare in secondary lymphoid organs but enriched in many peripheral tissues, such as the skin, intestines and lungs. By rapidly producing large amounts of cytokines, γδ T cells make key contributions to immune responses in these tissues. In addition to their immune surveillance activities, recent reports have unravelled exciting new roles for γδ T cells in steady-state tissue physiology, with functions ranging from the regulation of thermogenesis in adipose tissue to the control of neuronal synaptic plasticity in the central nervous system. Here, we review the roles of γδ T cells in tissue homeostasis and in surveillance of infection, aiming to illustrate their major impact on tissue integrity, tissue repair and immune protection.

Preclinical trials are essential to test efficacious options to substitute the temporomandibular joint (TMJ) disk. The contemporary absence of an ideal treatment for patients with severe TMJ disorders can be related to difficulties concerning the appropriate study design to conduct preclinical trials in the TMJ field. These difficulties can be associated with the use of heterogeneous animal models, the use of the contralateral TMJ as control, the absence of rigorous randomized controlled preclinical trials with blinded outcomes assessors, and difficulties involving multidisciplinary teams.

Os acidentes de viação são uma das principais causas de morbimortalidade a nível mundial. O crânio é a região anatómica mais frequentemente afetada e as fraturas são uma das consequências mais comuns. Logo, os acidentes de viação são uma das causas mais frequentes de fratura do osso Temporal. O diagnóstico de fratura do osso temporal faz-se através de um exame objetivo minucioso e através de exames de imagem. As fraturas podem ser transversais ou longitudinais. Noutro sistema de classificação, podem ser optic capsule sparing ou optic capsule violating. A hipoacúsia é uma das consequências mais frequentes da fratura do osso Temporal. Muitas outras complicações desta fratura podem ocorrer. A hipoacúsia pode afetar bastante a qualidade de vida dos indivíduos. A prevenção dos acidentes de viação é uma das medidas preventivas de fratura do osso Temporal.

The temporomandibular joint interposal study (TEMPOJIMS) is a rigorous preclinical trial divided in 2 phases. In phase 1 the authors investigated the role of the TMJ disc and in phase 2 the authors evaluated 3 different interposal materials. The present work of TEMPOJIMS - phase 1, investigated the effects of bilateral discectomy and discopexy in sheep mastication and rumination.

Caffeine, a stimulant largely consumed around the world, is a non-selective adenosine receptor antagonist, and therefore caffeine actions at synapses usually, but not always, mirror those of adenosine. Importantly, different adenosine receptors with opposing regulatory actions co-exist at synapses. Through both inhibitory and excitatory high-affinity receptors (A1R and A2R, respectively), adenosine affects NMDA receptor (NMDAR) function at the hippocampus, but surprisingly, there is a lack of knowledge on the effects of caffeine upon this ionotropic glutamatergic receptor deeply involved in both positive (plasticity) and negative (excitotoxicity) synaptic actions. We thus aimed to elucidate the effects of caffeine upon NMDAR-mediated excitatory post-synaptic currents (NMDAR-EPSCs), and its implications upon neuronal Ca2+ homeostasis. We found that caffeine (30-200 μM) facilitates NMDAR-EPSCs on pyramidal CA1 neurons from Balbc/ByJ male mice, an action mimicked, as well as occluded, by 1,3-dipropyl-cyclopentylxantine (DPCPX, 50 nM), thus likely mediated by blockade of inhibitory A1Rs. This action of caffeine cannot be attributed to a pre-synaptic facilitation of transmission because caffeine even increased paired-pulse facilitation of NMDA-EPSCs, indicative of an inhibition of neurotransmitter release. Adenosine A2ARs are involved in this likely pre-synaptic action since the effect of caffeine was mimicked by the A2AR antagonist, SCH58261 (50 nM). Furthermore, caffeine increased the frequency of Ca2+ transients in neuronal cell culture, an action mimicked by the A1R antagonist, DPCPX, and prevented by NMDAR blockade with AP5 (50 μM). Altogether, these results show for the first time an influence of caffeine on NMDA receptor activity at the hippocampus, with impact in neuronal Ca2+ homeostasis.

In this article the authors resort to a qualitative analysis of the plot of science fiction stories about a group of scientists, written by two 11th-grade Earth and Life Science students (aged 17), and to semi-structured interviews, with the double purpose of diagnosing their conceptions of the nature of science (namely, as regards scientists’ activity), and discussing the potentialities of this methodology in terms of research and education in science. The adopted methodology proved particularly effective in diagnosing the students’ conceptions of scientists’ characteristics, scientific activity, and science–technology–society interactions. The limited content of certain conceptions and a certain lack of knowledge on the part of the students concerning the processes and the epistemology of science highlight the need to pay explicit attention in science classes to the nature of scientific activity. Some of the ideas brought up by the students clearly show the influence of stereotypes and catastrophic scenarios depicted in films, television programs, and books, revealing media’s limitations when divulging scientific and technological themes to the general public and stressing the need for the school to promote a critical debate about science and technology images conveyed by the media.

This Communication reports the first general method for rapid, chemoselective, and modular functionalization of serine residues in native polypeptides, which uses a reagent platform based on the P(V) oxidation state. This redox-economical approach can be used to append nearly any kind of cargo onto serine, generating a stable, benign, and hydrophilic phosphorothioate linkage. The method tolerates all other known nucleophilic functional groups of naturally occurring proteinogenic amino acids. A variety of applications can be envisaged by this expansion of the toolbox of site-selective bioconjugation methods.

LINC complexes are transmembrane protein assemblies that physically connect the nucleoskeleton and cytoskeleton through the nuclear envelope. Dysfunctions of LINC complexes are associated with pathologies such as cancer and muscular disorders. The mechanical roles of LINC complexes are poorly understood. To address this, we used genetically encoded FRET biosensors of molecular tension in a nesprin protein of the LINC complex of fibroblastic and epithelial cells in culture. We exposed cells to mechanical, genetic, and pharmacological perturbations, mimicking a range of physiological and pathological situations. We show that nesprin experiences tension generated by the cytoskeleton and acts as a mechanical sensor of cell packing. Moreover, nesprin discriminates between inductions of partial and complete epithelial-mesenchymal transitions. We identify the implicated mechanisms, which involve α-catenin capture at the nuclear envelope by nesprin upon its relaxation, thereby regulating β-catenin transcription. Our data thus implicate LINC complex proteins as mechanotransducers that fine-tune β-catenin signaling in a manner dependent on the epithelial-mesenchymal transition program.

Temporomandibular Joint (TMJ) arthroscopy is considered an effective and safe minimally invasive surgical approach. While the long-term outcomes of arthroscopy tend to be positive and free of secondary effects, patients occasionally complain about their hearing following the treatment. The aim of this prospective study was to investigate possible hearing changes associated with TMJ arthroscopy. Pure-tone audiograms were performed in patients two weeks before TMJ arthroscopy and repeated six weeks after intervention. A total of 15 patients (mean age of 41.73±16.36) were enrolled; 25 TMJ arthroscopies were performed (five unilateral and ten bilateral). Statistically significant differences were found between preoperative and postoperative audiograms in the frequencies 256Hz (P=0.011) and 8kHz (P=0.058, borderline). For the frequency 256Hz the difference was favourable, but not superior to 5dB. For the frequency 8kHz, in three patients the TMJ arthroscopy resulted in a decrease of 10dB. However, no clinical hearing changes or complaints were observed in the involved patients. No differences in audiograms between level 1 or 2 arthroscopy were observed. The study reinforces the safety of the TMJ arthroscopy level 1 and 2 with the reported protocol. The authors recommend larger studies to validate the results, specially for frequency 8kHz.