Repositório RCAAP

Resumo O presente trabalho é parte integrante do projeto Flora do Espírito Santo e teve como objetivo caracterizar morfologicamente as espécies de Amaryllidaceae ocorrentes no estado, bem como fornecer dados sobre sua distribuição geográfica e comentários sobre a biologia e ambiente das espécies. Para isto, expedições de coleta foram realizadas no período de outubro/2016 a dezembro/2017 buscando inventariar as espécies da família ocorrentes no estado. Três gêneros e 17 espécies foram registradas no Espírito Santo. Hippeastrum é o gênero com maior riqueza de espécies (8 spp.), seguido de Griffinia (7 spp.) e Crinum (2 spp.). Griffinia colatinensis possui status de conservação definido como “Criticamente em Perigo” (CR), enquanto G. espiritensis, G. liboniana, H. brasilianum, H. reginae e H. striatum como “Em Perigo” (EN). Caracteres como escapo floral (sólido ou fistuloso), número e grau de fusão das brácteas da inflorescência, comprimento do tubo do hipanto, padrão de coloração, corona (presença/ausência e tipos), tipo de ambiente e bulbo subterrâneo ou exposto, foram considerados importantes para distinção taxonômica do grupo analisado. Neste estudo são fornecidas descrições taxonômicas, chaves de identificação para os gêneros e as espécies, mapas de distribuição, ilustrações e pranchas fotográficas.

Resumo A deiscência não simultânea dos frutos de bromélias deve ser considerada para fins de propagação por sementes. O objetivo deste estudo foi avaliar o melhor método para produção de plantas da bromélia imperial considerando a colheita simultânea das sementes de diferentes posições do escapo floral no período da deiscência dos frutos da base e seu armazenamento para posterior uso na obtenção de plantas. As sementes foram colhidas da base, meio e topo do escapo floral e armazenadas por 26, 61, 154 e 213 dias a 10 °C e a 25 °C. Foram avaliadas a emergência de plantas em gerbox com papel de filtro e a germinação e crescimento in vitro com 10, 20 e 30 g.L-1 de sacarose. As sementes colhidas da base e armazenadas por até dois meses a 10 °C foram as que apresentaram maior porcentagem de emergência de plantas (57%) e a melhor concentração de sacarose para germinação in vitro foi de 10 g.L-1 e para o crescimento, 20 g.L-1. Considerando-se a vulnerabilidade desta bromélia, o aproveitamento, ao mesmo tempo, das sementes de todo o escapo floral pode ser uma estratégia de conservação.

Resumo A pesquisa foi desenvolvida com um grupo Kaiowá, do tronco linguístico Tupi-Guarani. O território Kaiowá é denominado Tekoha. Esse grupo vem ocupando uma fazenda cujo território, embora tenha sido reconhecido como a terra original do Tekoha Taquara em 2010 pela Fundação Nacional do Índio, teve a demarcação suspensa por liminar. A ocupação é considerada ilegal e, os indígenas vêm sendo dizimados. As lideranças Kaiowá nos desafiaram a mostrar que o conhecimento que detêm sobre a flora local, seria uma evidência que legitima a reivindicação da área. Para atender a reivindicação o trabalho foi alicerçado sob hipóteses: recursos iguais estão disponíveis aos indígenas e não indígenas e são valorizados distintamente; os Kaiowá possuem conhecimento exclusivo; as plantas da tradição ocorrem na região. Foram feitas turnês guiadas com sete especialistas. Documentaram-se as espécies em herbário, os nomes em guarani e português, usos e partes utilizadas. Compilaram-se as espécies medicinais da literatura e dos bancos de dados dos herbários. O Fator de Consenso dos Informantes (FIC) dos usos foi comparado com artigos. As 90 espécies registradas compõem a maior lista entre artigos com indígenas do MS. Compilou-se 659 espécies medicinais usadas no MS. Das espécies compiladas, 61,1% são citadas exclusivamente no Taquara. O FIC mostrou baixa similaridade. Os Kaiowá usam misturas de plantas na medicina, o que é raramente citado. Os indígenas citam espécies que não mais ocorrem na área. Os resultados apoiam as hipóteses indicando a especificidade do conhecimento dos Kaiowá do Taquara e é um sustentáculo do reconhecimento da área ancestral.

Resumo O presente estudo apresenta a flora de Ruppiaceae no estado do Rio de Janeiro. O trabalho tem por base a análise morfológica de materiais depositados em herbários e coletas de campo, além de dados da literatura. Foi registrada uma espécie: Ruppia maritima. Descrições, ilustrações, dados sobre habitat e distribuição geográfica são apresentados.

Resumo A cultura de tecidos vegetais é imprescindível à propagação e multiplicação uniforme de plantas, à conservação de germoplasma, a programas de melhoramento e à transformação genética. Essa técnica tem exigido, cada vez mais, estudos que colaborem com o entendimento dos mecanismos envolvidos no crescimento dos microrganismos nos meios de cultivo, bem como as relações que eles estabelecem com a planta hospedeira. Dessa maneira, a presente revisão pretende esclarecer esses questionamentos e promover a distinção entre contaminação e manifestação endofítica que ocorrem no cultivo in vitro por diferentes causas. Tal distinção permite diminuir o pânico que se instala quando do seu aparecimento, além de auxiliar na adoção de medidas de prevenção e/ou controle desses eventos sem que haja descartes desnecessários de material de alto valor comercial e genético.

Resumo Apresenta-se o Horto do Colégio dos Jesuítas, na encosta do Morro do Castelo, como um local de experimentação de culturas de plantas com potencial retorno econômico, em contexto que antecede a criação do Jardim Botânico do Rio de Janeiro (1808).

Resumo Estudos sobre a família Orchidaceae no Parque Nacional da Chapada dos Veadeiros, revelou uma nova ocorrência para a flora do estado de Goiás. Bulbophyllum adiamantinum até então considerada endêmica do estado de Minas Gerais, é aqui descrita, ilustrada, comentada quanto a sua distribuição, relações morfológicas e épocas de floração e frutificação. Adicionalmente, são fornecidos um mapa com a distribuição da espécie, seu status de conservação, além de imagens e notas sobre a anatomia de seu sistema vegetativo e de B. insectiferum, com a qual pode ser confundida. Anatomicamente, ambas as espécies se diferenciam principalmente pela presença ou ausência de corpos silicosos no sistema vegetativo, de idioblastos traqueoidais no mesofilo, pelo arranjo das fibras e aspecto do lume nas células da exoderme, endoderme, periciclo e medula nas raízes. Os caracteres que mostraram-se variáveis foram o número de feixes vasculares, o formato das células da epiderme e a espessura da cutícula das espécies estudadas.

Abstract The chemical profile of Dalbergia ecastaphyllum has been indicated as the botanical origin of Brazilian red propolis, an apicultural product with proven therapeutic properties. However, few studies have investigated this plant species. This study evaluated and compared microbiological quality, chemical composition, and antioxidant activity of stem and leaf samples of D. ecastaphyllum. The samples were collected in February 2015, in the southern region of the state of Bahia, Brazil. We performed the microbiological analyses, determined the contents of fatty acid, total phenol and flavonoid, and identified the chemical profile and antioxidant activit. Escherichia coli, Salmonella spp. and sulfite reducing clostridial spores were not detected in the samples. Acids of the family ω3 were recorded in the stems and ω6 in the leaves. The leaves presented better nutritional quality of the fraction, better antioxidant capacity in the tests by the DPPH method and β-carotene bleaching. There were 49 chemical compounds, of which 38 belonged to the class of flavonoids. The results indicate that stems and leaves of D. ecastaphyllum have biological properties. Leaves particularly are better for functional food formulation and as natural antioxidant.

Abstract Cissus erosa (Vitaceae), popularly known in Brazil as Cipó-fogo, is a medicinal plant used in the treatment of warts and external ulcers. The present study aimed to evaluate the activity of stems and leaves ethanol extracts of C. erosa against the Dengue and Zika virus by the MTT colorimetric method and to carry on the phytochemical characterization of active extracts by high performance liquid chromatography coupled to mass spectrometry (UPLC-MS). Only the leaves ethanol extract showed anti-Dengue virus activity EC50 18.2 µg/ml (SI > 27.5) and low cytotoxicity for LLCMK2 cells (CC50 > 500 mg/ml). Both extracts (stems and leaves) showed anti-Zika virus activity with EC50 of 45.8 mg/ml and 82.8 mg/ml, respectively. These extracts presented CC50 of 309.2 µg/ml (leaves) and 387.6 µg/ml (stems) to Vero cells. Analysis by CCD and HPLC-DAD detected the presence of triterpenes, steroids, flavonoids and tannins. UPLC-MS analyses of these extracts, allowed the identification of the majority of flavonoids present known as vitexin, isovitexin, orientin, isoorientin and two flavones derivatives, methoxyluteolin-6(8)-C-hexosyl and luteolin-7,4’-di-O-glycosylflavone. The results of the phytochemical studies here described suggest that flavonoids and terpenoids are the substances that contribute to the antiviral activity of the ethanol extracts within this species.

Abstract Myrcia splendens is popularly known as “guamirim-de-folha-miúda”, and its occurrence ranges from Mexico to southern Brazil. The aim of this work was to identify and quantify phenolic compounds in the crude hydroalcoholic (EBH), ethyl acetate (EBAE) and dichloromethane (EBDM) extracts using the HPLC-ESI- MS/MS. In total, 15 compounds, including protocatecuic acid, syringic acid, p-coumaric acid, salicylic acid, isoquercetin, ellagic acid, ferulic acid, umbelliferone, coniferaldehyde, sinapaldehyde, carnosol, gallic acid, syringaldehyde, umbelliferone, coniferaldehyde, myricetin and kaempferol were identified. Ellagic acid was the major compound in all extracts.

Abstract Due to increased UV radiation on the Earth’s surface, caused by depletion of the stratospheric ozone, people have become more susceptible to different types of skin damage, such as erythema, sunburns, and cancer; this is especially of concern in tropical countries. Thus, efforts to improve awareness as well as the use of sunscreen are increasing worldwide. However, synthetic UV filters have been associated with deleterious effects such as photosensitization. Natural products have been used by ancient cultures for several purposes, including protecting the skin from the sun. However, there is still doubt today whether photoprotection is a real phenomenom or whether it is simply tanning of the skin. Plants have self-protective mechanisms and produce secondary metabolites that can protect themselves from UV radiation. Yet, can phytochemical compounds protect human skin? This review discusses the paradoxical effect of chemical UV filters and the influence of phytochemicals in in vitro and in vivo tests of photoprotection.

Abstract Crude non-volatile oleoresin from Burseraceae species (breu), a non-timber forest product of the Amazon, still lacks methods for its quality control even though its pharmacological activities have been associated to the major triterpenes α-amyrin (αAA), β-amyrin, α-amyrenone, β-amyrenone and lupeol (LU). Its chemical characterization has usually been determined by gas chromatography (GC) using columns with phases of low polarity, which are ineffective in the separation of αAA and LU. The present study aimed to develop a GC method to characterize and quantify triterpenes in commercial breu with good selectivity and resolution. Columns with phases of different polarity (DB-35, DB-1701, DB-17HT and DB-Innowax) were tested, and DB-17HT separated αAA and LU without signal suppression or high retention of other constituents. This column allowed the quantification of breu triterpenes using flame ionization detection and cholesterol as a reference standard. Good similarity between the calibration curves of cholesterol and αAA was verified. Internal and external standardizations led to different (p < 0.05) quantifications, but the latter showed less deviation (RSD ≤ 3%). This work provides a reproducible GC method for the quality control of commercial breu, clearly evidencing the presence of lupeol and allowing the accurate quantification of major triterpenes.

Abstract In popular medicine Silybum marianum is used as a hepatoprotective agent. Silymarin is the major constituent. The present work deals with the formulation and evaluation of S. marianum tablets from ethanolic extract by direct compression. The ethanolic extract was obtained from seeds by soxhlet extraction. Two pharmaceutical formulations were prepared using fluid extract as an active principle, and Aeroperl® 300 Pharma as a carrier. In order to improve flow ability and compressibility, co-processed excipients MicroceLac® 100 and FlowLac® 90 were employed. Pre-compression and post-compression parameters were evaluated according to USP 34-NF 29. Besides, silymarin was determined by NMR spectral data. Both formulations showed excellent rheological properties and the best biopharmaceutical parameters were observed in F2 (S. marianum ethanolic extract, aeroperl® 300 Pharma, flowLac® 90, glycolate starch and magnesium stearate) in terms of the friability (0.82 %) and the disintegration time (8.05 min).

Abstract Thirty-six compounds were identified from aerial parts of Solidago chilensis cultivated at PAF/FIOCRUZ campus in Rio de Janeiro city (RJ) using solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) analysis. These compounds are mainly non-oxygenated mono and sesquiterpenes. Germacrene D is the major compound of two the essential oil analyzed samples (12 to 23%) and of two of the volatiles samples analyzed by SPME (central stems and dry inflorescences samples). Limonene is the major compound in the fresh inflorescences sample (about 21%). The bornil acetate is present in both volatile fractions (16%) and essential oils (7-8%). All the essential oils samples evaluated shown a great antinociceptive activity, considering the used dose of the samples (1 mg/kg) and the standard substance (50 mg/kg). Solidago chilensis is one of Brazil arnicas and it is a medicinal plant widely used by the brazilian population. Other plants of the genus Solidago are also used in traditional medicine in North America and Europe. This is the first report of SPME analysis from Solidago genus and of the antinociceptive effect from Solidago chilensis essential oil.

Abstract This paper aims to investigate, for the first time, in vitro antitubercular, antileishmanial and antiproliferative activities of essential oils (EOs) from S. odoratissima leaves and flowers - grown in midwestern Brazil - against Mycobacterium tuberculosis, promastigote forms of Leishmania amazonensis and human tumor cell lines. Antimycobacterial activity of EOs was evaluated in terms of the minimal inhibitory concentration (MIC). EOs from leaves and flowers showed to be active antimicrobials against M. tuberculosis, since MIC values were 150 µg/mL and 162.5 µg/mL, respectively. Both EOs exhibited significant activity against promastigote forms of L. amazonensis; IC50 values (50% growth inhibition) were 14.36 ± 2.02 (EOs from leaves) and 19.89 ± 2.66 µg/mL (EOs from flowers). Antiproliferative activity in normal (GM07492A, lung fibroblasts) and tumor (MCF-7, HeLa and M059J) cell lines was performed by the XTT assay; results were expressed as IC50 (50% cell growth inhibition) and the selective index was calculated. IC50 values of EOs from leaves and flowers obtained in normal cell lines for were 502.97 ± 40.33 µg/mL and 370.60 ± 2.01 µg/mL, respectively. Antiproliferative activity was observed against human tumor cell lines, whose IC50 values were significantly lower than those obtained in normal cell lines of MCF-7 cells (367.57 ± 4.46 µg/mL-EOs from leaves and 357.70 ± 1.85 µg/mL-EOs from flowers) and M059J cells (492.53 ± 56.67 µg/mL-EOs from leaves and 324.90 ± 6.72 µg/mL-EOs from flowers), thus, indicating selectivity. These in vitro results showed that EOs from S. odoratissima may be an antimycobacterial, antiparasitic and antitumor agent.

Abstract Senna rugosa (Fabaceae) is a common specie of the Brazilian territory, especially in the Cerrado biome. It is widely used in the popular medicine although not yet adequately investigated as to its phytoconstituents and pharmacological activities. In this study, the preliminary phytochemical analysis of ethanolic leaf extract of S. rugosa was performed and its cytotoxicity, antitumoral and antimicrobial activities (antibacterial, antifungal, anti-Trypanosoma and anti-Leishmania) were evaluated. The crude extract was analyzed in HPLC-DAD and fractions were also characterized using GC-MS and ESI-MS techniques. Results indicate phenolic compounds majority presence, including flavonol, anthraquinone and anthrones derivatives. Relative antimicrobial activities were detected against Staphylococcus aureus, Pseudomonas aeruginosa, Candida famata, Candida krusei and Candida tropicalis. As well as, a weak anti-trypanosomatids activity against M2269 strain and BH46 strain of Leishmania sp. A relative antitumoral activity was also identified but in counterpoint were also observed a toxicity in fibroblast cells (L929). Phytochemically, results provide evidence that phenolic compounds in S. rugosa leaves might be the responsible for its antimicrobial activity and cell toxicity inferred in our research.

Abstract Lafoensia pacari has been used in traditional folk medicine in Brazilian Cerrado to treat wounds. It is important to develop studies that can clarify how the biological activity of L. pacari occurs. The aim of this study was to evaluate the healing activity of L. pacari leaves extract in an animal model. The extract was prepared from plants cultivated in Goiania-GO, Brazil. The healing activity was assayed using cutaneous wound model in rats, and macroscopic, morphometric and histological analysis of wounds were also conducted. The presence of hydrolysable tannins was detected in thin layer chromatography and in high performance liquid chromatography analysis, may be suggested the presence of ellagitannins. In the evaluation of cutaneous wounds in rats it was possible to observe that the treatment with 10% (w/v) L. pacari extract provided a reduction in the time of cutaneous wound healing, with a significant increase in variables involved in healing, such as the number of blood vessels and collagen production. Therefore, this study shows that L. pacari wound healing potential may be related to the presence of ellagitannins and corroborates to ethnopharmacological reports regarding this plant.

Abstract Rhabdomyosarcoma (RMS) is the most common soft-tissue tumour in children and adolescents. It originates in normal skeletal muscle from myogenic cells that have failed to fully differentiate, and it usually has a poor prognosis. Current RMS therapy has many adverse effects. Hence, new treatments are needed. Various pharmacological properties, such as analgesic, antineoplastic, antimicrobial, and antiparasitic properties, have been demonstrated in species of the Solanaceae family. We performed ethanolic extraction from leaves of Nicotiana glauca (Solanaceae), and the extract was successively partitioned with n-hexane, chloroform, and ethyl acetate. We evaluated the effects of extracts on RMS cells, and we found that the extracts trigger apoptosis. By bio-guided fractionation assays, we identified the apoptotic agents. Morphological assessment after apoptotic cell induction of cultured cells, mitochondrial and nuclear morphology by Mitotracker, and 4,6-diamidino-2-phenylindole (DAPI) staining, respectively, were analysed in fluorescent microscopy. The capacity of the cells to migrate or proliferate was analysed by the Petit assay, followed by methylene blue staining. NMR and GC-MS spectrometry were used to identify palmitic acid and scopoletin as the phytochemicals responsible for the observed effects. These results indicate that these compounds are apoptotic inducers and they could be useful as chemotherapeutic agents against muscle tumours.

Abstract The potential of a plant with medicinal and nutritional properties, Anredera cordifolia, is reviewed. Its common names include “Bertalha” and “folha gorda” and it is popularly used for wound healing and against fungal infections and other types of infection. Its pharmacological properties have been widely investigated and acknowledged, especially with regard to its antibacterial activity, which improves the healing of wounds infected by Staphylococcus aureus, and to its antifungal activity against Candida albicans. It is an unconventional food plant, with leaves and aerial tubers used as food prepared in varied ways. It is also considered an invasive plant in several countries and thus classified as a weed. Its characteristics of a Brazilian native plant, with proven medicinal properties and unconventional use as food, underlie our study on its ecology and botanical classification, as well as the pharmacological assays and screening of chemical constituents.

Abstract Species in the Solidago genus are used worldwide due to the therapeutic activities of their flavonoids and terpenoids. Its anti-inflammatory, antirheumatic, analgesic, diuretic, aquaretic, wound-healing properties as well as its ability to relieve burn and insect bites allow use in a variety of situations. This study was carried out to determine the metabolic profile of Solidago canadensis inflorescences (SCAI) to identify the species commercialized in Rio de Janeiro, RJ, Brazil, and evaluate the medicinal potential compared to S. chilensis and SCAI, which is used in North America and Europe. The UFLC-PDA-ESI-TOF revealed the metabolic profile of SCAI, finding the pseudo-molecular ions and characteristic fragments, mainly flavonols (10) such as quercetin, kaempferol, isorhamnetin, and its glycosides, as well as chlorogenic acid (CA). Eleven substances were identified, confirming the compatibility of the metabolic profile presented in varieties canadensis and scabra of european S. canadensis. The both contain the same flavonoid aglycones, and their glycosides are important chemical and biological markers for extracts and products based on species of the genus Solidago. UFLC-PDA-ESI-TOF characterized the extracts, which can help with quality control of raw plant materials and commercialized phytotherapeutics as well as for development of new products and phytomedicines.