RCAAP Repository
Educação, docência e memórias da professora Maria Bronzeado Machado (1940-1986)
To recognize the importante of the woman on the development of society, perhaps it is the idea that better expresses the need to introduce and to include as a subject to be studied, while an agent in history. Under this perspective, the current study longs to identify Teacher and Poet Maria Bronzeado Machado, concept of education and teaching, between 1940 and 1986. As a methodological contribution, we used the biographical method, having as sources texts found on “ A União” newspaper, on the book "Flôres do Caminho", of her own authorship, as well as accounts from former students found on social networks such as Orkut and Facebook. This study is based on contributions from Nova História Cultural which allowed an extension of approaches and methods used for research involving women. Within the perspective of representation, Roger Chartier develops useful notions and concepts to guide this investigation. Referring to the history of women, the discussion about methodologicaltheoretical references is anchored on authors such as Perrot (2008), Del Priore (2004), Louro (2012), Almeida (1997), Lima (2007), Pinsk (2014) and Rago (1991). Still authors who express ideas referred to the categories that orient the work: Biography and memory. The sources are divided into two supports: printed, which encompass news related to the teacher and poet, discourse and poems, both published by "A União" newspaper, the book Flôres do Caminho, from which, allusive texts to education, family, formation and management were analyzed, and documents related to IPEP, researched at the archives of the Paraiba State Technical Teaching Office (Extinct School Department - ESD); digital sources from where were selected texts produced by her former students, accounts about the Teacher and Poet Maria Bronzeado Machado and various practices developed at school that comprise: Curriculum, gender, religion, teaching practices, teachers, sociability, etc. The sum of these sources were necessary to understand the trajectory and performance of the teacher used as reference in the educational environment for the studied period. Her writings reveal her passion for the magistrate, her nationalist, maternal and religious educational concept. It's a belief that to analyze the memories of Teacher and Poet Maria Bronzeado Machado converge for the comprehension not only in the sense of her educational concepts and practices but also to contribute and reveal historical aspects of the education and the woman from Paraiba.
2019
Santos, Adriana Vilar dos
Representações sociais dos professores das creches das Universidades Federais paraibanas sobre arte na educação infantil
This thesis was carried out at the Pediatric Education at the Children Education Unit (UEI) of the Federal University of Campina Grande (UFCG) and School of Basic Education (EEbas) of the Federal University of Paraíba (UFPB), and had as target to know the social representations of teachers about the Art in Education for Children, identify practices and artistic skills developed in educational institutions investigated, and reflect on the art contributions to the integral development of the child. We defend the thesis in which the concept the teacher of early childhood education has of art and its implications for the overall development process of the child, are reflected in their pedagogical ways with the artistic languages. The theoretical foundations of this methodological investigative study were qualitative research which uses the theoretical and methodological framework of Social Representation Theory in the Moscovician perspective, which allowed us to approach the subjectivity of the participants and thus access to their representations. The survey data was constructed from the results collected from interviews with 11 teachers of schools / kindergartens. We chose to do a data merge with quantitative and qualitative resources used for triangulation that allowed a broader view of the phenomenon studied. Data was analyzed qualitatively, with the Bardin content analysis technique and showed that teachers shared the same representations of art, which can be explained by educational experiences and common experiences with art in action education with young children. We highlight some representations found: Art is an area composed of various knowledge specific languages; the art is human communication and expression; or that art is everything that is created by man; teaching art is an educational activity, mediation, facilitating tool in the teachers repertoire of pedagogical practice; Art interferes with the development process and formation of children through the development of creativity, autonomy, as well as its self value; freedom of expression, broadens their world view, among others. The present work does not exhaust the possibility of future studies on the subject. However, we hope that it will contribute to the discussions on working with art in children's education and their contributions to the integral formation of the human being.
2019
Lima, Norma Maria de
Crianças, infâncias e culturas infantis : epistemologias e subjetividades em narrativas fotoetnográficas
This research promotes epistemological and empirical interlocutions on the conceptions of children, childhood and children's cultures through photoetnographic narratives. It holds discussions within the classical approaches of Sociology, Visual Anthropology and Photoetnography in dialogue with the current epistemological perspectives of the Sociology of Childhood that breaks a new social paradigm in modern Science for the studies of the child. Methodologically it is characterized as a transdisciplinary research of interpretative approach and photoetnographic method, that combined different strategies of generation, treatment and data analysis. In view of this, the memento analysis and interpretation movement was initially proposed, which was confronted and discussed in the light of the interpretive, transdisciplinary and critical-cultural rigor of Visual Culture, having its implications discussed in three complementary analytical fields: the first one comprises the image, photography and Photoetnography; the second relates to the Social History of Childhood, the Sociology of Childhood and Visual Culture; and the third presents Photoetnography in an autobiographical perspective as a research strategy with children. Each analytical field required specific methodological strategies, ranging from documentary research, photonnographic research and autobiographical photoetnographic research, the latter of which had as subject one 6-year-old children living in João Pessoa. Thus, the production of photoetnographic narratives in each field of analysis allowed the knowledge, deepening and interpretive disclosure of the reality of childhood reflected in the image of the child thus legitimizing itself as a potential methodological strategy to stimulate the interpretation and understanding of identities, subjectivities and alterities of the child produced in the inter-places and between-cultures of childhood. In view of this, it was found that the photoetnographic narratives of children, in each historical time and cultural context reveal and legitimize a creative and subversive protagonism in the relationships of otherness constituted during childhood, thus, children have developed forms of participation and coping with cultural and social circumstances in which he participates, which is the defense of a new sociological image - that of the child protagonist, a child who subverts the limits and impositions of visual cultures and creatively reinvents ways of being and living in the contemporary world.
2019
Coelho, Fernanda Mendes Cabral Albuquerque
Gestão do apoio acadêmico estudantil à luz de Alice: “Somos todos loucos aqui”
This work aimed to study the management of student academic support at the Federal University of Paraíba, with the general objective of understanding how this management has been developed in the Coordination of the Pedagogy course at the Education Centre of this University. In order to carry out this research, the adventures of Alice, drawn from the world literature classics written by Lewis Carroll and published in 1865 and 1871, were used as background. The fall in the rabbit's hole and other situations lived by Alice in the Wonderland and Through the Looking Glass were initially the scenario for the description of the paths taken to define the research theme. These attempts are related to the management of student academic support because they are located in the practical field of the planned activities (and some already developed) in the position of Technical in Educational Affairs in UFPB. Then, from one of Alice's best-known dialogues with the Cheshire Cat, the maxim "we're all crazy here" was used to construct the theoretical referential. Based on the studies of CUNHA (1999), CHAUÍ (2001), SANTOS (2004, 2005), COULON (2008, 2017) and CHARLOT (2005), it was built the framework for understanding the contradictions and complexities involved in the Higher Education Management and for the description of the world of instabilities, transformations and novelties to be experienced by students upon entering a university. Regarding the research focus, the methodological principles of qualitative research were used through the case study. The research was carried out through the study and analysis of the documents related to the management of academic support in the UFPB and in the Coordination of the Course of Pedagogy at the Education Center. All the documentary analysis was permeated by reports of cases and practices already experienced by the Coordination of the Course of Pedagogy and, in this sense, were also presented and discussed some possibilities already offered by the existing support instruments for academic student management: the Management System of Academic Activities (SIGAA) and Heritage and Administration of Contracts (SIPAC). The results indicate that there are many difficulties related to the interpretation and experience of the rules defined by the documents and instruments related to the management of student academic support in the UFPB (the confusion of "game with living things", as the Queen's Croquet Ground). It is concluded that from the understanding of the complexity of the management of higher education, one can - as Alice learns from her experiences - find the most appropriate sizes to support the student who needs to cross an environment of perplexities that shows at all times. In the paths of academic management it is necessary to change and build perspectives, to learn and to unlearn continuously. From this conception, some proposals for possible improvements have been made, while still believing in those that seem impossible.
2019
Leite, Gabriela de Carvalho
Gasto público e execução orçamentária: uma análise da aplicação de recursos na área de extensão no âmbito da Reitoria do Instituto Federal da Paraíba – IFPB, no período de 2015 a 2017
This research is a study on Public Expenditure and Budget Execution: a analysis of the application of resources in the area of extension within the Rectory of the Institute Federal University of Paraíba - IFPB in the period from 2015 to 2017. The present study objective of analyzing the budget execution of expenditures directed to the area of in the scope of the Rectory of the Federal Institute of Paraíba (IFPB), using as a temporal cutoff the period from 2015 to 2017 and is composed of the following objectives specific: map documents and legislations pertaining to extension in the IFPB; to examine execution of the budget credit for the extension by nature of expenditure in the within the Rectorate of the IFPB; to relate the available budget credit to the real implementation of the budget for expenditures for the extension in the scope of the Rectory of the IFPB; and verify the implementation of IFPB extension projects from the perception of its coordinators and of employees involved in the management budgeting. This study is characterized by the qualitative approach, being descriptive, bibliographical and documentary research, with the objective of subsidize the theoretical information, as well as data obtained through reports extracted from the Management Treasury. The questionnaire was used as a collection tool along with extension project coordinators and servers involved in the management both financial ties to the Rectory of the IFPB. Finally, the present The study presented the execution of the budget credits for the activities of within the scope of the Rector's Office of the IFPB, verifying that the available executed in its entirety, even considering a significant budget cut as a result of economic policy measures. Concerning execution of extension projects based on the perception of their coordinators and servers involved in budgetaryfinancial management, it is pointed out as relevant aspects the enhancement of monitoring mechanism, such as the Treasury Management System, ensuring the efficiency and effectiveness of budget execution, as well as a effort for the extension project announcements to be prepared in accordance with the budgetary and financial programming of the institution, avoiding its discontinuity. On the other hand, the analysis of the data revealed some challenges: the credit destined extension projects are insufficient to meet the demands; late payment scholarships, impacting negatively on the development of activities and the extension be left in the third plane to the detriment of teaching and research, affecting the principle of inseparability.
2019
Netto, João Correia Lira
Aspectos clínico-epidemiológicos, diagnóstico e controle da infecção por Trypanosoma vivax em rebanho bovino no estado do Maranhão
In the state of Maranhão, breeding cattle is important in the region's economy and is growing, but in properties in the municipality of Timon there are reports that some animals present clinical signs suggestive of trypanosomiasis. The objective of this study was to investigate the occurrence of trypanosomiasis in a dairy farm in this municipality Of Timon, state of Maranhão. The owner reported abortus, births of weak calves, and mortality of adult animals with progressive weight loss. Visits to the property were carried out to obtain the history, realize animal examination and blood collection for the Woo test, hemograms, serological tests for trypanosomiasis, leptospirosis, and neosporosis and PCR for molecular diagnosis of T. vivax. The identification of animals with low values in the hematocrit was the main hematological alteration identified in the herd. Two animals were positive in the Woo test, and trypanosomes were visualized in blood smears, confirmed by molecular diagnosis as T. vivax. It was identified that 95.23% (40/42) of the animals with low hematocrit were serologically positive for T. vivax. The conditions identified in the property as an environment propitious to mechanical vectors, the presence of wild animals and the introduction of animals from states where trypanosomiasis outbreaks had already been reported were probably associated with the introduction and dissemination of the agent in the herd. The occurrence of abortions without the manifestation of other clinical signs of trypanosomiasis suggests that these could also be associated with other diseases, such as leptospirosis, since high titers were identified in the animals tested. The high number of serologically positive animals for trypanosomiasis 82.51% (151/183) shows that almost all the herd had contact with the agent. The good nutritional status of the animals and the adoption in the herd of adequate sanitary measures probably ensured an efficient immune response at the time of initial contact of the animals with the agent, avoiding high rates of morbidity and mortality. The rapid establishment of control measures, including the use of trypanocidal drugs, contributed to the control of the outbreak. No more deaths and significant improvement was observed in the animals with symptoms of the disease. Despite the difficulty in accounting for the damages resulting from the diseases, it was observed that these were very expressive. The study allowed verifying the occurrence of the first outbreak of Trypanosoma vivax in the state of Maranhão, Brazil. The environmental condition identified in the property, associated with the intensification of animal husbandry practices, favored the occurrence of the trypanosomiasis and caused serious productive losses. The clinical diagnosis of the disease was difficult by the similarity of the clinical signs of trypanosomiasis with other diseases and the possibility of association of two or more diseases in the same patient, which emphasizes the importance of establishing adequate diagnostic measures as a way to avoid the dissemination of the disease and to minimize the economic losses of the producers.
2019
Pereira, Hélio Domingos
Neosporose em bovinos de corte criados extensivamente na zona rural da mata paraibana
Studies on identification and diagnosis of neosporosis in cattle has been increasing worldwide, being characterized as an important differential diagnosis for reproductive problems, resulting in considerable economic losses. This paper reports a case of congenital and poliradiculoneurite encephalomyelitis Neospora caninum–associated in a 6-months-old male calf, which diagnosis was confirmed by PCR after euthanasia. This is the longest survival time reported of a congenitally infected calf by Neospora caninum and highlight the need of begin Paraiba´s herds screening as soon as possible to identify their sanity status. This way, the second study aimed to determine the presence of anti-N. caninum antibodies in a beef cattle herd in Paraiba “Zona da Mata” mesorregion. Were tested 68 samples for presence of anti-N. caninum antibodies by Indirect Immunofluorescence Reaction in Infectious Diseases Laboratory at the Federal Rural University of Pernambuco. A total of 66.2% (45/68) were negative and 33.8% (23/68) were positive. Antibody titers ranged from 200 up to 3,200. The high presence of anti-N. caninum antibodies associated to history of reproductive problems, show agent's presence in the herd and highlight the urgency for more research in Paraíba state, including other species involved in the epidemiology of infection for better control and prevent to reduce losses.
2019
Marques, Michele Flávia Sousa
Osteodistrofia fibrosa nutricional em jacarés de cativeiro no Estado da Paraíba
Crocodilians are subdivided in three families: Alligatoridae, Crocodylidae and Gavialidae in a total of 23 species. When young they feed small fish and crustaceans, in adulthood consume vertebrates including fish, turtles, birds and mammals. Metabolic bone disease involves a group of pathological conditions developed as result of prolonged deficiencies in calcium, phosphorus and vitamin D. Fibrous osteodystrophy is a metabolic bone disease most commonly found in captive reptiles. The pathogenesis occurs by stimulation of the parathyroid glands due to decrease in plasma concentrations of calcium, stimulating the secretion of parathyroid hormone as a compensatory mechanism. Reports of problems on nutritional management are constant, however few information is available for these species. In this study, the diagnosis of fibrous osteodystrophy in 17 caimans from three different species was based on epidemiological aspects, clinical history, radiographic alterations, and mainly macroscopic and histopathological findings. Radiographs revealed osteopenia, bone thinning, tortuosity of the vertebrae and fractures areas. In the necropsy were observed variables degrees of bone fragility, spinal deformities, bilateral symmetrical enlargement of the long bones and parathyroid markedly increased. The histopathological findings consisted of marked rarefaction of bone matrix with severe thinning from maxillary and frontal bone forming numerous cystic areas with islands of cartilage calcified and intense replacement by fibrous tissue. The absence of injury at the renal parenchyma and parathyroid tumors in all animals confirm the diagnosis of fibrous osteodystrophy by nutritional secondary hyperparathyroidism. After feeding correction with 0.1 ml of calcium gluconate for each animal orally, no additional cases of disease was observed.
2019
Nascimento, Harlan Hallamys de Lima
Mastite estafilocócica por MRSA em pequenos ruminantes no Estado de OHIO - EUA
Mastitis has a large impact on the economy as well as on animal welfare in sheep and goat production. While several microorganisms can cause mastitis in small ruminants, Staphylococcus aureus is one of the most important pathogen. An increasing percentage strains showing resistance against methicillin (Methicillin Resistent Staphylococcus aureus - MRSA) have been recovered from different sources and can potentially cause severe illness in humans and animals. Livestock associated MRSA (LA-MRSA) are strains often isolated from animals, veterinarians and people who are in contact to food animals and the sequence type 398 is the frequent LA-MRSA strain associated to human infections. This paper discusses mastitis cases caused by MRSA strains in goats and sheep herds in Ohio - USA. One sheep showing fever, reactive mammary lymphnodes, thinness, muscle weakness and mastitis in both mammary glands was examined. One teat had a fistula draining pus. A sample of purulent discharge was collected and sent for analisys. Phenotypical and genotypical tests were performed. Sample was nuc and mecA positive confirming genetically the Methicillin resistant S. aureus. MLST revealed that the tested MRSA isolate belonged to the clonal lineage ST398. This is the first MRSA report causing mastitis in sheep in USA. Mostly important, the identification of ST398 warrants further epidemiological investigations about epidemiology of this pathogen in animal production systems. The second study aimed to investigate occurrence of Methicillin Resistant S. aureus (MRSA) associated with intramammary infections in goat herds in Ohio State, USA. A total of 120 milk samples and 120 teat-swab samples were collected from five farms. After conventional isolating and phenotypic characterization of colonies, molecular characterization was also performed through PCR for nuc and mecA genes for MRSA confirmation and clonal complexes determined by MLST. Fifteen (6.2%) positive S. aureus samples were found in this study; nine from milk and five from teat skin samples. Four (2%) MRSA isolates were detected and belonged to clonal complexes ST133 and ST5. Three (1.25%) coagulase-negative isolates were shown to harbor the mecA gene. This study indicates 17 that MRSA belonging to clonal complex 133 can be associated with intrammamary infections in goat herds in US and the identification of the MRSA ST5 is of special concern, since it’s an important pathogen to humans.
2019
Moura, Guilherme Santana de
Modulação da expressão gênica e proteica caruncular pelo embrião bovino durante a placentação
Despite the existence of several studies related to reproduction, some events about embryo loss in early gestation are not understand due the variables involved. This study aimed to identify differentially expressed genes between uterine caruncles of the gravid horns (G) and non-gravid horns (NG) horns during the bovine placentation and investigate the role of embryo produced by in vitro fertilization (IVF) in this gene expression. The finding that the embryo also influences the caruncular gene and protein expression could clarify which embryo signals that regulate uterine development and that the findings may be related to high failure rates of pregnancy in the pregnancies of manipulated embryos. In the present study caruncles from the G and NG were collected from pregnant primiparous cows (Bos indicus) undergoing artificial insemination (AI) or transfer of embryos produced by in vitro fertilization (IVF) using Bos indicus male sexed sperm. Animals were slaughter at 30 (n = 3), 35 (n = 8) or 40 (n = 3) days of gestation and tissues were collected following separation of the weakly associated cotyledons. Tissues were either frozen in liquid nitrogen or stored at -80°C freezer until RNA or protein extraction or fixed in 4% buffered formaldehyde for immunohistochemical analysis. The transcriptome of samples of 35 days (n = 7) were evaluated by microarray using an Affymetrix microarray platform. Analysis showed that 23.000 genes, 149 were differentially expressed in cattle caruncles from the gravid horn (≥ 1.5 fold, p<0.05). Nine genes potentially involved in cell differentiation were used to validate the results of real time PCR: seven upregulated genes (ACP5,DPP4, GJB6, IGFBP3, INHBA, STC1,THBS2) and two downregulated genes (CXCR4 e PTGS2). Quantitative PCR demonstrated that expression ratios of selected genes were consistent with the microarray results (p<0,05). Western blot analysis was performed to investigate PTGS2, THSB2 e IGFPB3. Densitometric evaluation indicated an increase in protein content of PTGS2 at 40 days and IGFPB3 and THSB2 at 35 ABSTRACT 4 and 40 days of IA both gestations. In FIV gestations, PTGS2 protein abundance was significantly higher at 35 days in caruncles from the gravid horn. On the other hand, the pattern of IGFPB3 e THSB2 protein expression in FIV gestations was similar to that observed in IA gestations. Protein immunostaining was observed in the cytoplasm of epithelial and stromal uterine cells, glandular uterine cells and endothelial uterine cells. We observed that staining intensity was associated to western blot data, with cells from cotyledon-associated caruncle showing more intensity of staining at 35 and 40 days in both AI and FIV gestations. We conclude that the expression of placentation involved genes differentially expressed in caruncles of pregnant and non-pregnant horns suffer influence of embryo and procedures such as in vitro embryo production and cell culture can influence the regulation of these molecules, since the greatest expression of most genes in IVF pregnancies occurred later after 40 days.
2019
Jesus, Carla Fabiana Gomes de
Avaliação dos parâmetros hematológico e bioquímicos de codornas japonesas (Coturnix coturnix japonica) em diferentes faixas etáriass
The Japanese quail industry is increasing in expansion in Brazil and has become a very important activity in the Brazilian economic scenario, so it has been generated jobs and income for the population. However, it notes a lack of knowledge about blood and biochemical constituents of the specie. Thus, the study aimed to evaluate the influence of age on hematological and biochemical parameters of Japanese quail (Coturnix coturnix japonica). Seven samples were collected with quail at 7, 14, 21, 28, 35, 42 and 80 days old. In total, 224 quail were evaluated wherever 32 quail per collection. Each bird was individually weighed. The blood sample was collected by cardiac puncture, collecting about 3 mL blood. The complete blood count was performed with 1 mL blood, using 20 μL of EDTA 5% for each mL of blood. The remaining volume was placed in a tube without anticoagulant to obtain serum after centrifugation. Then the birds were euthanized. The bursa of Fabricius, thymus, spleen and liver were weighed on a precision scale. It was calculated normal ratio of heterophil:lymphocyte (H/L) and it was measured the total area of 290 erythrocytes per age group. The images of the erythrocytes were obtained and analyzed by digital camera (Moticam 2500) coupled to the optical Zeiss microscope equipped with an image analysis system (Motic Images Plus Version 2.0). The serum was measured for concentrations of total protein, Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), Gamma glutamyl transferase (GGT), Alkaline Phosphatase (ALP), triglycerides, total cholesterol, urea, uric acid and calcium by the automatic biochemical analyzer Mindray (BS-120) using commercial reagent kits (Labtest Diagnóstica S.A.®). First, it was applied to the groups the Shapiro-Wilk normality test through the statistical program BioEstat 5.0. Then, the comparison between two groups was made by t-test or the Mann-Whitney U. Finally, the comparison of more than two groups was taken by Tukey test or viii Kruskal-Wallis test at 5% significance level. Birds with 80 days of age showed an involution of the spleen, thymus and bursa of Fabricius. Young birds showed lower average values of packed cell volume, red blood cells and hemoglobin concentration. Furthermore, they showed red blood cells in larger size and concomitantly higher average values of mean corpuscular volume. Also, showed heterophils as the predominant leukocyte and consequently higher H/L. It is noteworthy that there appears to be a correlation in the number of circulating lymphocytes and weight of the lymphoid organs. Quails with 80 days of age showed higher average values of total serum protein, calcium and lipids. It was found a linear decrease of average values of ALP activity in quails between 7 and 80 days of age. We conclude that there are variations in hematological and biochemical parameters of Japanese quails in accordance with age. Therefore, the results of this study serve as a warning for the evaluation of hematological parameters of the specie, as these variations are physiological and should not be associated with diseases.
2019
Bernardino, Maria das Graças da Silva
Soroepidemiologia de neospora caninum em caprinos (Capra hircus) do Estado da Paraíba
The neosporosis is recognized as a protozoan infection caused by Neospora caninum. It is primarily associated with cases of abortion and neonatal mortality in cattle. In Brazil, several studies focusing on this agent have been developed, however in the Northeast there are few studies about this disease and its impact on goat herds. The aim of this study was to investigate the prevalence of anti N. caninum antibodies in goats from Paraíba state. It was collected 406 goat blood samples (35 males and 371 females) from 8 different farms belonging to rural areas from Paraíba state. A total of 106/406 (26.11%; 95% CI 21.96 to 30.72%) were seropositive for indirect immunofluorescence antibody assay (IFA). It was considered positive Titers≥ 1:50. There was no significant association between location, age, gender, presence of dogs on the property, occurrence of abortion and seropositivity for N. caninum (p> 0.05). Thus, due to this high prevalence, it is suggested that this infection is common in goats in the State of Paraíba. Therefore studies about this protozoan are extremely important, seeking effective control strategies based on the assessment of risk factors.
2019
Braz., Beatriz Maria de Almeida
Modulação da expressão gênica caruncular pelo embrião bovino durante a placentação: Influência sobre fatores angiogênicos e vasoativos
In cattle, reproductive loss occurs mainly embryonic period (0-42 days), and one of the major cause of this issue are failures in synchronization of maternal and embryonic communication. The conceptus (embryo and extraembryonic membranes) plays a key role in controlling intrauterine environment, leading to the release of paracrine factors for gestational signaling and modulating the expression of genes in maternal endometrium, leading up to implantation and subsequent early placental development. Despite of in vitro production of embryos proves to be a useful tool in increasing productivity of cattle, this method still has gaps in successful of reproductive efficiency. This study aimed to analyze the expression of genes involved with placental vascular function in gravid and non-gravid uterine horns of primiparous cows submitted to artificial insemination (AI) and in vitro fertilization (IVF), in order to identify the regulatory role of embryo on endometrial gene expression and the influence of in vitro embryonic manipulation on this mechanism. In this study, caruncular samples of the gravid horn were collected after the separation of fetal cotyledons, occurring on days 30 (n = 3), 35 (n = 8) and 40 (n = 3) AI and 35 (n = 3) and 40 (n = 3) days IVF. Caruncles of contralateral uterine horns were collected concomitantly. All tissues were frozen in liquid nitrogen and stored at - 80°C until RNA extraction. Four genes (eNOS, GUCY1B3, EDNRB, ANGPT2) involved with placental vascular function were chosen for this study. All samples were processed by Quantitative Real Time PCR (qPCR), at where the analysis of results showed a significant increase (p<0.05) in AI gravid horns between 30 to 35 days for both EDNRB and ANGPT2 genes, while GUCY1B3 was upregulated on gravid horns only at 30 days AI. Expression levels of eNOS did not changed in AI uterine tissues. Late transcription response occurred at 40 days IVF on gravid horns for eNOS, EDNRB and ANGPT2, and no significant alterations (p <0.05) was observed for GUCY1B3. Considering the observed aspects, we conclude that the differential expression of genes on gravid horns has a direct relation with the presence of embryo, being these genes themselves liable to modifications in transcriptional pattern due to adversities imposed by manipulation and culture of in vitro embryos.
2019
Cordeiro, Laysa Lindaura Lau Rocha
Informatio complex: a complexidade da informação ambiental e a promoção do desenvolvimento humano
Tout d’abod on cherche la nature et le rôle de l’information ambientale en tant que véhicule de promotion du développement humain dans la société contemporaine. Il s’agit d’une étude théorique et descriptive concernant le domaine de la Science de l’Information (SI) lequel adopte la pensée complexe comme schèma théorique et perspective épistemologique et aussi la transdisciplinarité en tant qu’orientation méthodologique. D’emblée on essaie de caracteriser l’environnement et la société contemporaine comme étant, respectivement, moyen informationnel et société d’information, afin de souligner le privilège de la visée téchniciste et la limitation de l’exercice interdisciplinaire de la SI, lorsqu’elle se rapporte à l’information ambientale et leurs implications sur le developpement. Toutefois, on cherche une alternative viable par le biais de la visée humaniste présente dans la pensée complexe, tout en réalisant une revision de la théorie mathématicienne de la communication, de la cibérnetique, de la théorie genérale des systèmes et de la théorie de l’automate auto-organisé, qui sont, désormais, utilisées dans la constitution téchiniciste de la Science de l´Information. Avec cela, on caractérise le concept d’information ambientale selon Morin et, en même temps, on définit l’information ambientale comme une construction anthropobiopsicossocionoológique et physique, afin de décrire les rapports entre l’information ambientale et la promotion du developpement humain. Ainsi, on essaie de mettre en évidence les questions locales et planetaires de l’information ambientale dans la promotion du développement humain et, finalement, de présenter une proposition destinée à penser l’object de la SI à partir de sa propre complexité.
2019
Silva, Alan Curcino Pedreira da
Padrão hematológico, hormonal e morfologia godanal de gavião-carijó (Rupornis magnirostris)
Efforts on raptors conservation have been increased in last three decades, among them, we can point out breeding programs in captivity and zoos, scientific research, environmental education, rehabilitation and reintroduction. As result, more raptors have been met by veterinarians, requiring theoretical basis to perform an appropriate care, however, there is little literature available regarding Brazilian raptors reference values, both in captivity and free life. To generate such theoretical basis and minimize this gap in knowledge, blood and gonads samples of 12 Roadside hawk (Rupornis magnirostris) from Arruda Câmara Park – João Pessoa – PB, were collected in order to determine hematological parameters, hormonal values and gonadal morphology. Routine techniques were used for hematological analysis, histological and chemiluminescence for hematological determination, hormone levels and gonodal morphology. Hematological parameters found were Globular Volume: 0.36±0.04 L L-1, Red cells: 1.79±0.26 x1012 L-1, Hemoglobin: 199.55±13.01 g L-1, VGM: 199.55±13.01 fL, CHGM: 22.87 ± 1.96%, PPT: 5.15±1.19 g L-1, fibrinogen: 0.12±0.11 g L-1, leukocyte count: 13.28±5.45 x109 L-1 monocytes: 1.21±1.05 x109 L-1, Heterophile: 7.52±5.22 x109 L-1 lymphocytes: 0.73±0.64 x109 L-1 Eosinophils: : 1.43±1.38 x109 L-1 Basophils: 0.15±0.15 x109 L-1 , hormone TSH levels were 0.28±0.02 uUI mL-1, T3: 178.42±129.37 ng dL-1, T4: 1.46±1.04 mcg dL-1 FSH: 0.33±0.36 mUI mL-1, LH: 0.29±0.57 mUI mL-1, E2: 37.333±21.49 pg mL-1, Progesterone: 1.48±0.92 ng mL-1 and testosterone: 10.35±1.21 ng dL-1. Hematologic values of GV, erythrocytes and lymphocytes differ between naïve captivity birds and birds with at least one year in captivity, that difference can be associated to stress since naïve birds face a huge lifestyle change in captivity. T3 showed a significant difference between males and females and T3 and T4 were higher in moulting animals. Gonadal morphology were similar to other birds, however, was observed right ovary persistence in 66% of cases. Gonads were collected between June and July and demonstrated histological features of reproductive rest phase or beginning of proliferative period, showing same reproductive seasonality in Brazil’s northeastern, where seasons are not well defined. Results generate a Roadside Hawk hematological profile on two different occasions, when arriving in captivity and 13 after at least one year, and gonads hormonal and morphological data. Collected data plays a role as useful knowledge for clinical treatments on these birds, providing a better understanding of raptors reproduction, assisting species preservation.
2019
Dias, Glenison Ferreira
Detecção sorológica e molecular Theileria equi em equinos do Estado da Paraíba
Equine piroplasmosis is a tick borne infectious disease, whose etiologic agent Theileria equi is an obligatory erythrocyte parasite. Thus, the aim of this study was to evaluate the prevalence and molecular data of T. equi in horses from urban and rural areas in six cities of Paraiba state, in northeastern Brazil. Blood samples from 119 animals were collected in order to evaluate total protein values, globular volume and also performing indirect immunofluorescence assay (IFA) and polymerase chain reaction (PCR). Of all the animals studied, 61, 6% (85/119) were seropositive at IFAT and 49, 6% (59/119) were positive in PCR. In the evaluation of epidemiological surveys for analysis of risk factors, there was no significant association between the presence of ticks, age or sex with seropositivity or molecular detection of T. equi (p> 0.05). The prevalence of the infection in serological and molecular analysis when compared with hematocrit values less than 32% was 68.9% and 64.4%, respectively. The horses that had increased total plasma protein (> 7.9 g / dL) was 66, 38% in serology and 52,33% molecular analysis. Thus, it is need to control ticks, monitoring the health status of animals and preparation of active surveillance programs to prevent the spread of this parasite to susceptible animals.
2019
Ribeiro, Luana Paula da Silva
As novas dinâmicas socioespaciais no espaço urbano da cidade de Cajazeiras-PB, como resultantes da presença do Ensino Superior
This research sought to address higher education government of Fernando Henrique Cardoso – FHC (1995-2002), and Luiz Inácio Lula da Silva (2003-2010), with regard to the process of expansion, extension and internalization of higher education (HELs), Brazilian public and private, as well as the courses from public policies implemented by these managements for those managements for the promotion and development of this subsector. That said, the general objective of this study it’s to analyze the dynamics socioespaciais in the city of Brazil, as resulting from the presence of the public and private higher education. Higher education in cajazeirense territory in the early 1970, however, just came to develop and consolidate from the decade of 1980 and the first decade of the years 2000, respectively, depending on the implementation of the teacher training Center of the Federal University of Paraíba (CFP/UFPB), in 1980, and with the expansion and extension of IES and subsequent courses to the year 2006. The methodology of approach and procedures assumed continuous and contradictory changes revealed in the form and content of the phenomena deployed in the city of Brazil, as a reflection of the higher education subsector. Thus, dialectical and historical materialism led our theoretical and methodological reflection to realize our goals, primary and secondary. We gave importance to the qualitative analysis, defining the bibliographical research, documentation, field, data and information collection, as being the main procedures of that research. The findings from this study allow us to affirm that the presence of higher education triggered changes in morphology and in the contents of the intraurbano space of Cajazeiras.
2019
Costa Filho, Joaquim Alves da
Avaliação espaço-temporal do processo de desertificação em sub-bacias hidrográficas do Rio Paraíba no Semiárido do Brasil
Remote Sensing is an essential tool for the detection,monitoring and evaluation of desertification. In this context, this study analyzed the relationship between SAVI and precipitation to detect changes in vegetation cover caused by the desertification process in the period 1986-2017 in the sub-basins of the Paraíba River, Northeast Brazil. For this, a descriptive statistical analysis, Pearson correlation and an adaptation of the RESTREND method using SAVI (Landsat 30 m) andspatiallyexplicitraindata(CHIRPS)wereperformed. Theresultsshowthatduringthestudy period, the precipitation distribution varied between 350 and 400 mm, obtaining coefficients of variationgreaterthan30%. Theprecipitationbehaviorindicated5yearsofdryseason(DS),6dry years (D), 10 neutral years (N), 6 rainy years (R) and 5 rainier years (RA). The spatial variation of the mean SAVI was higher (> 0.7) mainly in the western portion and in some spots located in more pronounced topographies of the study area. In contrast, SAVI pixels <0.3 followed the major rivers in the study area. The RA years (SAVI = 0.85±0.14,min = 0.71,max = 0.87) and R (SAVI = 0.80±0.15,min = 0.64,max = 0.96) present the highest values of SAVI. It is observed that in D years (SAVI = 0.62±0.14,min = 0.53,max = 0.69) and DS (SAVI = 0.50±0.15,min = 0.37,max = 0.67) have the lowest values. SAVI and precipitation were significantly correlated (p≤0.05) to 83.17% of the pixels and mean correlation coefficient was 0.53. The SAVI trend indicated that 58.57% of the pixels presented significant increasing trends (p≤0.05)ofthepixelvaluesand34.04%ofthepixelsobtainedsignificantdecreasingtendencies (p≤0.05). Residual SAVI (adjusted by precipitation) had a negative residual tendency observed in 31.41% of the pixels and 26.75% of the pixels showed a positive residual trend. During 19862001, a negative residual trend was observed in 50.34% of the pixels and 38.48% of the pixels showed a positive residual trend. In the period 2002-2017, a negative trend was observed in 34% of the pixels and 16% of the pixels showed a positive trend. The SAVI intercept precipitation generally remained above zero, where in the western portion obtained values above 0.5 per pixel. In sum, precipitation impacts and human activities on vegetation dynamics varied in the studied area and specific local measures of environ mental protection and managements hould be adopted.
Qualitative properties of positive singular solutions to nonlinear elliptic systems with critical exponent
In this work we study the asymptotic behavior to positive solutions of the following coupled elliptic system of nonlinear Schrödinger equations ∆gui − 2 X j=1 Aij(x)uj + n(n−2) 4 |U| 4 n−2ui = 0 which are defined in the punctured unit ball B1(0)\{0} for n ≥ 3. Here g is a Riemannian metric on the unit ball and the potential A is assumed a C1 map such that Aij(x) is a symmetrical matrix for each x in B1(0). From the viewpoint of conformal geometry, this systems are pure extensions of Yamabe-type equations. We will approach the problem assuming first that g is the euclidian metric and the potential A vanishes. In this case we are able to prove that the solutions of our problem are asymptotics to what we call Fowler-type solutions. In the general case we will prove the same result by putting some restrictions on the potential and assuming that the dimension is less or equal to five.
2019
Caju, Rayssa Helena Aires de Lima
Conhecimento botânico local de plantas medicinais em uma comunidade rural no Agreste da Paraíba (Nordeste do Brasil)
The present study registered the use, knowledge and importance of the medicinal plants used by the residents of the rural community of Palmeira, in the Municipality of Cuitegi-PB, Northeastern Brazil. Semi-structured interviews were conducted with 74 informants (heads of families), aged between 23 and 98 years, 24 men and 50 women. The interviewees signed the free and informed consent form (Resolution 196/96). The IR, VUgeneral, VUcorrent and VUpotential, was applied. The botanical material collected in the field was identified, processed and incorporated in the Herbarium Jaime Coelho de Morais (CCA-UFPB-Campus II). It was used to compare VUgeneral, VUcorrent, VUpotential the Wilcoxon test. To evaluate the relationship and similarities between the VUgeneral, VUcorrent, VUpotencial and IR, the Spearman correlation test was performed. A total of 1482 citations and 142 species (72 exotics and 70 native) were found, similar values were found, belonging to 64 botanical families. The most cited species are Lippia alba (Mill.) N.E.Br. (Lemon balm) n = 65 (4.39%). Cymbopogon citratus (DC.) Stapf (Capim santo) n = 63 (4.25%). The most prominent botanical families were Fabaceae n = 15 (10.56%) and Lamiaceae n = 12 (8.45%). The leaves were the most used part of the plant n = 738 (49.80%). The Decoct obtained the largest number of citations n = 423 (28.54%). The therapeutic indications reported by the informants obtained 98 different citations of use. Among the conditions mentioned, those presenting the highest numbers were: cough n = 273 (18.42%), belly pain n = 184 (12.42%) and disorders of the digestive system n = 315 (25.78%). It was identified that the backyards is an environment in which the families cultivate plants n = 815 (54.99%). Lippia alba (Mill.) N.E.Br. (Lavender) VUgeneral (0,88). Current value for money Lippia alba (Mill.) N.E.Br. (VUcorrent = 0.73) and the potential value of Anacardium occidentale L. (Caju-roxo) (VUpotential = 0.55). The most versatile plant was the Mentha x piperita L (Mineral leafminer) (IR = 2) showing the highest number of therapeutic indication citations. Significant differences were found between VUgeneral and VUcorrent and, between VUcorrent and VUpotencial. No significant difference was observed between VUgeneral and VUpotencial. However, there was a significant positive relationship between all indexes tested in the research. There are no significant statistical differences regarding the age group of the women and the species cited and origin. However, there are significant differences between the group of the women and the therapeutic indications. Through the analyzes carried out, it is understood that the medicinal plants are impotant for the residents of the community of Palmeira, being used for various therapeutic purposes mainly for the treatment of affections that reach the respiratory and respiratory systems.